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Macrophages from naked mole-rat possess distinct immunometabolic signatures upon polarization

The naked mole-rat (NMR) is a unique long-lived rodent which is highly resistant to age-associated disorders and cancer. The immune system of NMR possesses a distinct cellular composition with the prevalence of myeloid cells. Thus, the detailed phenotypical and functional assessment of NMR myeloid c...

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Autores principales: Gorshkova, Ekaterina A., Gubernatorova, Ekaterina O., Dvorianinova, Ekaterina M., Yurakova, Taisiya R., Marey, Maria V., Averina, Olga A., Holtze, Susanne, Hildebrandt, Thomas B., Dmitriev, Alexey A., Drutskaya, Marina S., Vyssokikh, Mikhail Yu., Nedospasov, Sergei A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154529/
https://www.ncbi.nlm.nih.gov/pubmed/37153552
http://dx.doi.org/10.3389/fimmu.2023.1172467
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author Gorshkova, Ekaterina A.
Gubernatorova, Ekaterina O.
Dvorianinova, Ekaterina M.
Yurakova, Taisiya R.
Marey, Maria V.
Averina, Olga A.
Holtze, Susanne
Hildebrandt, Thomas B.
Dmitriev, Alexey A.
Drutskaya, Marina S.
Vyssokikh, Mikhail Yu.
Nedospasov, Sergei A.
author_facet Gorshkova, Ekaterina A.
Gubernatorova, Ekaterina O.
Dvorianinova, Ekaterina M.
Yurakova, Taisiya R.
Marey, Maria V.
Averina, Olga A.
Holtze, Susanne
Hildebrandt, Thomas B.
Dmitriev, Alexey A.
Drutskaya, Marina S.
Vyssokikh, Mikhail Yu.
Nedospasov, Sergei A.
author_sort Gorshkova, Ekaterina A.
collection PubMed
description The naked mole-rat (NMR) is a unique long-lived rodent which is highly resistant to age-associated disorders and cancer. The immune system of NMR possesses a distinct cellular composition with the prevalence of myeloid cells. Thus, the detailed phenotypical and functional assessment of NMR myeloid cell compartment may uncover novel mechanisms of immunoregulation and healthy aging. In this study gene expression signatures, reactive nitrogen species and cytokine production, as well as metabolic activity of classically (M1) and alternatively (M2) activated NMR bone marrow-derived macrophages (BMDM) were examined. Polarization of NMR macrophages under pro-inflammatory conditions led to expected M1 phenotype characterized by increased pro-inflammatory gene expression, cytokine production and aerobic glycolysis, but paralleled by reduced production of nitric oxide (NO). Under systemic LPS-induced inflammatory conditions NO production also was not detected in NMR blood monocytes. Altogether, our results indicate that NMR macrophages are capable of transcriptional and metabolic reprogramming under polarizing stimuli, however, NMR M1 possesses species-specific signatures as compared to murine M1, implicating distinct adaptations in NMR immune system.
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spelling pubmed-101545292023-05-04 Macrophages from naked mole-rat possess distinct immunometabolic signatures upon polarization Gorshkova, Ekaterina A. Gubernatorova, Ekaterina O. Dvorianinova, Ekaterina M. Yurakova, Taisiya R. Marey, Maria V. Averina, Olga A. Holtze, Susanne Hildebrandt, Thomas B. Dmitriev, Alexey A. Drutskaya, Marina S. Vyssokikh, Mikhail Yu. Nedospasov, Sergei A. Front Immunol Immunology The naked mole-rat (NMR) is a unique long-lived rodent which is highly resistant to age-associated disorders and cancer. The immune system of NMR possesses a distinct cellular composition with the prevalence of myeloid cells. Thus, the detailed phenotypical and functional assessment of NMR myeloid cell compartment may uncover novel mechanisms of immunoregulation and healthy aging. In this study gene expression signatures, reactive nitrogen species and cytokine production, as well as metabolic activity of classically (M1) and alternatively (M2) activated NMR bone marrow-derived macrophages (BMDM) were examined. Polarization of NMR macrophages under pro-inflammatory conditions led to expected M1 phenotype characterized by increased pro-inflammatory gene expression, cytokine production and aerobic glycolysis, but paralleled by reduced production of nitric oxide (NO). Under systemic LPS-induced inflammatory conditions NO production also was not detected in NMR blood monocytes. Altogether, our results indicate that NMR macrophages are capable of transcriptional and metabolic reprogramming under polarizing stimuli, however, NMR M1 possesses species-specific signatures as compared to murine M1, implicating distinct adaptations in NMR immune system. Frontiers Media S.A. 2023-04-19 /pmc/articles/PMC10154529/ /pubmed/37153552 http://dx.doi.org/10.3389/fimmu.2023.1172467 Text en Copyright © 2023 Gorshkova, Gubernatorova, Dvorianinova, Yurakova, Marey, Averina, Holtze, Hildebrandt, Dmitriev, Drutskaya, Vyssokikh and Nedospasov https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Gorshkova, Ekaterina A.
Gubernatorova, Ekaterina O.
Dvorianinova, Ekaterina M.
Yurakova, Taisiya R.
Marey, Maria V.
Averina, Olga A.
Holtze, Susanne
Hildebrandt, Thomas B.
Dmitriev, Alexey A.
Drutskaya, Marina S.
Vyssokikh, Mikhail Yu.
Nedospasov, Sergei A.
Macrophages from naked mole-rat possess distinct immunometabolic signatures upon polarization
title Macrophages from naked mole-rat possess distinct immunometabolic signatures upon polarization
title_full Macrophages from naked mole-rat possess distinct immunometabolic signatures upon polarization
title_fullStr Macrophages from naked mole-rat possess distinct immunometabolic signatures upon polarization
title_full_unstemmed Macrophages from naked mole-rat possess distinct immunometabolic signatures upon polarization
title_short Macrophages from naked mole-rat possess distinct immunometabolic signatures upon polarization
title_sort macrophages from naked mole-rat possess distinct immunometabolic signatures upon polarization
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154529/
https://www.ncbi.nlm.nih.gov/pubmed/37153552
http://dx.doi.org/10.3389/fimmu.2023.1172467
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