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Factors involved in heterologous expression of proteins in E. coli host

The production of recombinant proteins is one of the most significant achievements of biotechnology in the last century. These proteins are produced in the eukaryotic or prokaryotic heterologous hosts. By increasing the omics data especially related to different heterologous hosts as well as the pre...

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Autores principales: Pouresmaeil, Mahin, Azizi-Dargahlou, Shahnam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10148705/
https://www.ncbi.nlm.nih.gov/pubmed/37120438
http://dx.doi.org/10.1007/s00203-023-03541-9
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author Pouresmaeil, Mahin
Azizi-Dargahlou, Shahnam
author_facet Pouresmaeil, Mahin
Azizi-Dargahlou, Shahnam
author_sort Pouresmaeil, Mahin
collection PubMed
description The production of recombinant proteins is one of the most significant achievements of biotechnology in the last century. These proteins are produced in the eukaryotic or prokaryotic heterologous hosts. By increasing the omics data especially related to different heterologous hosts as well as the presence of new amenable genetic engineering tools, we can artificially engineer heterologous hosts to produce recombinant proteins in sufficient quantities. Numerous recombinant proteins have been produced and applied in various industries, and the global recombinant proteins market size is expected to be cast to reach USD 2.4 billion by 2027. Therefore, identifying the weakness and strengths of heterologous hosts is critical to optimize the large-scale biosynthesis of recombinant proteins. E. coli is one of the popular hosts to produce recombinant proteins. Scientists reported some bottlenecks in this host, and due to the increasing demand for the production of recombinant proteins, there is an urgent need to improve this host. In this review, we first provide general information about the E. coli host and compare it with other hosts. In the next step, we describe the factors involved in the expression of the recombinant proteins in E. coli. Successful expression of recombinant proteins in E. coli requires a complete elucidation of these factors. Here, the characteristics of each factor will be fully described, and this information can help to improve the heterologous expression of recombinant proteins in E. coli.
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spelling pubmed-101487052023-05-01 Factors involved in heterologous expression of proteins in E. coli host Pouresmaeil, Mahin Azizi-Dargahlou, Shahnam Arch Microbiol Original Paper The production of recombinant proteins is one of the most significant achievements of biotechnology in the last century. These proteins are produced in the eukaryotic or prokaryotic heterologous hosts. By increasing the omics data especially related to different heterologous hosts as well as the presence of new amenable genetic engineering tools, we can artificially engineer heterologous hosts to produce recombinant proteins in sufficient quantities. Numerous recombinant proteins have been produced and applied in various industries, and the global recombinant proteins market size is expected to be cast to reach USD 2.4 billion by 2027. Therefore, identifying the weakness and strengths of heterologous hosts is critical to optimize the large-scale biosynthesis of recombinant proteins. E. coli is one of the popular hosts to produce recombinant proteins. Scientists reported some bottlenecks in this host, and due to the increasing demand for the production of recombinant proteins, there is an urgent need to improve this host. In this review, we first provide general information about the E. coli host and compare it with other hosts. In the next step, we describe the factors involved in the expression of the recombinant proteins in E. coli. Successful expression of recombinant proteins in E. coli requires a complete elucidation of these factors. Here, the characteristics of each factor will be fully described, and this information can help to improve the heterologous expression of recombinant proteins in E. coli. Springer Berlin Heidelberg 2023-04-29 2023 /pmc/articles/PMC10148705/ /pubmed/37120438 http://dx.doi.org/10.1007/s00203-023-03541-9 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Paper
Pouresmaeil, Mahin
Azizi-Dargahlou, Shahnam
Factors involved in heterologous expression of proteins in E. coli host
title Factors involved in heterologous expression of proteins in E. coli host
title_full Factors involved in heterologous expression of proteins in E. coli host
title_fullStr Factors involved in heterologous expression of proteins in E. coli host
title_full_unstemmed Factors involved in heterologous expression of proteins in E. coli host
title_short Factors involved in heterologous expression of proteins in E. coli host
title_sort factors involved in heterologous expression of proteins in e. coli host
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10148705/
https://www.ncbi.nlm.nih.gov/pubmed/37120438
http://dx.doi.org/10.1007/s00203-023-03541-9
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