Cargando…
Performance of two clinical fluorescence imaging systems with different targeted and non-targeted near-infrared fluorophores: a cadaveric explorative study
INTRODUCTION: Near-infrared (NIR) fluorescence-guided surgery is increasingly utilized in humans and pets. As clinical imaging systems are optimized for Indocyanine green (ICG) detection, the usage of targeted dyes necessitates the validation of these systems for each dye. We investigated the impact...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10149874/ https://www.ncbi.nlm.nih.gov/pubmed/37138917 http://dx.doi.org/10.3389/fvets.2023.1091842 |
_version_ | 1785035241121906688 |
---|---|
author | Chiti, Lavinia E. Husi, Benjamin Park, Brian Beer, Patricia D'Orchymont, Faustine Holland, Jason P. Nolff, Mirja C. |
author_facet | Chiti, Lavinia E. Husi, Benjamin Park, Brian Beer, Patricia D'Orchymont, Faustine Holland, Jason P. Nolff, Mirja C. |
author_sort | Chiti, Lavinia E. |
collection | PubMed |
description | INTRODUCTION: Near-infrared (NIR) fluorescence-guided surgery is increasingly utilized in humans and pets. As clinical imaging systems are optimized for Indocyanine green (ICG) detection, the usage of targeted dyes necessitates the validation of these systems for each dye. We investigated the impact of skin pigmentation and tissue overlay on the sensitivity of two NIR cameras (IC-Flow(TM), Visionsense(TM) VS3 Iridum) for the detection of non-targeted (ICG, IRDye800) and targeted (Angiostamp(TM), FAP-Cyan) NIR fluorophores in an ex vivo big animal model. METHODS: We quantitatively measured the limit of detection (LOD) and signal-to-background ratio (SBR) and implemented a semi-quantitative visual score to account for subjective interpretation of images by the surgeon. RESULTS: Visionsense(TM) VS3 Iridum outperformed IC-Flow(TM) in terms of LOD and SBR for the detection of all dyes except FAP-Cyan. Median SBR was negatively affected by skin pigmentation and tissue overlay with both camera systems. Level of agreement between quantitative and semi-quantitative visual score and interobserver agreement were better with Visionsense(TM) VS3 Iridum. CONCLUSION: The overlay of different tissue types and skin pigmentation may negatively affect the ability of the two tested camera systems to identify nanomolar concentrations of targeted-fluorescent dyes and should be considered when planning surgical applications. |
format | Online Article Text |
id | pubmed-10149874 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101498742023-05-02 Performance of two clinical fluorescence imaging systems with different targeted and non-targeted near-infrared fluorophores: a cadaveric explorative study Chiti, Lavinia E. Husi, Benjamin Park, Brian Beer, Patricia D'Orchymont, Faustine Holland, Jason P. Nolff, Mirja C. Front Vet Sci Veterinary Science INTRODUCTION: Near-infrared (NIR) fluorescence-guided surgery is increasingly utilized in humans and pets. As clinical imaging systems are optimized for Indocyanine green (ICG) detection, the usage of targeted dyes necessitates the validation of these systems for each dye. We investigated the impact of skin pigmentation and tissue overlay on the sensitivity of two NIR cameras (IC-Flow(TM), Visionsense(TM) VS3 Iridum) for the detection of non-targeted (ICG, IRDye800) and targeted (Angiostamp(TM), FAP-Cyan) NIR fluorophores in an ex vivo big animal model. METHODS: We quantitatively measured the limit of detection (LOD) and signal-to-background ratio (SBR) and implemented a semi-quantitative visual score to account for subjective interpretation of images by the surgeon. RESULTS: Visionsense(TM) VS3 Iridum outperformed IC-Flow(TM) in terms of LOD and SBR for the detection of all dyes except FAP-Cyan. Median SBR was negatively affected by skin pigmentation and tissue overlay with both camera systems. Level of agreement between quantitative and semi-quantitative visual score and interobserver agreement were better with Visionsense(TM) VS3 Iridum. CONCLUSION: The overlay of different tissue types and skin pigmentation may negatively affect the ability of the two tested camera systems to identify nanomolar concentrations of targeted-fluorescent dyes and should be considered when planning surgical applications. Frontiers Media S.A. 2023-04-17 /pmc/articles/PMC10149874/ /pubmed/37138917 http://dx.doi.org/10.3389/fvets.2023.1091842 Text en Copyright © 2023 Chiti, Husi, Park, Beer, D'Orchymont, Holland and Nolff. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Chiti, Lavinia E. Husi, Benjamin Park, Brian Beer, Patricia D'Orchymont, Faustine Holland, Jason P. Nolff, Mirja C. Performance of two clinical fluorescence imaging systems with different targeted and non-targeted near-infrared fluorophores: a cadaveric explorative study |
title | Performance of two clinical fluorescence imaging systems with different targeted and non-targeted near-infrared fluorophores: a cadaveric explorative study |
title_full | Performance of two clinical fluorescence imaging systems with different targeted and non-targeted near-infrared fluorophores: a cadaveric explorative study |
title_fullStr | Performance of two clinical fluorescence imaging systems with different targeted and non-targeted near-infrared fluorophores: a cadaveric explorative study |
title_full_unstemmed | Performance of two clinical fluorescence imaging systems with different targeted and non-targeted near-infrared fluorophores: a cadaveric explorative study |
title_short | Performance of two clinical fluorescence imaging systems with different targeted and non-targeted near-infrared fluorophores: a cadaveric explorative study |
title_sort | performance of two clinical fluorescence imaging systems with different targeted and non-targeted near-infrared fluorophores: a cadaveric explorative study |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10149874/ https://www.ncbi.nlm.nih.gov/pubmed/37138917 http://dx.doi.org/10.3389/fvets.2023.1091842 |
work_keys_str_mv | AT chitilaviniae performanceoftwoclinicalfluorescenceimagingsystemswithdifferenttargetedandnontargetednearinfraredfluorophoresacadavericexplorativestudy AT husibenjamin performanceoftwoclinicalfluorescenceimagingsystemswithdifferenttargetedandnontargetednearinfraredfluorophoresacadavericexplorativestudy AT parkbrian performanceoftwoclinicalfluorescenceimagingsystemswithdifferenttargetedandnontargetednearinfraredfluorophoresacadavericexplorativestudy AT beerpatricia performanceoftwoclinicalfluorescenceimagingsystemswithdifferenttargetedandnontargetednearinfraredfluorophoresacadavericexplorativestudy AT dorchymontfaustine performanceoftwoclinicalfluorescenceimagingsystemswithdifferenttargetedandnontargetednearinfraredfluorophoresacadavericexplorativestudy AT hollandjasonp performanceoftwoclinicalfluorescenceimagingsystemswithdifferenttargetedandnontargetednearinfraredfluorophoresacadavericexplorativestudy AT nolffmirjac performanceoftwoclinicalfluorescenceimagingsystemswithdifferenttargetedandnontargetednearinfraredfluorophoresacadavericexplorativestudy |