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Protocol to prepare doubly labeled fluorescent nucleosomes for single-molecule fluorescence microscopy

Single-molecule fluorescence microscopy (SMFM) has been shown to be informative in understanding the interaction of chromatin-associated factors with nucleosomes, the basic building unit of chromatin. Here, we present a protocol for preparing doubly labeled fluorescent nucleosomes for SMFM. We descr...

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Detalles Bibliográficos
Autores principales:	Ghoneim, Mohamed, Musselman, Catherine A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10148226/ https://www.ncbi.nlm.nih.gov/pubmed/37083320 http://dx.doi.org/10.1016/j.xpro.2023.102229

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