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Air pollution nanoparticle and alpha-synuclein fibrils synergistically decrease glutamate receptor A1, depending upon nPM batch activity

BACKGROUND: Epidemiological studies have variably linked air pollution to increased risk of Parkinson’s disease (PD). However, there is little experimental evidence for this association. Alpha-synuclein (α-syn) propagation plays central roles in PD and glutamate receptor A1 (GluA1) is involved in me...

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Autores principales: Zhang, Hongqiao, D'Agostino, Carla, Tulisiak, Christopher, Thorwald, Max A., Bergkvist, Liza, Lindquist, Allison, Meyerdirk, Lindsay, Schulz, Emily, Becker, Katelyn, Steiner, Jennifer A., Cacciottolo, Mafalda, Kwatra, Mohit, Rey, Nolwen L., Escobar Galvis, Martha L., Ma, Jiyan, Sioutas, Constantinos, Morgan, Todd E., Finch, Caleb E., Brundin, Patrik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10148131/
https://www.ncbi.nlm.nih.gov/pubmed/37128335
http://dx.doi.org/10.1016/j.heliyon.2023.e15622
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author Zhang, Hongqiao
D'Agostino, Carla
Tulisiak, Christopher
Thorwald, Max A.
Bergkvist, Liza
Lindquist, Allison
Meyerdirk, Lindsay
Schulz, Emily
Becker, Katelyn
Steiner, Jennifer A.
Cacciottolo, Mafalda
Kwatra, Mohit
Rey, Nolwen L.
Escobar Galvis, Martha L.
Ma, Jiyan
Sioutas, Constantinos
Morgan, Todd E.
Finch, Caleb E.
Brundin, Patrik
author_facet Zhang, Hongqiao
D'Agostino, Carla
Tulisiak, Christopher
Thorwald, Max A.
Bergkvist, Liza
Lindquist, Allison
Meyerdirk, Lindsay
Schulz, Emily
Becker, Katelyn
Steiner, Jennifer A.
Cacciottolo, Mafalda
Kwatra, Mohit
Rey, Nolwen L.
Escobar Galvis, Martha L.
Ma, Jiyan
Sioutas, Constantinos
Morgan, Todd E.
Finch, Caleb E.
Brundin, Patrik
author_sort Zhang, Hongqiao
collection PubMed
description BACKGROUND: Epidemiological studies have variably linked air pollution to increased risk of Parkinson’s disease (PD). However, there is little experimental evidence for this association. Alpha-synuclein (α-syn) propagation plays central roles in PD and glutamate receptor A1 (GluA1) is involved in memory and olfaction function. METHODS: Each mouse was exposed to one of three different batches of nano-particulate matter (nPM) (300 μg/m(3), 5 h/d, 3 d/week), collected at different dates, 2017–2019, in the same urban site. After these experiments, these nPM batches were found to vary in activity. C57BL/6 female mice (3 mo) were injected with pre-formed murine α-synuclein fibrils (PFFs) (0.4 μg), which act as seeds for α-syn aggregation. Two exposure paradigms were used: in Paradigm 1, PFFs were injected into olfactory bulb (OB) prior to 4-week nPM (Batch 5b) exposure and in Paradigm 2, PFFs were injected at 4th week during 10-week nPM exposure (Batches 7 and 9). α-syn pSer129, microglia Iba1, inflammatory cytokines, and Gria1 expression were measured by immunohistochemistry or qPCR assays. RESULTS: As expected, α-syn pSer129 was detected in ipsilateral OB, anterior olfactory nucleus, amygdala and piriform cortex. One of the three batches of nPM caused a trend for elevated α-syn pSer129 in Paradigm 1, but two other batches showed no effect in Paradigm 2. However, the combination of nPM and PFF significantly decreased Gria1 mRNA in both the ipsi- and contra-lateral OB and frontal cortex for the most active two nPM batches. Neither nPM nor PFFs alone induced responses of microglia Iba1 and expression of Gria1 in the OB and cortex. CONCLUSION: Exposures to ambient nPM had weak effect on α-syn propagation in the brain in current experimental paradigms; however, nPM and α-syn synergistically downregulated the expression of Gria1 in both OB and cortex.
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spelling pubmed-101481312023-04-30 Air pollution nanoparticle and alpha-synuclein fibrils synergistically decrease glutamate receptor A1, depending upon nPM batch activity Zhang, Hongqiao D'Agostino, Carla Tulisiak, Christopher Thorwald, Max A. Bergkvist, Liza Lindquist, Allison Meyerdirk, Lindsay Schulz, Emily Becker, Katelyn Steiner, Jennifer A. Cacciottolo, Mafalda Kwatra, Mohit Rey, Nolwen L. Escobar Galvis, Martha L. Ma, Jiyan Sioutas, Constantinos Morgan, Todd E. Finch, Caleb E. Brundin, Patrik Heliyon Research Article BACKGROUND: Epidemiological studies have variably linked air pollution to increased risk of Parkinson’s disease (PD). However, there is little experimental evidence for this association. Alpha-synuclein (α-syn) propagation plays central roles in PD and glutamate receptor A1 (GluA1) is involved in memory and olfaction function. METHODS: Each mouse was exposed to one of three different batches of nano-particulate matter (nPM) (300 μg/m(3), 5 h/d, 3 d/week), collected at different dates, 2017–2019, in the same urban site. After these experiments, these nPM batches were found to vary in activity. C57BL/6 female mice (3 mo) were injected with pre-formed murine α-synuclein fibrils (PFFs) (0.4 μg), which act as seeds for α-syn aggregation. Two exposure paradigms were used: in Paradigm 1, PFFs were injected into olfactory bulb (OB) prior to 4-week nPM (Batch 5b) exposure and in Paradigm 2, PFFs were injected at 4th week during 10-week nPM exposure (Batches 7 and 9). α-syn pSer129, microglia Iba1, inflammatory cytokines, and Gria1 expression were measured by immunohistochemistry or qPCR assays. RESULTS: As expected, α-syn pSer129 was detected in ipsilateral OB, anterior olfactory nucleus, amygdala and piriform cortex. One of the three batches of nPM caused a trend for elevated α-syn pSer129 in Paradigm 1, but two other batches showed no effect in Paradigm 2. However, the combination of nPM and PFF significantly decreased Gria1 mRNA in both the ipsi- and contra-lateral OB and frontal cortex for the most active two nPM batches. Neither nPM nor PFFs alone induced responses of microglia Iba1 and expression of Gria1 in the OB and cortex. CONCLUSION: Exposures to ambient nPM had weak effect on α-syn propagation in the brain in current experimental paradigms; however, nPM and α-syn synergistically downregulated the expression of Gria1 in both OB and cortex. Elsevier 2023-04-19 /pmc/articles/PMC10148131/ /pubmed/37128335 http://dx.doi.org/10.1016/j.heliyon.2023.e15622 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Zhang, Hongqiao
D'Agostino, Carla
Tulisiak, Christopher
Thorwald, Max A.
Bergkvist, Liza
Lindquist, Allison
Meyerdirk, Lindsay
Schulz, Emily
Becker, Katelyn
Steiner, Jennifer A.
Cacciottolo, Mafalda
Kwatra, Mohit
Rey, Nolwen L.
Escobar Galvis, Martha L.
Ma, Jiyan
Sioutas, Constantinos
Morgan, Todd E.
Finch, Caleb E.
Brundin, Patrik
Air pollution nanoparticle and alpha-synuclein fibrils synergistically decrease glutamate receptor A1, depending upon nPM batch activity
title Air pollution nanoparticle and alpha-synuclein fibrils synergistically decrease glutamate receptor A1, depending upon nPM batch activity
title_full Air pollution nanoparticle and alpha-synuclein fibrils synergistically decrease glutamate receptor A1, depending upon nPM batch activity
title_fullStr Air pollution nanoparticle and alpha-synuclein fibrils synergistically decrease glutamate receptor A1, depending upon nPM batch activity
title_full_unstemmed Air pollution nanoparticle and alpha-synuclein fibrils synergistically decrease glutamate receptor A1, depending upon nPM batch activity
title_short Air pollution nanoparticle and alpha-synuclein fibrils synergistically decrease glutamate receptor A1, depending upon nPM batch activity
title_sort air pollution nanoparticle and alpha-synuclein fibrils synergistically decrease glutamate receptor a1, depending upon npm batch activity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10148131/
https://www.ncbi.nlm.nih.gov/pubmed/37128335
http://dx.doi.org/10.1016/j.heliyon.2023.e15622
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