Efficient detoxication of hydroxylamine and nitrite through heterotrophic nitrification and aerobic denitrification by Acinetobacter johnsonii EN-J1

The co-existence of hydroxylamine (NH(2)OH) and nitrite (NO(2)(–)-N) can aggravate the difficulty of wastewater treatment. The roles of hydroxylamine (NH(2)OH) and nitrite (NO(2)(–)-N) in accelerating the elimination of multiple nitrogen sources by a novel isolated strain of Acinetobacter johnsonii EN-J1 were investigated in this study. The results demonstrated that strain EN-J1 could eliminate 100.00% of NH(2)OH (22.73 mg/L) and 90.09% of NO(2)(–)-N (55.32 mg/L), with maximum consumption rates of 1.22 and 6.75 mg/L/h, respectively. Prominently, the toxic substances NH(2)OH and NO(2)(–)-N could both facilitate nitrogen removal rates. Compared with the control treatment, the elimination rates of nitrate (NO(3)(–)-N) and NO(2)(–)-N were enhanced by 3.44 and 2.36 mg/L/h after supplementation with 10.00 mg/L NH(2)OH, and those of ammonium (NH(4)(+)-N) and NO(3)(–)-N were improved by 0.65 and 1.00 mg/L/h after the addition of 50.00 mg/L NO(2)(–)-N. Furthermore, the nitrogen balance results indicated that over 55.00% of the initial total nitrogen was transformed into gaseous nitrogen by heterotrophic nitrification and aerobic denitrification (HN-AD). Ammonia monooxygenase (AMO), hydroxylamine oxidoreductase (HAO), nitrate reductase (NR), and nitrite reductase (NIR), which are essential for HN-AD, were detected at levels of 0.54, 0.15, 0.14, and 0.01 U/mg protein, respectively. All findings confirmed that strain EN-J1 could efficiently execute HN-AD, detoxify NH(2)OH and NO(2)(–)-N, and ultimately promote nitrogen removal rates.