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Droplets microfluidics platform—A tool for single cell research

Cells are the most basic structural and functional units of living organisms. Studies of cell growth, differentiation, apoptosis, and cell-cell interactions can help scientists understand the mysteries of living systems. However, there is considerable heterogeneity among cells. Great differences bet...

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Autores principales: Li, Bixuan, Ma, Xi, Cheng, Jianghong, Tian, Tian, Guo, Jiao, Wang, Yang, Pang, Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154550/
https://www.ncbi.nlm.nih.gov/pubmed/37152651
http://dx.doi.org/10.3389/fbioe.2023.1121870
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author Li, Bixuan
Ma, Xi
Cheng, Jianghong
Tian, Tian
Guo, Jiao
Wang, Yang
Pang, Long
author_facet Li, Bixuan
Ma, Xi
Cheng, Jianghong
Tian, Tian
Guo, Jiao
Wang, Yang
Pang, Long
author_sort Li, Bixuan
collection PubMed
description Cells are the most basic structural and functional units of living organisms. Studies of cell growth, differentiation, apoptosis, and cell-cell interactions can help scientists understand the mysteries of living systems. However, there is considerable heterogeneity among cells. Great differences between individuals can be found even within the same cell cluster. Cell heterogeneity can only be clearly expressed and distinguished at the level of single cells. The development of droplet microfluidics technology opens up a new chapter for single-cell analysis. Microfluidic chips can produce many nanoscale monodisperse droplets, which can be used as small isolated micro-laboratories for various high-throughput, precise single-cell analyses. Moreover, gel droplets with good biocompatibility can be used in single-cell cultures and coupled with biomolecules for various downstream analyses of cellular metabolites. The droplets are also maneuverable; through physical and chemical forces, droplets can be divided, fused, and sorted to realize single-cell screening and other related studies. This review describes the channel design, droplet generation, and control technology of droplet microfluidics and gives a detailed overview of the application of droplet microfluidics in single-cell culture, single-cell screening, single-cell detection, and other aspects. Moreover, we provide a recent review of the application of droplet microfluidics in tumor single-cell immunoassays, describe in detail the advantages of microfluidics in tumor research, and predict the development of droplet microfluidics at the single-cell level.
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spelling pubmed-101545502023-05-04 Droplets microfluidics platform—A tool for single cell research Li, Bixuan Ma, Xi Cheng, Jianghong Tian, Tian Guo, Jiao Wang, Yang Pang, Long Front Bioeng Biotechnol Bioengineering and Biotechnology Cells are the most basic structural and functional units of living organisms. Studies of cell growth, differentiation, apoptosis, and cell-cell interactions can help scientists understand the mysteries of living systems. However, there is considerable heterogeneity among cells. Great differences between individuals can be found even within the same cell cluster. Cell heterogeneity can only be clearly expressed and distinguished at the level of single cells. The development of droplet microfluidics technology opens up a new chapter for single-cell analysis. Microfluidic chips can produce many nanoscale monodisperse droplets, which can be used as small isolated micro-laboratories for various high-throughput, precise single-cell analyses. Moreover, gel droplets with good biocompatibility can be used in single-cell cultures and coupled with biomolecules for various downstream analyses of cellular metabolites. The droplets are also maneuverable; through physical and chemical forces, droplets can be divided, fused, and sorted to realize single-cell screening and other related studies. This review describes the channel design, droplet generation, and control technology of droplet microfluidics and gives a detailed overview of the application of droplet microfluidics in single-cell culture, single-cell screening, single-cell detection, and other aspects. Moreover, we provide a recent review of the application of droplet microfluidics in tumor single-cell immunoassays, describe in detail the advantages of microfluidics in tumor research, and predict the development of droplet microfluidics at the single-cell level. Frontiers Media S.A. 2023-04-19 /pmc/articles/PMC10154550/ /pubmed/37152651 http://dx.doi.org/10.3389/fbioe.2023.1121870 Text en Copyright © 2023 Li, Ma, Cheng, Tian, Guo, Wang and Pang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Li, Bixuan
Ma, Xi
Cheng, Jianghong
Tian, Tian
Guo, Jiao
Wang, Yang
Pang, Long
Droplets microfluidics platform—A tool for single cell research
title Droplets microfluidics platform—A tool for single cell research
title_full Droplets microfluidics platform—A tool for single cell research
title_fullStr Droplets microfluidics platform—A tool for single cell research
title_full_unstemmed Droplets microfluidics platform—A tool for single cell research
title_short Droplets microfluidics platform—A tool for single cell research
title_sort droplets microfluidics platform—a tool for single cell research
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154550/
https://www.ncbi.nlm.nih.gov/pubmed/37152651
http://dx.doi.org/10.3389/fbioe.2023.1121870
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