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Anti-inflammatory Activity of Tanshinone-Related Diterpenes from Perovskia artemisioides Roots

[Image: see text] Perovskia artemisioides is a perennial and aromatic plant widely distributed in the Baluchestan region of Iran. Phytochemical analysis of a n-hexane extract of P. artemisioides roots, guided by an analytical approach based on LC-ESI/LTQOrbitrap/MS/MS, yielded six previously undescr...

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Autores principales: Sadeghi, Zahra, Cerulli, Antonietta, Marzocco, Stefania, Moridi Farimani, Mahdi, Masullo, Milena, Piacente, Sonia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society and American Society of Pharmacognosy 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10152488/
https://www.ncbi.nlm.nih.gov/pubmed/37040078
http://dx.doi.org/10.1021/acs.jnatprod.2c01004
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author Sadeghi, Zahra
Cerulli, Antonietta
Marzocco, Stefania
Moridi Farimani, Mahdi
Masullo, Milena
Piacente, Sonia
author_facet Sadeghi, Zahra
Cerulli, Antonietta
Marzocco, Stefania
Moridi Farimani, Mahdi
Masullo, Milena
Piacente, Sonia
author_sort Sadeghi, Zahra
collection PubMed
description [Image: see text] Perovskia artemisioides is a perennial and aromatic plant widely distributed in the Baluchestan region of Iran. Phytochemical analysis of a n-hexane extract of P. artemisioides roots, guided by an analytical approach based on LC-ESI/LTQOrbitrap/MS/MS, yielded six previously undescribed diterpenoid compounds (2, 9–11, 16, and 20), and 19 known diterpenoids, for which the structures were elucidated by 1D and 2D NMR experiments. Some of the isolated compounds showed significant anti-inflammatory activity using J774A.1 macrophage cells stimulated with Escherichia coli lipopolysaccharide. In particular, compounds 6, 8, 17, 18, 20, and 22 significantly inhibited the release of nitric oxide and the expression of related pro-inflammatory enzymes, such as inducible nitric oxide synthase and cycloxygenase-2. Moreover, two compounds that showed the highest activity in reducing nitric oxide release (6 and 18) were tested to evaluate their effects on nitrotyrosine formation and reactive oxygen species release. Both compounds inhibited ROS release and, in particular, compound 6 also inhibited nitrotyrosine formation at all tested concentrations, thus indicating a significant antioxidant potential.
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spelling pubmed-101524882023-05-03 Anti-inflammatory Activity of Tanshinone-Related Diterpenes from Perovskia artemisioides Roots Sadeghi, Zahra Cerulli, Antonietta Marzocco, Stefania Moridi Farimani, Mahdi Masullo, Milena Piacente, Sonia J Nat Prod [Image: see text] Perovskia artemisioides is a perennial and aromatic plant widely distributed in the Baluchestan region of Iran. Phytochemical analysis of a n-hexane extract of P. artemisioides roots, guided by an analytical approach based on LC-ESI/LTQOrbitrap/MS/MS, yielded six previously undescribed diterpenoid compounds (2, 9–11, 16, and 20), and 19 known diterpenoids, for which the structures were elucidated by 1D and 2D NMR experiments. Some of the isolated compounds showed significant anti-inflammatory activity using J774A.1 macrophage cells stimulated with Escherichia coli lipopolysaccharide. In particular, compounds 6, 8, 17, 18, 20, and 22 significantly inhibited the release of nitric oxide and the expression of related pro-inflammatory enzymes, such as inducible nitric oxide synthase and cycloxygenase-2. Moreover, two compounds that showed the highest activity in reducing nitric oxide release (6 and 18) were tested to evaluate their effects on nitrotyrosine formation and reactive oxygen species release. Both compounds inhibited ROS release and, in particular, compound 6 also inhibited nitrotyrosine formation at all tested concentrations, thus indicating a significant antioxidant potential. American Chemical Society and American Society of Pharmacognosy 2023-04-11 /pmc/articles/PMC10152488/ /pubmed/37040078 http://dx.doi.org/10.1021/acs.jnatprod.2c01004 Text en © 2023 The Authors. Published by American Chemical Society and American Society of Pharmacognosy https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Sadeghi, Zahra
Cerulli, Antonietta
Marzocco, Stefania
Moridi Farimani, Mahdi
Masullo, Milena
Piacente, Sonia
Anti-inflammatory Activity of Tanshinone-Related Diterpenes from Perovskia artemisioides Roots
title Anti-inflammatory Activity of Tanshinone-Related Diterpenes from Perovskia artemisioides Roots
title_full Anti-inflammatory Activity of Tanshinone-Related Diterpenes from Perovskia artemisioides Roots
title_fullStr Anti-inflammatory Activity of Tanshinone-Related Diterpenes from Perovskia artemisioides Roots
title_full_unstemmed Anti-inflammatory Activity of Tanshinone-Related Diterpenes from Perovskia artemisioides Roots
title_short Anti-inflammatory Activity of Tanshinone-Related Diterpenes from Perovskia artemisioides Roots
title_sort anti-inflammatory activity of tanshinone-related diterpenes from perovskia artemisioides roots
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10152488/
https://www.ncbi.nlm.nih.gov/pubmed/37040078
http://dx.doi.org/10.1021/acs.jnatprod.2c01004
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