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Molecular mechanism of engineered Zymomonas mobilis to furfural and acetic acid stress

Acetic acid and furfural (AF) are two major inhibitors of microorganisms during lignocellulosic ethanol production. In our previous study, we successfully engineered Zymomonas mobilis 532 (ZM532) strain by genome shuffling, but the molecular mechanisms of tolerance to inhibitors were still unknown....

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Autores principales: Shabbir, Samina, Wang, Weiting, Nawaz, Mohsin, Boruah, Prerona, Kulyar, Muhammad Fakhar-e-Alam, Chen, Mao, Wu, Bo, Liu, Panting, Dai, Yonghua, Sun, Lingling, Gou, Qiyu, Liu, Renbin, Hu, Guoquan, Younis, Tahira, He, Mingxiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10152622/
https://www.ncbi.nlm.nih.gov/pubmed/37127628
http://dx.doi.org/10.1186/s12934-023-02095-1
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author Shabbir, Samina
Wang, Weiting
Nawaz, Mohsin
Boruah, Prerona
Kulyar, Muhammad Fakhar-e-Alam
Chen, Mao
Wu, Bo
Liu, Panting
Dai, Yonghua
Sun, Lingling
Gou, Qiyu
Liu, Renbin
Hu, Guoquan
Younis, Tahira
He, Mingxiong
author_facet Shabbir, Samina
Wang, Weiting
Nawaz, Mohsin
Boruah, Prerona
Kulyar, Muhammad Fakhar-e-Alam
Chen, Mao
Wu, Bo
Liu, Panting
Dai, Yonghua
Sun, Lingling
Gou, Qiyu
Liu, Renbin
Hu, Guoquan
Younis, Tahira
He, Mingxiong
author_sort Shabbir, Samina
collection PubMed
description Acetic acid and furfural (AF) are two major inhibitors of microorganisms during lignocellulosic ethanol production. In our previous study, we successfully engineered Zymomonas mobilis 532 (ZM532) strain by genome shuffling, but the molecular mechanisms of tolerance to inhibitors were still unknown. Therefore, this study investigated the responses of ZM532 and its wild-type Z. mobilis (ZM4) to AF using multi-omics approaches (transcriptomics, genomics, and label free quantitative proteomics). Based on RNA-Seq data, two differentially expressed genes, ZMO_RS02740 (up-regulated) and ZMO_RS06525 (down-regulated) were knocked out and over-expressed through CRISPR-Cas technology to investigate their roles in AF tolerance. Overall, we identified 1865 and 14 novel DEGs in ZM532 and wild-type ZM4. In contrast, 1532 proteins were identified in ZM532 and wild-type ZM4. Among these, we found 96 important genes in ZM532 involving acid resistance mechanisms and survival rates against stressors. Furthermore, our knockout results demonstrated that growth activity and glucose consumption of mutant strains ZM532∆ZMO_RS02740 and ZM4∆ZMO_RS02740 decreased with increased fermentation time from 42 to 55 h and ethanol production up to 58% in ZM532 than that in ZM532∆ZMO_RS02740. Hence, these findings suggest ZMO_RS02740 as a protective strategy for ZM ethanol production under stressful conditions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12934-023-02095-1.
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spelling pubmed-101526222023-05-03 Molecular mechanism of engineered Zymomonas mobilis to furfural and acetic acid stress Shabbir, Samina Wang, Weiting Nawaz, Mohsin Boruah, Prerona Kulyar, Muhammad Fakhar-e-Alam Chen, Mao Wu, Bo Liu, Panting Dai, Yonghua Sun, Lingling Gou, Qiyu Liu, Renbin Hu, Guoquan Younis, Tahira He, Mingxiong Microb Cell Fact Research Acetic acid and furfural (AF) are two major inhibitors of microorganisms during lignocellulosic ethanol production. In our previous study, we successfully engineered Zymomonas mobilis 532 (ZM532) strain by genome shuffling, but the molecular mechanisms of tolerance to inhibitors were still unknown. Therefore, this study investigated the responses of ZM532 and its wild-type Z. mobilis (ZM4) to AF using multi-omics approaches (transcriptomics, genomics, and label free quantitative proteomics). Based on RNA-Seq data, two differentially expressed genes, ZMO_RS02740 (up-regulated) and ZMO_RS06525 (down-regulated) were knocked out and over-expressed through CRISPR-Cas technology to investigate their roles in AF tolerance. Overall, we identified 1865 and 14 novel DEGs in ZM532 and wild-type ZM4. In contrast, 1532 proteins were identified in ZM532 and wild-type ZM4. Among these, we found 96 important genes in ZM532 involving acid resistance mechanisms and survival rates against stressors. Furthermore, our knockout results demonstrated that growth activity and glucose consumption of mutant strains ZM532∆ZMO_RS02740 and ZM4∆ZMO_RS02740 decreased with increased fermentation time from 42 to 55 h and ethanol production up to 58% in ZM532 than that in ZM532∆ZMO_RS02740. Hence, these findings suggest ZMO_RS02740 as a protective strategy for ZM ethanol production under stressful conditions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12934-023-02095-1. BioMed Central 2023-05-02 /pmc/articles/PMC10152622/ /pubmed/37127628 http://dx.doi.org/10.1186/s12934-023-02095-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Shabbir, Samina
Wang, Weiting
Nawaz, Mohsin
Boruah, Prerona
Kulyar, Muhammad Fakhar-e-Alam
Chen, Mao
Wu, Bo
Liu, Panting
Dai, Yonghua
Sun, Lingling
Gou, Qiyu
Liu, Renbin
Hu, Guoquan
Younis, Tahira
He, Mingxiong
Molecular mechanism of engineered Zymomonas mobilis to furfural and acetic acid stress
title Molecular mechanism of engineered Zymomonas mobilis to furfural and acetic acid stress
title_full Molecular mechanism of engineered Zymomonas mobilis to furfural and acetic acid stress
title_fullStr Molecular mechanism of engineered Zymomonas mobilis to furfural and acetic acid stress
title_full_unstemmed Molecular mechanism of engineered Zymomonas mobilis to furfural and acetic acid stress
title_short Molecular mechanism of engineered Zymomonas mobilis to furfural and acetic acid stress
title_sort molecular mechanism of engineered zymomonas mobilis to furfural and acetic acid stress
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10152622/
https://www.ncbi.nlm.nih.gov/pubmed/37127628
http://dx.doi.org/10.1186/s12934-023-02095-1
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