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Physaria fendleri FAD3-1 overexpression increases ɑ-linolenic acid content in Camelina sativa seeds

Camelina (Camelina sativa) is an oil crop with a short growing period, resistance to drought and cold, low fertilizer requirements, and can be transformed using floral dipping. Seeds have a high content of polyunsaturated fatty acids, especially ɑ-linolenic acid (ALA), at 32–38%. ALA is an omega-3 f...

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Autores principales: Park, Mid-Eum, Choi, Hyun-A, Kim, Hyun Uk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154323/
https://www.ncbi.nlm.nih.gov/pubmed/37130939
http://dx.doi.org/10.1038/s41598-023-34364-9
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author Park, Mid-Eum
Choi, Hyun-A
Kim, Hyun Uk
author_facet Park, Mid-Eum
Choi, Hyun-A
Kim, Hyun Uk
author_sort Park, Mid-Eum
collection PubMed
description Camelina (Camelina sativa) is an oil crop with a short growing period, resistance to drought and cold, low fertilizer requirements, and can be transformed using floral dipping. Seeds have a high content of polyunsaturated fatty acids, especially ɑ-linolenic acid (ALA), at 32–38%. ALA is an omega-3 fatty acid that is a substrate for eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the human body. In this study, ALA content was further enhanced by the seed-specific expression of Physaria fendleri FAD3-1 (PfFAD3-1) in camelina. The ALA content increased up to 48% in T2 seeds and 50% in T3 seeds. Additionally, size of the seeds increased. The expression of fatty acid metabolism-related genes in PfFAD3-1 OE transgenic lines was different from that in the wild type, where the expression of CsFAD2 decreased and CsFAD3 increased. In summary, we developed a high omega-3 fatty acid-containing camelina with up to 50% ALA content by introducing PfFAD3-1. This line can be used for genetic engineering to obtain EPA and DHA from seeds.
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spelling pubmed-101543232023-05-04 Physaria fendleri FAD3-1 overexpression increases ɑ-linolenic acid content in Camelina sativa seeds Park, Mid-Eum Choi, Hyun-A Kim, Hyun Uk Sci Rep Article Camelina (Camelina sativa) is an oil crop with a short growing period, resistance to drought and cold, low fertilizer requirements, and can be transformed using floral dipping. Seeds have a high content of polyunsaturated fatty acids, especially ɑ-linolenic acid (ALA), at 32–38%. ALA is an omega-3 fatty acid that is a substrate for eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the human body. In this study, ALA content was further enhanced by the seed-specific expression of Physaria fendleri FAD3-1 (PfFAD3-1) in camelina. The ALA content increased up to 48% in T2 seeds and 50% in T3 seeds. Additionally, size of the seeds increased. The expression of fatty acid metabolism-related genes in PfFAD3-1 OE transgenic lines was different from that in the wild type, where the expression of CsFAD2 decreased and CsFAD3 increased. In summary, we developed a high omega-3 fatty acid-containing camelina with up to 50% ALA content by introducing PfFAD3-1. This line can be used for genetic engineering to obtain EPA and DHA from seeds. Nature Publishing Group UK 2023-05-02 /pmc/articles/PMC10154323/ /pubmed/37130939 http://dx.doi.org/10.1038/s41598-023-34364-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Park, Mid-Eum
Choi, Hyun-A
Kim, Hyun Uk
Physaria fendleri FAD3-1 overexpression increases ɑ-linolenic acid content in Camelina sativa seeds
title Physaria fendleri FAD3-1 overexpression increases ɑ-linolenic acid content in Camelina sativa seeds
title_full Physaria fendleri FAD3-1 overexpression increases ɑ-linolenic acid content in Camelina sativa seeds
title_fullStr Physaria fendleri FAD3-1 overexpression increases ɑ-linolenic acid content in Camelina sativa seeds
title_full_unstemmed Physaria fendleri FAD3-1 overexpression increases ɑ-linolenic acid content in Camelina sativa seeds
title_short Physaria fendleri FAD3-1 overexpression increases ɑ-linolenic acid content in Camelina sativa seeds
title_sort physaria fendleri fad3-1 overexpression increases ɑ-linolenic acid content in camelina sativa seeds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154323/
https://www.ncbi.nlm.nih.gov/pubmed/37130939
http://dx.doi.org/10.1038/s41598-023-34364-9
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