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LC–MS/MS-based multiplex antibacterial platform for therapeutic drug monitoring in intensive care unit patients
Empirically prescribed standard dosing regimens of antibacterial agents may result in insufficient or excess plasma concentrations with persistently poor clinical outcomes, especially for patients in intensive care units (ICUs). Therapeutic drug monitoring (TDM) of antibacterial agents can guide dos...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10151781/ https://www.ncbi.nlm.nih.gov/pubmed/37144212 http://dx.doi.org/10.3389/fphar.2023.1116071 |
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author | Liu, Liang Zhang, Liu Zheng, Xiangyi Liu, Xing Liu, Wei Wu, Jianhua |
author_facet | Liu, Liang Zhang, Liu Zheng, Xiangyi Liu, Xing Liu, Wei Wu, Jianhua |
author_sort | Liu, Liang |
collection | PubMed |
description | Empirically prescribed standard dosing regimens of antibacterial agents may result in insufficient or excess plasma concentrations with persistently poor clinical outcomes, especially for patients in intensive care units (ICUs). Therapeutic drug monitoring (TDM) of antibacterial agents can guide dose adjustments to benefit patients. In this study, we developed a robust, sensitive, and simple liquid chromatography-tandem mass spectrometry (LC–MS/MS) platform for the quantification of 14 antibacterial and antifungal agents (beta-lactams piperacillin, cefoperazone, and meropenem; beta-lactamase inhibitors tazobactam and sulbactam; antifungal agents fluconazole, caspofungin, posaconazole, and voriconazole; and daptomycin, vancomycin, teicoplanin, linezolid, and tigecycline) that can be used for patients with severe infection. This assay requires only 100 µL of serum with rapid protein precipitation. Chromatographic analysis was performed using a Waters Acquity UPLC C8 column. Three stable isotope-labeled antibacterial agents and one analogue were used as internal standards. Calibration curves ranged from 0.1–100 μg/mL, 0.1–50 μg/mL, and 0.3–100 μg/mL for different drugs, and all correlation coefficients were greater than 0.9085. Intra- and inter-day imprecision and inaccuracy values were below 15%. After validation, this new method was successfully employed for TDM in routine practice. |
format | Online Article Text |
id | pubmed-10151781 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101517812023-05-03 LC–MS/MS-based multiplex antibacterial platform for therapeutic drug monitoring in intensive care unit patients Liu, Liang Zhang, Liu Zheng, Xiangyi Liu, Xing Liu, Wei Wu, Jianhua Front Pharmacol Pharmacology Empirically prescribed standard dosing regimens of antibacterial agents may result in insufficient or excess plasma concentrations with persistently poor clinical outcomes, especially for patients in intensive care units (ICUs). Therapeutic drug monitoring (TDM) of antibacterial agents can guide dose adjustments to benefit patients. In this study, we developed a robust, sensitive, and simple liquid chromatography-tandem mass spectrometry (LC–MS/MS) platform for the quantification of 14 antibacterial and antifungal agents (beta-lactams piperacillin, cefoperazone, and meropenem; beta-lactamase inhibitors tazobactam and sulbactam; antifungal agents fluconazole, caspofungin, posaconazole, and voriconazole; and daptomycin, vancomycin, teicoplanin, linezolid, and tigecycline) that can be used for patients with severe infection. This assay requires only 100 µL of serum with rapid protein precipitation. Chromatographic analysis was performed using a Waters Acquity UPLC C8 column. Three stable isotope-labeled antibacterial agents and one analogue were used as internal standards. Calibration curves ranged from 0.1–100 μg/mL, 0.1–50 μg/mL, and 0.3–100 μg/mL for different drugs, and all correlation coefficients were greater than 0.9085. Intra- and inter-day imprecision and inaccuracy values were below 15%. After validation, this new method was successfully employed for TDM in routine practice. Frontiers Media S.A. 2023-04-18 /pmc/articles/PMC10151781/ /pubmed/37144212 http://dx.doi.org/10.3389/fphar.2023.1116071 Text en Copyright © 2023 Liu, Zhang, Zheng, Liu, Liu and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Liu, Liang Zhang, Liu Zheng, Xiangyi Liu, Xing Liu, Wei Wu, Jianhua LC–MS/MS-based multiplex antibacterial platform for therapeutic drug monitoring in intensive care unit patients |
title | LC–MS/MS-based multiplex antibacterial platform for therapeutic drug monitoring in intensive care unit patients |
title_full | LC–MS/MS-based multiplex antibacterial platform for therapeutic drug monitoring in intensive care unit patients |
title_fullStr | LC–MS/MS-based multiplex antibacterial platform for therapeutic drug monitoring in intensive care unit patients |
title_full_unstemmed | LC–MS/MS-based multiplex antibacterial platform for therapeutic drug monitoring in intensive care unit patients |
title_short | LC–MS/MS-based multiplex antibacterial platform for therapeutic drug monitoring in intensive care unit patients |
title_sort | lc–ms/ms-based multiplex antibacterial platform for therapeutic drug monitoring in intensive care unit patients |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10151781/ https://www.ncbi.nlm.nih.gov/pubmed/37144212 http://dx.doi.org/10.3389/fphar.2023.1116071 |
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