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Annexins A2 and A5 are potential early biomarkers of hepatocarcinogenesis

Hepatocellular carcinoma (HCC) is a highly lethal liver cancer with late diagnosis; therefore, the identification of new early biomarkers could help reduce mortality. We determine the tissue and plasma status of five annexins during hepatocarcinogenesis by diethylnitrosamine-induced cirrhosis-HCC. W...

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Autores principales: Herrera-López, Ema Elvira, Guerrero-Escalera, Dafne, Aguirre-Maldonado, Isaac, López-Hernández, Arely, Montero, Hilda, Gutiérrez‐Nava, María Angélica, del Pozo-Yauner, Luis, Arellanes-Robledo, Jaime, Camacho, Javier, Pérez-Carreón, Julio Isael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10147597/
https://www.ncbi.nlm.nih.gov/pubmed/37117324
http://dx.doi.org/10.1038/s41598-023-34117-8
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author Herrera-López, Ema Elvira
Guerrero-Escalera, Dafne
Aguirre-Maldonado, Isaac
López-Hernández, Arely
Montero, Hilda
Gutiérrez‐Nava, María Angélica
del Pozo-Yauner, Luis
Arellanes-Robledo, Jaime
Camacho, Javier
Pérez-Carreón, Julio Isael
author_facet Herrera-López, Ema Elvira
Guerrero-Escalera, Dafne
Aguirre-Maldonado, Isaac
López-Hernández, Arely
Montero, Hilda
Gutiérrez‐Nava, María Angélica
del Pozo-Yauner, Luis
Arellanes-Robledo, Jaime
Camacho, Javier
Pérez-Carreón, Julio Isael
author_sort Herrera-López, Ema Elvira
collection PubMed
description Hepatocellular carcinoma (HCC) is a highly lethal liver cancer with late diagnosis; therefore, the identification of new early biomarkers could help reduce mortality. We determine the tissue and plasma status of five annexins during hepatocarcinogenesis by diethylnitrosamine-induced cirrhosis-HCC. We found that Anxa5 was the earliest upregulated gene at week 12 after HCC initiation, while Anxa1 and Anxa2 were upregulated in advanced HCC stages (weeks 18 and 22). Furthermore, the protein level of Annexin A1, A2, A5 and A10 was increased from the early stages. Immunofluorescence and subcellular fractionation revealed Annexin A1, A2, and A5 in the cytoplasm and nuclei of tumor cells. Notably, increased plasma levels of Annexin A5 significantly (r(2) = 0.8203) correlated with Annexin A5 levels in liver tissue from week 12 and gradually increased until week 22. Using the TCGA database, we found that the expression of ANXA2 (HR = 1.7, p = 0.0046) and ANXA5 (HR = 1.8, p = 0.00077) was associated with poor survival in HCC patients. In conclusion, we have identified Annexin A1 and A5 as potentially useful early biomarkers for poor prognosis in HCC patients.
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spelling pubmed-101475972023-04-30 Annexins A2 and A5 are potential early biomarkers of hepatocarcinogenesis Herrera-López, Ema Elvira Guerrero-Escalera, Dafne Aguirre-Maldonado, Isaac López-Hernández, Arely Montero, Hilda Gutiérrez‐Nava, María Angélica del Pozo-Yauner, Luis Arellanes-Robledo, Jaime Camacho, Javier Pérez-Carreón, Julio Isael Sci Rep Article Hepatocellular carcinoma (HCC) is a highly lethal liver cancer with late diagnosis; therefore, the identification of new early biomarkers could help reduce mortality. We determine the tissue and plasma status of five annexins during hepatocarcinogenesis by diethylnitrosamine-induced cirrhosis-HCC. We found that Anxa5 was the earliest upregulated gene at week 12 after HCC initiation, while Anxa1 and Anxa2 were upregulated in advanced HCC stages (weeks 18 and 22). Furthermore, the protein level of Annexin A1, A2, A5 and A10 was increased from the early stages. Immunofluorescence and subcellular fractionation revealed Annexin A1, A2, and A5 in the cytoplasm and nuclei of tumor cells. Notably, increased plasma levels of Annexin A5 significantly (r(2) = 0.8203) correlated with Annexin A5 levels in liver tissue from week 12 and gradually increased until week 22. Using the TCGA database, we found that the expression of ANXA2 (HR = 1.7, p = 0.0046) and ANXA5 (HR = 1.8, p = 0.00077) was associated with poor survival in HCC patients. In conclusion, we have identified Annexin A1 and A5 as potentially useful early biomarkers for poor prognosis in HCC patients. Nature Publishing Group UK 2023-04-28 /pmc/articles/PMC10147597/ /pubmed/37117324 http://dx.doi.org/10.1038/s41598-023-34117-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Herrera-López, Ema Elvira
Guerrero-Escalera, Dafne
Aguirre-Maldonado, Isaac
López-Hernández, Arely
Montero, Hilda
Gutiérrez‐Nava, María Angélica
del Pozo-Yauner, Luis
Arellanes-Robledo, Jaime
Camacho, Javier
Pérez-Carreón, Julio Isael
Annexins A2 and A5 are potential early biomarkers of hepatocarcinogenesis
title Annexins A2 and A5 are potential early biomarkers of hepatocarcinogenesis
title_full Annexins A2 and A5 are potential early biomarkers of hepatocarcinogenesis
title_fullStr Annexins A2 and A5 are potential early biomarkers of hepatocarcinogenesis
title_full_unstemmed Annexins A2 and A5 are potential early biomarkers of hepatocarcinogenesis
title_short Annexins A2 and A5 are potential early biomarkers of hepatocarcinogenesis
title_sort annexins a2 and a5 are potential early biomarkers of hepatocarcinogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10147597/
https://www.ncbi.nlm.nih.gov/pubmed/37117324
http://dx.doi.org/10.1038/s41598-023-34117-8
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