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Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review
Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer death worldwide. Immunotherapy with immune checkpoint inhibitors (ICI) has significantly improved outcomes in some patients, however 80-85% of patients receiving immunotherapy develop primary resistance, manifesting as a lack...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10151669/ https://www.ncbi.nlm.nih.gov/pubmed/37143952 http://dx.doi.org/10.3389/fonc.2023.1129195 |
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author | Rangamuwa, Kanishka Aloe, Christian Christie, Michael Asselin-Labat, Marie-Liesse Batey, Daniel Irving, Lou John, Thomas Bozinovski, Steven Leong, Tracy L. Steinfort, Daniel |
author_facet | Rangamuwa, Kanishka Aloe, Christian Christie, Michael Asselin-Labat, Marie-Liesse Batey, Daniel Irving, Lou John, Thomas Bozinovski, Steven Leong, Tracy L. Steinfort, Daniel |
author_sort | Rangamuwa, Kanishka |
collection | PubMed |
description | Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer death worldwide. Immunotherapy with immune checkpoint inhibitors (ICI) has significantly improved outcomes in some patients, however 80-85% of patients receiving immunotherapy develop primary resistance, manifesting as a lack of response to therapy. Of those that do have an initial response, disease progression may occur due to acquired resistance. The make-up of the tumour microenvironment (TME) and the interaction between tumour infiltrating immune cells and cancer cells can have a large impact on the response to immunotherapy. Robust assessment of the TME with accurate and reproducible methods is vital to understanding mechanisms of immunotherapy resistance. In this paper we will review the evidence of several methodologies to assess the TME, including multiplex immunohistochemistry, imaging mass cytometry, flow cytometry, mass cytometry and RNA sequencing. |
format | Online Article Text |
id | pubmed-10151669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101516692023-05-03 Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review Rangamuwa, Kanishka Aloe, Christian Christie, Michael Asselin-Labat, Marie-Liesse Batey, Daniel Irving, Lou John, Thomas Bozinovski, Steven Leong, Tracy L. Steinfort, Daniel Front Oncol Oncology Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer death worldwide. Immunotherapy with immune checkpoint inhibitors (ICI) has significantly improved outcomes in some patients, however 80-85% of patients receiving immunotherapy develop primary resistance, manifesting as a lack of response to therapy. Of those that do have an initial response, disease progression may occur due to acquired resistance. The make-up of the tumour microenvironment (TME) and the interaction between tumour infiltrating immune cells and cancer cells can have a large impact on the response to immunotherapy. Robust assessment of the TME with accurate and reproducible methods is vital to understanding mechanisms of immunotherapy resistance. In this paper we will review the evidence of several methodologies to assess the TME, including multiplex immunohistochemistry, imaging mass cytometry, flow cytometry, mass cytometry and RNA sequencing. Frontiers Media S.A. 2023-04-18 /pmc/articles/PMC10151669/ /pubmed/37143952 http://dx.doi.org/10.3389/fonc.2023.1129195 Text en Copyright © 2023 Rangamuwa, Aloe, Christie, Asselin-Labat, Batey, Irving, John, Bozinovski, Leong and Steinfort https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Rangamuwa, Kanishka Aloe, Christian Christie, Michael Asselin-Labat, Marie-Liesse Batey, Daniel Irving, Lou John, Thomas Bozinovski, Steven Leong, Tracy L. Steinfort, Daniel Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review |
title | Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review |
title_full | Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review |
title_fullStr | Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review |
title_full_unstemmed | Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review |
title_short | Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review |
title_sort | methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. a narrative review |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10151669/ https://www.ncbi.nlm.nih.gov/pubmed/37143952 http://dx.doi.org/10.3389/fonc.2023.1129195 |
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