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Lycium barbarum polysaccharide alleviates dextran sodium sulfate-induced inflammatory bowel disease by regulating M1/M2 macrophage polarization via the STAT1 and STAT6 pathways

Disruption of colonic homeostasis caused by aberrant M1/M2 macrophage polarization contributes to the development of inflammatory bowel disease (IBD). Lycium barbarum polysaccharide (LBP) is the primary active constituent of traditional Chinese herbal Lycium barbarum L., which has been widely demons...

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Autores principales: Wang, Juan, Gao, Huiying, Xie, Yuan, Wang, Peng, Li, Yu, Zhao, Junli, Wang, Chunlin, Ma, Xin, Wang, Yuwen, Mao, Qinwen, Xia, Haibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10151498/
https://www.ncbi.nlm.nih.gov/pubmed/37144216
http://dx.doi.org/10.3389/fphar.2023.1044576
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author Wang, Juan
Gao, Huiying
Xie, Yuan
Wang, Peng
Li, Yu
Zhao, Junli
Wang, Chunlin
Ma, Xin
Wang, Yuwen
Mao, Qinwen
Xia, Haibin
author_facet Wang, Juan
Gao, Huiying
Xie, Yuan
Wang, Peng
Li, Yu
Zhao, Junli
Wang, Chunlin
Ma, Xin
Wang, Yuwen
Mao, Qinwen
Xia, Haibin
author_sort Wang, Juan
collection PubMed
description Disruption of colonic homeostasis caused by aberrant M1/M2 macrophage polarization contributes to the development of inflammatory bowel disease (IBD). Lycium barbarum polysaccharide (LBP) is the primary active constituent of traditional Chinese herbal Lycium barbarum L., which has been widely demonstrated to have important functions in regulating immune activity and anti-inflammatory. Thus, LBP may protect against IBD. To test this hypothesis, the DSS-induced colitis model was established in mice, then the mice were treated with LBP. The results indicated that LBP attenuated the weight loss, colon shortening, disease activity index (DAI), and histopathological scores of colon tissues in colitis mice, suggesting that LBP could protect against IBD. Besides, LBP decreased the number of M1 macrophages and the protein level of Nitric oxide synthase 2(NOS2) as a marker of M1 macrophages and enhanced the number of M2 macrophages and the protein level of Arginase 1(Arg-1) as a marker of M2 macrophages in colon tissues from mice with colitis, suggesting that LBP may protect against IBD by regulating macrophage polarization. Next, the mechanistic studies in RAW264.7 cells showed that LBP inhibited M1-like phenotype by inhibiting the phosphorylation of STAT1, and promoted M2-like phenotype by promoting the phosphorylation of STAT6. Finally, immunofluorescence double-staining results of colon tissues showed that LBP regulated STAT1 and STAT6 pathways in vivo. The results in the study demonstrated that LBP could protect against IBD by regulating macrophage polarization through the STAT1 and STAT6 pathways.
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spelling pubmed-101514982023-05-03 Lycium barbarum polysaccharide alleviates dextran sodium sulfate-induced inflammatory bowel disease by regulating M1/M2 macrophage polarization via the STAT1 and STAT6 pathways Wang, Juan Gao, Huiying Xie, Yuan Wang, Peng Li, Yu Zhao, Junli Wang, Chunlin Ma, Xin Wang, Yuwen Mao, Qinwen Xia, Haibin Front Pharmacol Pharmacology Disruption of colonic homeostasis caused by aberrant M1/M2 macrophage polarization contributes to the development of inflammatory bowel disease (IBD). Lycium barbarum polysaccharide (LBP) is the primary active constituent of traditional Chinese herbal Lycium barbarum L., which has been widely demonstrated to have important functions in regulating immune activity and anti-inflammatory. Thus, LBP may protect against IBD. To test this hypothesis, the DSS-induced colitis model was established in mice, then the mice were treated with LBP. The results indicated that LBP attenuated the weight loss, colon shortening, disease activity index (DAI), and histopathological scores of colon tissues in colitis mice, suggesting that LBP could protect against IBD. Besides, LBP decreased the number of M1 macrophages and the protein level of Nitric oxide synthase 2(NOS2) as a marker of M1 macrophages and enhanced the number of M2 macrophages and the protein level of Arginase 1(Arg-1) as a marker of M2 macrophages in colon tissues from mice with colitis, suggesting that LBP may protect against IBD by regulating macrophage polarization. Next, the mechanistic studies in RAW264.7 cells showed that LBP inhibited M1-like phenotype by inhibiting the phosphorylation of STAT1, and promoted M2-like phenotype by promoting the phosphorylation of STAT6. Finally, immunofluorescence double-staining results of colon tissues showed that LBP regulated STAT1 and STAT6 pathways in vivo. The results in the study demonstrated that LBP could protect against IBD by regulating macrophage polarization through the STAT1 and STAT6 pathways. Frontiers Media S.A. 2023-04-18 /pmc/articles/PMC10151498/ /pubmed/37144216 http://dx.doi.org/10.3389/fphar.2023.1044576 Text en Copyright © 2023 Wang, Gao, Xie, Wang, Li, Zhao, Wang, Ma, Wang, Mao and Xia. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Wang, Juan
Gao, Huiying
Xie, Yuan
Wang, Peng
Li, Yu
Zhao, Junli
Wang, Chunlin
Ma, Xin
Wang, Yuwen
Mao, Qinwen
Xia, Haibin
Lycium barbarum polysaccharide alleviates dextran sodium sulfate-induced inflammatory bowel disease by regulating M1/M2 macrophage polarization via the STAT1 and STAT6 pathways
title Lycium barbarum polysaccharide alleviates dextran sodium sulfate-induced inflammatory bowel disease by regulating M1/M2 macrophage polarization via the STAT1 and STAT6 pathways
title_full Lycium barbarum polysaccharide alleviates dextran sodium sulfate-induced inflammatory bowel disease by regulating M1/M2 macrophage polarization via the STAT1 and STAT6 pathways
title_fullStr Lycium barbarum polysaccharide alleviates dextran sodium sulfate-induced inflammatory bowel disease by regulating M1/M2 macrophage polarization via the STAT1 and STAT6 pathways
title_full_unstemmed Lycium barbarum polysaccharide alleviates dextran sodium sulfate-induced inflammatory bowel disease by regulating M1/M2 macrophage polarization via the STAT1 and STAT6 pathways
title_short Lycium barbarum polysaccharide alleviates dextran sodium sulfate-induced inflammatory bowel disease by regulating M1/M2 macrophage polarization via the STAT1 and STAT6 pathways
title_sort lycium barbarum polysaccharide alleviates dextran sodium sulfate-induced inflammatory bowel disease by regulating m1/m2 macrophage polarization via the stat1 and stat6 pathways
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10151498/
https://www.ncbi.nlm.nih.gov/pubmed/37144216
http://dx.doi.org/10.3389/fphar.2023.1044576
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