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Pocket to concavity: a tool for the refinement of protein–ligand binding site shape from alpha spheres

SUMMARY: Understanding the binding site of the target protein is essential for rational drug design. Pocket detection software predicts the ligand binding site of the target protein; however, the predicted protein pockets are often excessively estimated in comparison with the actual volume of the bo...

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Autores principales: Kudo, Genki, Hirao, Takumi, Yoshino, Ryunosuke, Shigeta, Yasuteru, Hirokawa, Takatsugu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10148677/
https://www.ncbi.nlm.nih.gov/pubmed/37086438
http://dx.doi.org/10.1093/bioinformatics/btad212
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author Kudo, Genki
Hirao, Takumi
Yoshino, Ryunosuke
Shigeta, Yasuteru
Hirokawa, Takatsugu
author_facet Kudo, Genki
Hirao, Takumi
Yoshino, Ryunosuke
Shigeta, Yasuteru
Hirokawa, Takatsugu
author_sort Kudo, Genki
collection PubMed
description SUMMARY: Understanding the binding site of the target protein is essential for rational drug design. Pocket detection software predicts the ligand binding site of the target protein; however, the predicted protein pockets are often excessively estimated in comparison with the actual volume of the bound ligands. This study proposes a refinement tool for the pockets predicted by an alpha sphere-based approach, Pocket to Concavity (P2C). P2C is divided into two modes: Ligand-Free (LF) and Ligand-Bound (LB) modes. The LF mode provides the shape of the deep and druggable concavity where the core scaffold can bind. The LB mode searches the deep concavity around the bound ligand. Thus, P2C is useful for identifying and designing desirable compounds in Structure-Based Drug Design (SBDD). AVAILABILITY AND IMPLEMENTATION: Pocket to Concavity is freely available at https://github.com/genki-kudo/Pocket-to-Concavity. This tool is implemented in Python3 and Fpocket2.
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spelling pubmed-101486772023-04-30 Pocket to concavity: a tool for the refinement of protein–ligand binding site shape from alpha spheres Kudo, Genki Hirao, Takumi Yoshino, Ryunosuke Shigeta, Yasuteru Hirokawa, Takatsugu Bioinformatics Applications Note SUMMARY: Understanding the binding site of the target protein is essential for rational drug design. Pocket detection software predicts the ligand binding site of the target protein; however, the predicted protein pockets are often excessively estimated in comparison with the actual volume of the bound ligands. This study proposes a refinement tool for the pockets predicted by an alpha sphere-based approach, Pocket to Concavity (P2C). P2C is divided into two modes: Ligand-Free (LF) and Ligand-Bound (LB) modes. The LF mode provides the shape of the deep and druggable concavity where the core scaffold can bind. The LB mode searches the deep concavity around the bound ligand. Thus, P2C is useful for identifying and designing desirable compounds in Structure-Based Drug Design (SBDD). AVAILABILITY AND IMPLEMENTATION: Pocket to Concavity is freely available at https://github.com/genki-kudo/Pocket-to-Concavity. This tool is implemented in Python3 and Fpocket2. Oxford University Press 2023-04-22 /pmc/articles/PMC10148677/ /pubmed/37086438 http://dx.doi.org/10.1093/bioinformatics/btad212 Text en © The Author(s) 2023. Published by Oxford University Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Applications Note
Kudo, Genki
Hirao, Takumi
Yoshino, Ryunosuke
Shigeta, Yasuteru
Hirokawa, Takatsugu
Pocket to concavity: a tool for the refinement of protein–ligand binding site shape from alpha spheres
title Pocket to concavity: a tool for the refinement of protein–ligand binding site shape from alpha spheres
title_full Pocket to concavity: a tool for the refinement of protein–ligand binding site shape from alpha spheres
title_fullStr Pocket to concavity: a tool for the refinement of protein–ligand binding site shape from alpha spheres
title_full_unstemmed Pocket to concavity: a tool for the refinement of protein–ligand binding site shape from alpha spheres
title_short Pocket to concavity: a tool for the refinement of protein–ligand binding site shape from alpha spheres
title_sort pocket to concavity: a tool for the refinement of protein–ligand binding site shape from alpha spheres
topic Applications Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10148677/
https://www.ncbi.nlm.nih.gov/pubmed/37086438
http://dx.doi.org/10.1093/bioinformatics/btad212
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