Cargando…
Hypericum japonicum extract inhibited porcine epidemic diarrhea virus in vitro and in vivo
Porcine epidemic diarrhea virus (PEDV) infection causes lethal watery diarrhea and high mortality in neonatal piglets, leading to huge economic losses in the global swine industry. Currently, the existing commercial vaccines cannot fully control PEDV, so it is urgent to develop effective antiviral a...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10149974/ https://www.ncbi.nlm.nih.gov/pubmed/37138845 http://dx.doi.org/10.3389/fphar.2023.1112610 |
_version_ | 1785035263593938944 |
---|---|
author | Rao, Hongyu Su, Weiwei Zhang, Xiaoli Wang, Yonggang Li, Tao Li, Jinliang Zeng, Xuan Li, Peibo |
author_facet | Rao, Hongyu Su, Weiwei Zhang, Xiaoli Wang, Yonggang Li, Tao Li, Jinliang Zeng, Xuan Li, Peibo |
author_sort | Rao, Hongyu |
collection | PubMed |
description | Porcine epidemic diarrhea virus (PEDV) infection causes lethal watery diarrhea and high mortality in neonatal piglets, leading to huge economic losses in the global swine industry. Currently, the existing commercial vaccines cannot fully control PEDV, so it is urgent to develop effective antiviral agents to complement vaccine therapy. In the present study, we investigated the antiviral effect of Hypericum japonicum extract (HJ) against PEDV in vivo and in vitro. In in vitro assays, HJ could directly inactivate PEDV strains; moreover, it inhibited the proliferation of PEDV strains in Vero or IPI-FX cells at its non-cytotoxic concentrations. Time of addition assays revealed that HJ mainly inhibited PEDV at the later stages of the viral life cycle. In in vivo, compared with the model group, HJ could reduce the viral titers in the intestines of infected piglets, and improve their intestinal pathological, indicating that HJ could protect the newborn piglets from highly pathogenic PEDV variant infection. Furthermore, this effect may be related to the fact that HJ can not only directly inhibit viruses, but also regulate the structure of intestinal microbiota. In conclusion, our results indicate that Hypericum japonicum could inhibit PEDV replication in vitro and in vivo and might possess the potential to develop as the anti-PEDV drug. |
format | Online Article Text |
id | pubmed-10149974 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101499742023-05-02 Hypericum japonicum extract inhibited porcine epidemic diarrhea virus in vitro and in vivo Rao, Hongyu Su, Weiwei Zhang, Xiaoli Wang, Yonggang Li, Tao Li, Jinliang Zeng, Xuan Li, Peibo Front Pharmacol Pharmacology Porcine epidemic diarrhea virus (PEDV) infection causes lethal watery diarrhea and high mortality in neonatal piglets, leading to huge economic losses in the global swine industry. Currently, the existing commercial vaccines cannot fully control PEDV, so it is urgent to develop effective antiviral agents to complement vaccine therapy. In the present study, we investigated the antiviral effect of Hypericum japonicum extract (HJ) against PEDV in vivo and in vitro. In in vitro assays, HJ could directly inactivate PEDV strains; moreover, it inhibited the proliferation of PEDV strains in Vero or IPI-FX cells at its non-cytotoxic concentrations. Time of addition assays revealed that HJ mainly inhibited PEDV at the later stages of the viral life cycle. In in vivo, compared with the model group, HJ could reduce the viral titers in the intestines of infected piglets, and improve their intestinal pathological, indicating that HJ could protect the newborn piglets from highly pathogenic PEDV variant infection. Furthermore, this effect may be related to the fact that HJ can not only directly inhibit viruses, but also regulate the structure of intestinal microbiota. In conclusion, our results indicate that Hypericum japonicum could inhibit PEDV replication in vitro and in vivo and might possess the potential to develop as the anti-PEDV drug. Frontiers Media S.A. 2023-04-17 /pmc/articles/PMC10149974/ /pubmed/37138845 http://dx.doi.org/10.3389/fphar.2023.1112610 Text en Copyright © 2023 Rao, Su, Zhang, Wang, Li, Li, Zeng and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Rao, Hongyu Su, Weiwei Zhang, Xiaoli Wang, Yonggang Li, Tao Li, Jinliang Zeng, Xuan Li, Peibo Hypericum japonicum extract inhibited porcine epidemic diarrhea virus in vitro and in vivo |
title |
Hypericum japonicum extract inhibited porcine epidemic diarrhea virus in vitro and in vivo
|
title_full |
Hypericum japonicum extract inhibited porcine epidemic diarrhea virus in vitro and in vivo
|
title_fullStr |
Hypericum japonicum extract inhibited porcine epidemic diarrhea virus in vitro and in vivo
|
title_full_unstemmed |
Hypericum japonicum extract inhibited porcine epidemic diarrhea virus in vitro and in vivo
|
title_short |
Hypericum japonicum extract inhibited porcine epidemic diarrhea virus in vitro and in vivo
|
title_sort | hypericum japonicum extract inhibited porcine epidemic diarrhea virus in vitro and in vivo |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10149974/ https://www.ncbi.nlm.nih.gov/pubmed/37138845 http://dx.doi.org/10.3389/fphar.2023.1112610 |
work_keys_str_mv | AT raohongyu hypericumjaponicumextractinhibitedporcineepidemicdiarrheavirusinvitroandinvivo AT suweiwei hypericumjaponicumextractinhibitedporcineepidemicdiarrheavirusinvitroandinvivo AT zhangxiaoli hypericumjaponicumextractinhibitedporcineepidemicdiarrheavirusinvitroandinvivo AT wangyonggang hypericumjaponicumextractinhibitedporcineepidemicdiarrheavirusinvitroandinvivo AT litao hypericumjaponicumextractinhibitedporcineepidemicdiarrheavirusinvitroandinvivo AT lijinliang hypericumjaponicumextractinhibitedporcineepidemicdiarrheavirusinvitroandinvivo AT zengxuan hypericumjaponicumextractinhibitedporcineepidemicdiarrheavirusinvitroandinvivo AT lipeibo hypericumjaponicumextractinhibitedporcineepidemicdiarrheavirusinvitroandinvivo |