Cargando…

Injectable hypoxia-preconditioned cartilage progenitor cells-laden GelMA microspheres system for enhanced osteoarthritis treatment

Osteoarthritis (OA) is the most common age-related degenerative joint disease mainly characterized by the destruction of articular cartilage. Owing to its native avascular property, intrinsic repair of articular cartilage is very limited. Thus, a chondrogenic microenvironment in the joint is essenti...

Descripción completa

Detalles Bibliográficos
Autores principales: Feng, Kai, Yu, Yifan, Chen, Zhengsheng, Wang, Feng, Zhang, Kunqi, Chen, Hongfang, Xu, Jia, Kang, Qinglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10148185/
https://www.ncbi.nlm.nih.gov/pubmed/37128287
http://dx.doi.org/10.1016/j.mtbio.2023.100637
_version_ 1785034944923303936
author Feng, Kai
Yu, Yifan
Chen, Zhengsheng
Wang, Feng
Zhang, Kunqi
Chen, Hongfang
Xu, Jia
Kang, Qinglin
author_facet Feng, Kai
Yu, Yifan
Chen, Zhengsheng
Wang, Feng
Zhang, Kunqi
Chen, Hongfang
Xu, Jia
Kang, Qinglin
author_sort Feng, Kai
collection PubMed
description Osteoarthritis (OA) is the most common age-related degenerative joint disease mainly characterized by the destruction of articular cartilage. Owing to its native avascular property, intrinsic repair of articular cartilage is very limited. Thus, a chondrogenic microenvironment in the joint is essential to the preservation of healthy chondrocytes and OA treatment. Recently, cartilage progenitor cells (CPCs)-based therapy is emerging as a promising strategy to repair degenerated and damaged articular cartilage. In this study, injectable hypoxia-preconditioned three-dimensional (3D) cultured CPCs-laden gelatin methacryloyl (GelMA) microspheres (CGMs) were constructed and characterized. Compared to normoxia-pretreated 3D CPCs and two-dimensional (2D) cultured CPCs, hypoxia-preconditioned 3D cultured CPCs exhibited enhanced cartilage extracellular matrix (ECM) secretion and greater chondrogenic ability. In addition, hypoxia-preconditioned 3D cultured CPCs more effectively maintained cartilage matrix metabolism balance and attenuated articular cartilage degeneration in subacute and chronic rat OA models. Mechanistically, our results demonstrated hypoxia-preconditioned 3D cultured CPCs exerted chondro-protective effects by inhibiting inflammation and oxidative stress via NRF2/HO-1 pathway in vitro and in vivo. Together, through the 3D culture of CPCs using GelMA microspheres (GMs) under hypoxia environment, our results proposed an efficient articular cartilage regeneration strategy for OA treatment and could provide inspiration for other stem cells-based therapies.
format Online
Article
Text
id pubmed-10148185
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-101481852023-04-30 Injectable hypoxia-preconditioned cartilage progenitor cells-laden GelMA microspheres system for enhanced osteoarthritis treatment Feng, Kai Yu, Yifan Chen, Zhengsheng Wang, Feng Zhang, Kunqi Chen, Hongfang Xu, Jia Kang, Qinglin Mater Today Bio Full Length Article Osteoarthritis (OA) is the most common age-related degenerative joint disease mainly characterized by the destruction of articular cartilage. Owing to its native avascular property, intrinsic repair of articular cartilage is very limited. Thus, a chondrogenic microenvironment in the joint is essential to the preservation of healthy chondrocytes and OA treatment. Recently, cartilage progenitor cells (CPCs)-based therapy is emerging as a promising strategy to repair degenerated and damaged articular cartilage. In this study, injectable hypoxia-preconditioned three-dimensional (3D) cultured CPCs-laden gelatin methacryloyl (GelMA) microspheres (CGMs) were constructed and characterized. Compared to normoxia-pretreated 3D CPCs and two-dimensional (2D) cultured CPCs, hypoxia-preconditioned 3D cultured CPCs exhibited enhanced cartilage extracellular matrix (ECM) secretion and greater chondrogenic ability. In addition, hypoxia-preconditioned 3D cultured CPCs more effectively maintained cartilage matrix metabolism balance and attenuated articular cartilage degeneration in subacute and chronic rat OA models. Mechanistically, our results demonstrated hypoxia-preconditioned 3D cultured CPCs exerted chondro-protective effects by inhibiting inflammation and oxidative stress via NRF2/HO-1 pathway in vitro and in vivo. Together, through the 3D culture of CPCs using GelMA microspheres (GMs) under hypoxia environment, our results proposed an efficient articular cartilage regeneration strategy for OA treatment and could provide inspiration for other stem cells-based therapies. Elsevier 2023-04-17 /pmc/articles/PMC10148185/ /pubmed/37128287 http://dx.doi.org/10.1016/j.mtbio.2023.100637 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Full Length Article
Feng, Kai
Yu, Yifan
Chen, Zhengsheng
Wang, Feng
Zhang, Kunqi
Chen, Hongfang
Xu, Jia
Kang, Qinglin
Injectable hypoxia-preconditioned cartilage progenitor cells-laden GelMA microspheres system for enhanced osteoarthritis treatment
title Injectable hypoxia-preconditioned cartilage progenitor cells-laden GelMA microspheres system for enhanced osteoarthritis treatment
title_full Injectable hypoxia-preconditioned cartilage progenitor cells-laden GelMA microspheres system for enhanced osteoarthritis treatment
title_fullStr Injectable hypoxia-preconditioned cartilage progenitor cells-laden GelMA microspheres system for enhanced osteoarthritis treatment
title_full_unstemmed Injectable hypoxia-preconditioned cartilage progenitor cells-laden GelMA microspheres system for enhanced osteoarthritis treatment
title_short Injectable hypoxia-preconditioned cartilage progenitor cells-laden GelMA microspheres system for enhanced osteoarthritis treatment
title_sort injectable hypoxia-preconditioned cartilage progenitor cells-laden gelma microspheres system for enhanced osteoarthritis treatment
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10148185/
https://www.ncbi.nlm.nih.gov/pubmed/37128287
http://dx.doi.org/10.1016/j.mtbio.2023.100637
work_keys_str_mv AT fengkai injectablehypoxiapreconditionedcartilageprogenitorcellsladengelmamicrospheressystemforenhancedosteoarthritistreatment
AT yuyifan injectablehypoxiapreconditionedcartilageprogenitorcellsladengelmamicrospheressystemforenhancedosteoarthritistreatment
AT chenzhengsheng injectablehypoxiapreconditionedcartilageprogenitorcellsladengelmamicrospheressystemforenhancedosteoarthritistreatment
AT wangfeng injectablehypoxiapreconditionedcartilageprogenitorcellsladengelmamicrospheressystemforenhancedosteoarthritistreatment
AT zhangkunqi injectablehypoxiapreconditionedcartilageprogenitorcellsladengelmamicrospheressystemforenhancedosteoarthritistreatment
AT chenhongfang injectablehypoxiapreconditionedcartilageprogenitorcellsladengelmamicrospheressystemforenhancedosteoarthritistreatment
AT xujia injectablehypoxiapreconditionedcartilageprogenitorcellsladengelmamicrospheressystemforenhancedosteoarthritistreatment
AT kangqinglin injectablehypoxiapreconditionedcartilageprogenitorcellsladengelmamicrospheressystemforenhancedosteoarthritistreatment