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Whole-brain imaging of freely-moving zebrafish

One of the holy grails of neuroscience is to record the activity of every neuron in the brain while an animal moves freely and performs complex behavioral tasks. While important steps forward have been taken recently in large-scale neural recording in rodent models, single neuron resolution across t...

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Autores principales: Hasani, Hamid, Sun, Jipeng, Zhu, Shuyu I., Rong, Qiangzhou, Willomitzer, Florian, Amor, Rumelo, McConnell, Gail, Cossairt, Oliver, Goodhill, Geoffrey J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10150962/
https://www.ncbi.nlm.nih.gov/pubmed/37139528
http://dx.doi.org/10.3389/fnins.2023.1127574
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author Hasani, Hamid
Sun, Jipeng
Zhu, Shuyu I.
Rong, Qiangzhou
Willomitzer, Florian
Amor, Rumelo
McConnell, Gail
Cossairt, Oliver
Goodhill, Geoffrey J.
author_facet Hasani, Hamid
Sun, Jipeng
Zhu, Shuyu I.
Rong, Qiangzhou
Willomitzer, Florian
Amor, Rumelo
McConnell, Gail
Cossairt, Oliver
Goodhill, Geoffrey J.
author_sort Hasani, Hamid
collection PubMed
description One of the holy grails of neuroscience is to record the activity of every neuron in the brain while an animal moves freely and performs complex behavioral tasks. While important steps forward have been taken recently in large-scale neural recording in rodent models, single neuron resolution across the entire mammalian brain remains elusive. In contrast the larval zebrafish offers great promise in this regard. Zebrafish are a vertebrate model with substantial homology to the mammalian brain, but their transparency allows whole-brain recordings of genetically-encoded fluorescent indicators at single-neuron resolution using optical microscopy techniques. Furthermore zebrafish begin to show a complex repertoire of natural behavior from an early age, including hunting small, fast-moving prey using visual cues. Until recently work to address the neural bases of these behaviors mostly relied on assays where the fish was immobilized under the microscope objective, and stimuli such as prey were presented virtually. However significant progress has recently been made in developing brain imaging techniques for zebrafish which are not immobilized. Here we discuss recent advances, focusing particularly on techniques based on light-field microscopy. We also draw attention to several important outstanding issues which remain to be addressed to increase the ecological validity of the results obtained.
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spelling pubmed-101509622023-05-02 Whole-brain imaging of freely-moving zebrafish Hasani, Hamid Sun, Jipeng Zhu, Shuyu I. Rong, Qiangzhou Willomitzer, Florian Amor, Rumelo McConnell, Gail Cossairt, Oliver Goodhill, Geoffrey J. Front Neurosci Neuroscience One of the holy grails of neuroscience is to record the activity of every neuron in the brain while an animal moves freely and performs complex behavioral tasks. While important steps forward have been taken recently in large-scale neural recording in rodent models, single neuron resolution across the entire mammalian brain remains elusive. In contrast the larval zebrafish offers great promise in this regard. Zebrafish are a vertebrate model with substantial homology to the mammalian brain, but their transparency allows whole-brain recordings of genetically-encoded fluorescent indicators at single-neuron resolution using optical microscopy techniques. Furthermore zebrafish begin to show a complex repertoire of natural behavior from an early age, including hunting small, fast-moving prey using visual cues. Until recently work to address the neural bases of these behaviors mostly relied on assays where the fish was immobilized under the microscope objective, and stimuli such as prey were presented virtually. However significant progress has recently been made in developing brain imaging techniques for zebrafish which are not immobilized. Here we discuss recent advances, focusing particularly on techniques based on light-field microscopy. We also draw attention to several important outstanding issues which remain to be addressed to increase the ecological validity of the results obtained. Frontiers Media S.A. 2023-04-17 /pmc/articles/PMC10150962/ /pubmed/37139528 http://dx.doi.org/10.3389/fnins.2023.1127574 Text en Copyright © 2023 Hasani, Sun, Zhu, Rong, Willomitzer, Amor, McConnell, Cossairt and Goodhill. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Hasani, Hamid
Sun, Jipeng
Zhu, Shuyu I.
Rong, Qiangzhou
Willomitzer, Florian
Amor, Rumelo
McConnell, Gail
Cossairt, Oliver
Goodhill, Geoffrey J.
Whole-brain imaging of freely-moving zebrafish
title Whole-brain imaging of freely-moving zebrafish
title_full Whole-brain imaging of freely-moving zebrafish
title_fullStr Whole-brain imaging of freely-moving zebrafish
title_full_unstemmed Whole-brain imaging of freely-moving zebrafish
title_short Whole-brain imaging of freely-moving zebrafish
title_sort whole-brain imaging of freely-moving zebrafish
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10150962/
https://www.ncbi.nlm.nih.gov/pubmed/37139528
http://dx.doi.org/10.3389/fnins.2023.1127574
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