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Molecularly imprinted nanoparticles for pathogen visualisation

Saccharides displayed on the cell surface of pathogens play critical roles in many activities such as adhesion, recognition and pathogenesis, as well as in prokaryotic development. In this work, we report the synthesis of molecularly imprinted nanoparticles (nanoMIPs) against pathogen surface monosa...

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Autores principales: Bezdekova, Jaroslava, Canfarotta, Francesco, Grillo, Fabiana, Yesilkaya, Hasan, Vaculovicova, Marketa, Piletsky, Sergey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10153071/
https://www.ncbi.nlm.nih.gov/pubmed/37143801
http://dx.doi.org/10.1039/d2na00913g
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author Bezdekova, Jaroslava
Canfarotta, Francesco
Grillo, Fabiana
Yesilkaya, Hasan
Vaculovicova, Marketa
Piletsky, Sergey
author_facet Bezdekova, Jaroslava
Canfarotta, Francesco
Grillo, Fabiana
Yesilkaya, Hasan
Vaculovicova, Marketa
Piletsky, Sergey
author_sort Bezdekova, Jaroslava
collection PubMed
description Saccharides displayed on the cell surface of pathogens play critical roles in many activities such as adhesion, recognition and pathogenesis, as well as in prokaryotic development. In this work, we report the synthesis of molecularly imprinted nanoparticles (nanoMIPs) against pathogen surface monosaccharides using an innovative solid-phase approach. These nanoMIPs can serve as robust and selective artificial lectins specific to one particular monosaccharide. The evaluation of their binding capabilities has been implemented against bacterial cells (E. coli and S. pneumoniae) as model pathogens. The nanoMIPs were produced against two different monosaccharides: mannose (Man), which is present mainly on the surface of Gram-negative bacteria, and N-acetylglucosamine (GlcNAc) exposed on the surface of the majority of bacteria. Herein, we assessed the potential use of nanoMIPs for pathogen cell imaging and detection via flow cytometry and confocal microscopy.
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spelling pubmed-101530712023-05-03 Molecularly imprinted nanoparticles for pathogen visualisation Bezdekova, Jaroslava Canfarotta, Francesco Grillo, Fabiana Yesilkaya, Hasan Vaculovicova, Marketa Piletsky, Sergey Nanoscale Adv Chemistry Saccharides displayed on the cell surface of pathogens play critical roles in many activities such as adhesion, recognition and pathogenesis, as well as in prokaryotic development. In this work, we report the synthesis of molecularly imprinted nanoparticles (nanoMIPs) against pathogen surface monosaccharides using an innovative solid-phase approach. These nanoMIPs can serve as robust and selective artificial lectins specific to one particular monosaccharide. The evaluation of their binding capabilities has been implemented against bacterial cells (E. coli and S. pneumoniae) as model pathogens. The nanoMIPs were produced against two different monosaccharides: mannose (Man), which is present mainly on the surface of Gram-negative bacteria, and N-acetylglucosamine (GlcNAc) exposed on the surface of the majority of bacteria. Herein, we assessed the potential use of nanoMIPs for pathogen cell imaging and detection via flow cytometry and confocal microscopy. RSC 2023-03-17 /pmc/articles/PMC10153071/ /pubmed/37143801 http://dx.doi.org/10.1039/d2na00913g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Bezdekova, Jaroslava
Canfarotta, Francesco
Grillo, Fabiana
Yesilkaya, Hasan
Vaculovicova, Marketa
Piletsky, Sergey
Molecularly imprinted nanoparticles for pathogen visualisation
title Molecularly imprinted nanoparticles for pathogen visualisation
title_full Molecularly imprinted nanoparticles for pathogen visualisation
title_fullStr Molecularly imprinted nanoparticles for pathogen visualisation
title_full_unstemmed Molecularly imprinted nanoparticles for pathogen visualisation
title_short Molecularly imprinted nanoparticles for pathogen visualisation
title_sort molecularly imprinted nanoparticles for pathogen visualisation
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10153071/
https://www.ncbi.nlm.nih.gov/pubmed/37143801
http://dx.doi.org/10.1039/d2na00913g
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