Cargando…

“Metformin Impairs Intestinal Fructose Metabolism”

OBJECTIVE: To investigate the effects of metformin on intestinal carbohydrate metabolism in vivo. METHOD: Male mice preconditioned with a high-fat, high-sucrose diet were treated orally with metformin or a control solution for two weeks. Fructose metabolism, glucose production from fructose, and pro...

Descripción completa

Detalles Bibliográficos
Autores principales: Tong, Wenxin, Hannou, Sarah A., Sargsyan, Ashot, Zhang, Guo-Fang, Grimsrud, Paul A., Astapova, Inna, Herman, Mark A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10153158/
https://www.ncbi.nlm.nih.gov/pubmed/37131695
http://dx.doi.org/10.1101/2023.04.17.537251
_version_ 1785035880177598464
author Tong, Wenxin
Hannou, Sarah A.
Sargsyan, Ashot
Zhang, Guo-Fang
Grimsrud, Paul A.
Astapova, Inna
Herman, Mark A.
author_facet Tong, Wenxin
Hannou, Sarah A.
Sargsyan, Ashot
Zhang, Guo-Fang
Grimsrud, Paul A.
Astapova, Inna
Herman, Mark A.
author_sort Tong, Wenxin
collection PubMed
description OBJECTIVE: To investigate the effects of metformin on intestinal carbohydrate metabolism in vivo. METHOD: Male mice preconditioned with a high-fat, high-sucrose diet were treated orally with metformin or a control solution for two weeks. Fructose metabolism, glucose production from fructose, and production of other fructose-derived metabolites were assessed using stably labeled fructose as a tracer. RESULTS: Metformin treatment decreased intestinal glucose levels and reduced incorporation of fructose-derived metabolites into glucose. This was associated with decreased intestinal fructose metabolism as indicated by decreased enterocyte F1P levels and diminished labeling of fructose-derived metabolites. Metformin also reduced fructose delivery to the liver. Proteomic analysis revealed that metformin coordinately down-regulated proteins involved carbohydrate metabolism including those involved in fructolysis and glucose production within intestinal tissue. CONCLUSION: Metformin reduces intestinal fructose metabolism, and this is associated with broad-based changes in intestinal enzyme and protein levels involved in sugar metabolism indicating that metformin's effects on sugar metabolism are pleiotropic.
format Online
Article
Text
id pubmed-10153158
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Cold Spring Harbor Laboratory
record_format MEDLINE/PubMed
spelling pubmed-101531582023-05-03 “Metformin Impairs Intestinal Fructose Metabolism” Tong, Wenxin Hannou, Sarah A. Sargsyan, Ashot Zhang, Guo-Fang Grimsrud, Paul A. Astapova, Inna Herman, Mark A. bioRxiv Article OBJECTIVE: To investigate the effects of metformin on intestinal carbohydrate metabolism in vivo. METHOD: Male mice preconditioned with a high-fat, high-sucrose diet were treated orally with metformin or a control solution for two weeks. Fructose metabolism, glucose production from fructose, and production of other fructose-derived metabolites were assessed using stably labeled fructose as a tracer. RESULTS: Metformin treatment decreased intestinal glucose levels and reduced incorporation of fructose-derived metabolites into glucose. This was associated with decreased intestinal fructose metabolism as indicated by decreased enterocyte F1P levels and diminished labeling of fructose-derived metabolites. Metformin also reduced fructose delivery to the liver. Proteomic analysis revealed that metformin coordinately down-regulated proteins involved carbohydrate metabolism including those involved in fructolysis and glucose production within intestinal tissue. CONCLUSION: Metformin reduces intestinal fructose metabolism, and this is associated with broad-based changes in intestinal enzyme and protein levels involved in sugar metabolism indicating that metformin's effects on sugar metabolism are pleiotropic. Cold Spring Harbor Laboratory 2023-05-15 /pmc/articles/PMC10153158/ /pubmed/37131695 http://dx.doi.org/10.1101/2023.04.17.537251 Text en https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Tong, Wenxin
Hannou, Sarah A.
Sargsyan, Ashot
Zhang, Guo-Fang
Grimsrud, Paul A.
Astapova, Inna
Herman, Mark A.
“Metformin Impairs Intestinal Fructose Metabolism”
title “Metformin Impairs Intestinal Fructose Metabolism”
title_full “Metformin Impairs Intestinal Fructose Metabolism”
title_fullStr “Metformin Impairs Intestinal Fructose Metabolism”
title_full_unstemmed “Metformin Impairs Intestinal Fructose Metabolism”
title_short “Metformin Impairs Intestinal Fructose Metabolism”
title_sort “metformin impairs intestinal fructose metabolism”
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10153158/
https://www.ncbi.nlm.nih.gov/pubmed/37131695
http://dx.doi.org/10.1101/2023.04.17.537251
work_keys_str_mv AT tongwenxin metforminimpairsintestinalfructosemetabolism
AT hannousaraha metforminimpairsintestinalfructosemetabolism
AT sargsyanashot metforminimpairsintestinalfructosemetabolism
AT zhangguofang metforminimpairsintestinalfructosemetabolism
AT grimsrudpaula metforminimpairsintestinalfructosemetabolism
AT astapovainna metforminimpairsintestinalfructosemetabolism
AT hermanmarka metforminimpairsintestinalfructosemetabolism