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Towards engineering a hybrid carboxysome
Carboxysomes are bacterial microcompartments, whose structural features enable the encapsulated Rubisco holoenzyme to operate in a high-CO(2) environment. Consequently, Rubiscos housed within these compartments possess higher catalytic turnover rates relative to their plant counterparts. This partic...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154267/ https://www.ncbi.nlm.nih.gov/pubmed/36892800 http://dx.doi.org/10.1007/s11120-023-01009-x |
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author | Nguyen, Nghiem Dinh Pulsford, Sacha B. Hee, Wei Yi Rae, Benjamin D. Rourke, Loraine M. Price, G. Dean Long, Benedict M. |
author_facet | Nguyen, Nghiem Dinh Pulsford, Sacha B. Hee, Wei Yi Rae, Benjamin D. Rourke, Loraine M. Price, G. Dean Long, Benedict M. |
author_sort | Nguyen, Nghiem Dinh |
collection | PubMed |
description | Carboxysomes are bacterial microcompartments, whose structural features enable the encapsulated Rubisco holoenzyme to operate in a high-CO(2) environment. Consequently, Rubiscos housed within these compartments possess higher catalytic turnover rates relative to their plant counterparts. This particular enzymatic property has made the carboxysome, along with associated transporters, an attractive prospect to incorporate into plant chloroplasts to increase future crop yields. To date, two carboxysome types have been characterized, the α-type that has fewer shell components and the β-type that houses a faster Rubisco. While research is underway to construct a native carboxysome in planta, work investigating the internal arrangement of carboxysomes has identified conserved Rubisco amino acid residues between the two carboxysome types which could be engineered to produce a new, hybrid carboxysome. In theory, this hybrid carboxysome would benefit from the simpler α-carboxysome shell architecture while simultaneously exploiting the higher Rubisco turnover rates in β-carboxysomes. Here, we demonstrate in an Escherichia coli expression system, that the Thermosynechococcus elongatus Form IB Rubisco can be imperfectly incorporated into simplified Cyanobium α-carboxysome-like structures. While encapsulation of non-native cargo can be achieved, T. elongatus Form IB Rubisco does not interact with the Cyanobium carbonic anhydrase, a core requirement for proper carboxysome functionality. Together, these results suggest a way forward to hybrid carboxysome formation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11120-023-01009-x. |
format | Online Article Text |
id | pubmed-10154267 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-101542672023-05-04 Towards engineering a hybrid carboxysome Nguyen, Nghiem Dinh Pulsford, Sacha B. Hee, Wei Yi Rae, Benjamin D. Rourke, Loraine M. Price, G. Dean Long, Benedict M. Photosynth Res Research Carboxysomes are bacterial microcompartments, whose structural features enable the encapsulated Rubisco holoenzyme to operate in a high-CO(2) environment. Consequently, Rubiscos housed within these compartments possess higher catalytic turnover rates relative to their plant counterparts. This particular enzymatic property has made the carboxysome, along with associated transporters, an attractive prospect to incorporate into plant chloroplasts to increase future crop yields. To date, two carboxysome types have been characterized, the α-type that has fewer shell components and the β-type that houses a faster Rubisco. While research is underway to construct a native carboxysome in planta, work investigating the internal arrangement of carboxysomes has identified conserved Rubisco amino acid residues between the two carboxysome types which could be engineered to produce a new, hybrid carboxysome. In theory, this hybrid carboxysome would benefit from the simpler α-carboxysome shell architecture while simultaneously exploiting the higher Rubisco turnover rates in β-carboxysomes. Here, we demonstrate in an Escherichia coli expression system, that the Thermosynechococcus elongatus Form IB Rubisco can be imperfectly incorporated into simplified Cyanobium α-carboxysome-like structures. While encapsulation of non-native cargo can be achieved, T. elongatus Form IB Rubisco does not interact with the Cyanobium carbonic anhydrase, a core requirement for proper carboxysome functionality. Together, these results suggest a way forward to hybrid carboxysome formation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11120-023-01009-x. Springer Netherlands 2023-03-09 2023 /pmc/articles/PMC10154267/ /pubmed/36892800 http://dx.doi.org/10.1007/s11120-023-01009-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Nguyen, Nghiem Dinh Pulsford, Sacha B. Hee, Wei Yi Rae, Benjamin D. Rourke, Loraine M. Price, G. Dean Long, Benedict M. Towards engineering a hybrid carboxysome |
title | Towards engineering a hybrid carboxysome |
title_full | Towards engineering a hybrid carboxysome |
title_fullStr | Towards engineering a hybrid carboxysome |
title_full_unstemmed | Towards engineering a hybrid carboxysome |
title_short | Towards engineering a hybrid carboxysome |
title_sort | towards engineering a hybrid carboxysome |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154267/ https://www.ncbi.nlm.nih.gov/pubmed/36892800 http://dx.doi.org/10.1007/s11120-023-01009-x |
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