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Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review

Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer death worldwide. Immunotherapy with immune checkpoint inhibitors (ICI) has significantly improved outcomes in some patients, however 80-85% of patients receiving immunotherapy develop primary resistance, manifesting as a lack...

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Autores principales: Rangamuwa, Kanishka, Aloe, Christian, Christie, Michael, Asselin-Labat, Marie-Liesse, Batey, Daniel, Irving, Lou, John, Thomas, Bozinovski, Steven, Leong, Tracy L., Steinfort, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10151669/
https://www.ncbi.nlm.nih.gov/pubmed/37143952
http://dx.doi.org/10.3389/fonc.2023.1129195
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author Rangamuwa, Kanishka
Aloe, Christian
Christie, Michael
Asselin-Labat, Marie-Liesse
Batey, Daniel
Irving, Lou
John, Thomas
Bozinovski, Steven
Leong, Tracy L.
Steinfort, Daniel
author_facet Rangamuwa, Kanishka
Aloe, Christian
Christie, Michael
Asselin-Labat, Marie-Liesse
Batey, Daniel
Irving, Lou
John, Thomas
Bozinovski, Steven
Leong, Tracy L.
Steinfort, Daniel
author_sort Rangamuwa, Kanishka
collection PubMed
description Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer death worldwide. Immunotherapy with immune checkpoint inhibitors (ICI) has significantly improved outcomes in some patients, however 80-85% of patients receiving immunotherapy develop primary resistance, manifesting as a lack of response to therapy. Of those that do have an initial response, disease progression may occur due to acquired resistance. The make-up of the tumour microenvironment (TME) and the interaction between tumour infiltrating immune cells and cancer cells can have a large impact on the response to immunotherapy. Robust assessment of the TME with accurate and reproducible methods is vital to understanding mechanisms of immunotherapy resistance. In this paper we will review the evidence of several methodologies to assess the TME, including multiplex immunohistochemistry, imaging mass cytometry, flow cytometry, mass cytometry and RNA sequencing.
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spelling pubmed-101516692023-05-03 Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review Rangamuwa, Kanishka Aloe, Christian Christie, Michael Asselin-Labat, Marie-Liesse Batey, Daniel Irving, Lou John, Thomas Bozinovski, Steven Leong, Tracy L. Steinfort, Daniel Front Oncol Oncology Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer death worldwide. Immunotherapy with immune checkpoint inhibitors (ICI) has significantly improved outcomes in some patients, however 80-85% of patients receiving immunotherapy develop primary resistance, manifesting as a lack of response to therapy. Of those that do have an initial response, disease progression may occur due to acquired resistance. The make-up of the tumour microenvironment (TME) and the interaction between tumour infiltrating immune cells and cancer cells can have a large impact on the response to immunotherapy. Robust assessment of the TME with accurate and reproducible methods is vital to understanding mechanisms of immunotherapy resistance. In this paper we will review the evidence of several methodologies to assess the TME, including multiplex immunohistochemistry, imaging mass cytometry, flow cytometry, mass cytometry and RNA sequencing. Frontiers Media S.A. 2023-04-18 /pmc/articles/PMC10151669/ /pubmed/37143952 http://dx.doi.org/10.3389/fonc.2023.1129195 Text en Copyright © 2023 Rangamuwa, Aloe, Christie, Asselin-Labat, Batey, Irving, John, Bozinovski, Leong and Steinfort https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Rangamuwa, Kanishka
Aloe, Christian
Christie, Michael
Asselin-Labat, Marie-Liesse
Batey, Daniel
Irving, Lou
John, Thomas
Bozinovski, Steven
Leong, Tracy L.
Steinfort, Daniel
Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review
title Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review
title_full Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review
title_fullStr Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review
title_full_unstemmed Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review
title_short Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review
title_sort methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. a narrative review
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10151669/
https://www.ncbi.nlm.nih.gov/pubmed/37143952
http://dx.doi.org/10.3389/fonc.2023.1129195
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