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Staphylococcus aureus strains exhibit heterogenous tolerance to direct cold atmospheric plasma therapy
The global clinical and socioeconomic impact of chronic wounds is substantial. The main difficulty that clinicians face during the treatment of chronic wounds is the risk of infection at the wound site. Infected wounds arise from an accumulation of microbial aggregates in the wound bed, leading to t...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10149328/ https://www.ncbi.nlm.nih.gov/pubmed/37138646 http://dx.doi.org/10.1016/j.bioflm.2023.100123 |
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author | Baz, Abdullah Bakri, Ahmed Butcher, Mark Short, Bryn Ghimire, Bhagirath Gaur, Nishtha Jenkins, Toby Short, Robert D. Riggio, Marcello Williams, Craig Ramage, Gordon Brown, Jason L. |
author_facet | Baz, Abdullah Bakri, Ahmed Butcher, Mark Short, Bryn Ghimire, Bhagirath Gaur, Nishtha Jenkins, Toby Short, Robert D. Riggio, Marcello Williams, Craig Ramage, Gordon Brown, Jason L. |
author_sort | Baz, Abdullah |
collection | PubMed |
description | The global clinical and socioeconomic impact of chronic wounds is substantial. The main difficulty that clinicians face during the treatment of chronic wounds is the risk of infection at the wound site. Infected wounds arise from an accumulation of microbial aggregates in the wound bed, leading to the formation of polymicrobial biofilms that can be largely resistant to antibiotic therapy. Therefore, it is essential for studies to identify novel therapeutics to alleviate biofilm infections. One innovative technique is the use of cold atmospheric plasma (CAP) which has been shown to possess promising antimicrobial and immunomodulatory properties. Here, different clinically relevant biofilm models will be treated with cold atmospheric plasma to assess its efficacy and killing effects. Biofilm viability was assessed using live dead qPCR, and morphological changes associated with CAP evaluated using scanning electron microscopy (SEM). Results indicated that CAP was effective against Candida albicans and Pseudomonas aeruginosa, both as mono-species biofilms and when grown in a triadic model system. CAP also significantly reduced viability in the nosocomial pathogen, Candida auris. Staphylococcus aureus Newman exhibited a level of tolerance to CAP therapy, both when grown alone or in the triadic model when grown alongside C. albicans and P. aeruginosa. However, this degree of tolerance exhibited by S. aureus was strain dependent. At a microscopic level, biofilm treatment led to subtle changes in morphology in the susceptible biofilms, with evidence of cellular deflation and shrinkage. Taken together, these results indicate a promising application of direct CAP therapy in combatting wound and skin-related biofilm infections, although biofilm composition may affect the treatment efficacy. |
format | Online Article Text |
id | pubmed-10149328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-101493282023-05-02 Staphylococcus aureus strains exhibit heterogenous tolerance to direct cold atmospheric plasma therapy Baz, Abdullah Bakri, Ahmed Butcher, Mark Short, Bryn Ghimire, Bhagirath Gaur, Nishtha Jenkins, Toby Short, Robert D. Riggio, Marcello Williams, Craig Ramage, Gordon Brown, Jason L. Biofilm Article The global clinical and socioeconomic impact of chronic wounds is substantial. The main difficulty that clinicians face during the treatment of chronic wounds is the risk of infection at the wound site. Infected wounds arise from an accumulation of microbial aggregates in the wound bed, leading to the formation of polymicrobial biofilms that can be largely resistant to antibiotic therapy. Therefore, it is essential for studies to identify novel therapeutics to alleviate biofilm infections. One innovative technique is the use of cold atmospheric plasma (CAP) which has been shown to possess promising antimicrobial and immunomodulatory properties. Here, different clinically relevant biofilm models will be treated with cold atmospheric plasma to assess its efficacy and killing effects. Biofilm viability was assessed using live dead qPCR, and morphological changes associated with CAP evaluated using scanning electron microscopy (SEM). Results indicated that CAP was effective against Candida albicans and Pseudomonas aeruginosa, both as mono-species biofilms and when grown in a triadic model system. CAP also significantly reduced viability in the nosocomial pathogen, Candida auris. Staphylococcus aureus Newman exhibited a level of tolerance to CAP therapy, both when grown alone or in the triadic model when grown alongside C. albicans and P. aeruginosa. However, this degree of tolerance exhibited by S. aureus was strain dependent. At a microscopic level, biofilm treatment led to subtle changes in morphology in the susceptible biofilms, with evidence of cellular deflation and shrinkage. Taken together, these results indicate a promising application of direct CAP therapy in combatting wound and skin-related biofilm infections, although biofilm composition may affect the treatment efficacy. Elsevier 2023-04-15 /pmc/articles/PMC10149328/ /pubmed/37138646 http://dx.doi.org/10.1016/j.bioflm.2023.100123 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Baz, Abdullah Bakri, Ahmed Butcher, Mark Short, Bryn Ghimire, Bhagirath Gaur, Nishtha Jenkins, Toby Short, Robert D. Riggio, Marcello Williams, Craig Ramage, Gordon Brown, Jason L. Staphylococcus aureus strains exhibit heterogenous tolerance to direct cold atmospheric plasma therapy |
title | Staphylococcus aureus strains exhibit heterogenous tolerance to direct cold atmospheric plasma therapy |
title_full | Staphylococcus aureus strains exhibit heterogenous tolerance to direct cold atmospheric plasma therapy |
title_fullStr | Staphylococcus aureus strains exhibit heterogenous tolerance to direct cold atmospheric plasma therapy |
title_full_unstemmed | Staphylococcus aureus strains exhibit heterogenous tolerance to direct cold atmospheric plasma therapy |
title_short | Staphylococcus aureus strains exhibit heterogenous tolerance to direct cold atmospheric plasma therapy |
title_sort | staphylococcus aureus strains exhibit heterogenous tolerance to direct cold atmospheric plasma therapy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10149328/ https://www.ncbi.nlm.nih.gov/pubmed/37138646 http://dx.doi.org/10.1016/j.bioflm.2023.100123 |
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