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A phylogenomic and comparative genomic analysis of Commensalibacter, a versatile insect symbiont
BACKGROUND: To understand mechanisms of adaptation and plasticity of pollinators and other insects a better understanding of diversity and function of their key symbionts is required. Commensalibacter is a genus of acetic acid bacterial symbionts in the gut of honey bees and other insect species, ye...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10149009/ https://www.ncbi.nlm.nih.gov/pubmed/37120592 http://dx.doi.org/10.1186/s42523-023-00248-6 |
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author | Botero, Juliana Sombolestani, Atena Sadat Cnockaert, Margo Peeters, Charlotte Borremans, Wim De Vuyst, Luc Vereecken, Nicolas J. Michez, Denis Smagghe, Guy Bonilla-Rosso, German Engel, Philipp Vandamme, Peter |
author_facet | Botero, Juliana Sombolestani, Atena Sadat Cnockaert, Margo Peeters, Charlotte Borremans, Wim De Vuyst, Luc Vereecken, Nicolas J. Michez, Denis Smagghe, Guy Bonilla-Rosso, German Engel, Philipp Vandamme, Peter |
author_sort | Botero, Juliana |
collection | PubMed |
description | BACKGROUND: To understand mechanisms of adaptation and plasticity of pollinators and other insects a better understanding of diversity and function of their key symbionts is required. Commensalibacter is a genus of acetic acid bacterial symbionts in the gut of honey bees and other insect species, yet little information is available on the diversity and function of Commensalibacter bacteria. In the present study, whole-genome sequences of 12 Commensalibacter isolates from bumble bees, butterflies, Asian hornets and rowan berries were determined, and publicly available genome assemblies of 14 Commensalibacter strains were used in a phylogenomic and comparative genomic analysis. RESULTS: The phylogenomic analysis revealed that the 26 Commensalibacter isolates represented four species, i.e. Commensalibacter intestini and three novel species for which we propose the names Commensalibacter melissae sp. nov., Commensalibacter communis sp. nov. and Commensalibacter papalotli sp. nov. Comparative genomic analysis revealed that the four Commensalibacter species had similar genetic pathways for central metabolism characterized by a complete tricarboxylic acid cycle and pentose phosphate pathway, but their genomes differed in size, G + C content, amino acid metabolism and carbohydrate-utilizing enzymes. The reduced genome size, the large number of species-specific gene clusters, and the small number of gene clusters shared between C. melissae and other Commensalibacter species suggested a unique evolutionary process in C. melissae, the Western honey bee symbiont. CONCLUSION: The genus Commensalibacter is a widely distributed insect symbiont that consists of multiple species, each contributing in a species specific manner to the physiology of the holobiont host. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s42523-023-00248-6. |
format | Online Article Text |
id | pubmed-10149009 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-101490092023-05-01 A phylogenomic and comparative genomic analysis of Commensalibacter, a versatile insect symbiont Botero, Juliana Sombolestani, Atena Sadat Cnockaert, Margo Peeters, Charlotte Borremans, Wim De Vuyst, Luc Vereecken, Nicolas J. Michez, Denis Smagghe, Guy Bonilla-Rosso, German Engel, Philipp Vandamme, Peter Anim Microbiome Research BACKGROUND: To understand mechanisms of adaptation and plasticity of pollinators and other insects a better understanding of diversity and function of their key symbionts is required. Commensalibacter is a genus of acetic acid bacterial symbionts in the gut of honey bees and other insect species, yet little information is available on the diversity and function of Commensalibacter bacteria. In the present study, whole-genome sequences of 12 Commensalibacter isolates from bumble bees, butterflies, Asian hornets and rowan berries were determined, and publicly available genome assemblies of 14 Commensalibacter strains were used in a phylogenomic and comparative genomic analysis. RESULTS: The phylogenomic analysis revealed that the 26 Commensalibacter isolates represented four species, i.e. Commensalibacter intestini and three novel species for which we propose the names Commensalibacter melissae sp. nov., Commensalibacter communis sp. nov. and Commensalibacter papalotli sp. nov. Comparative genomic analysis revealed that the four Commensalibacter species had similar genetic pathways for central metabolism characterized by a complete tricarboxylic acid cycle and pentose phosphate pathway, but their genomes differed in size, G + C content, amino acid metabolism and carbohydrate-utilizing enzymes. The reduced genome size, the large number of species-specific gene clusters, and the small number of gene clusters shared between C. melissae and other Commensalibacter species suggested a unique evolutionary process in C. melissae, the Western honey bee symbiont. CONCLUSION: The genus Commensalibacter is a widely distributed insect symbiont that consists of multiple species, each contributing in a species specific manner to the physiology of the holobiont host. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s42523-023-00248-6. BioMed Central 2023-04-29 /pmc/articles/PMC10149009/ /pubmed/37120592 http://dx.doi.org/10.1186/s42523-023-00248-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Botero, Juliana Sombolestani, Atena Sadat Cnockaert, Margo Peeters, Charlotte Borremans, Wim De Vuyst, Luc Vereecken, Nicolas J. Michez, Denis Smagghe, Guy Bonilla-Rosso, German Engel, Philipp Vandamme, Peter A phylogenomic and comparative genomic analysis of Commensalibacter, a versatile insect symbiont |
title | A phylogenomic and comparative genomic analysis of Commensalibacter, a versatile insect symbiont |
title_full | A phylogenomic and comparative genomic analysis of Commensalibacter, a versatile insect symbiont |
title_fullStr | A phylogenomic and comparative genomic analysis of Commensalibacter, a versatile insect symbiont |
title_full_unstemmed | A phylogenomic and comparative genomic analysis of Commensalibacter, a versatile insect symbiont |
title_short | A phylogenomic and comparative genomic analysis of Commensalibacter, a versatile insect symbiont |
title_sort | phylogenomic and comparative genomic analysis of commensalibacter, a versatile insect symbiont |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10149009/ https://www.ncbi.nlm.nih.gov/pubmed/37120592 http://dx.doi.org/10.1186/s42523-023-00248-6 |
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