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Exogenous leucine alleviates heat stress and improves saponin synthesis in Panax notoginseng by improving antioxidant capacity and maintaining metabolic homeostasis

Panax notoginseng saponins (PNSs) are used as industrial raw materials to produce many drugs to treat cardio-cerebrovascular diseases. However, it is a heat-sensitive plant, and its large-scale artificial cultivation is impeded by high temperature stress, leading to decreases in productivity and PNS...

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Autores principales: Liu, Haijiao, Su, Yingwei, Fan, Yunxia, Zuo, Denghong, Xu, Jie, Liu, Yixiang, Mei, Xinyue, Huang, Huichuan, Yang, Min, Zhu, Shusheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154563/
https://www.ncbi.nlm.nih.gov/pubmed/37152124
http://dx.doi.org/10.3389/fpls.2023.1175878
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author Liu, Haijiao
Su, Yingwei
Fan, Yunxia
Zuo, Denghong
Xu, Jie
Liu, Yixiang
Mei, Xinyue
Huang, Huichuan
Yang, Min
Zhu, Shusheng
author_facet Liu, Haijiao
Su, Yingwei
Fan, Yunxia
Zuo, Denghong
Xu, Jie
Liu, Yixiang
Mei, Xinyue
Huang, Huichuan
Yang, Min
Zhu, Shusheng
author_sort Liu, Haijiao
collection PubMed
description Panax notoginseng saponins (PNSs) are used as industrial raw materials to produce many drugs to treat cardio-cerebrovascular diseases. However, it is a heat-sensitive plant, and its large-scale artificial cultivation is impeded by high temperature stress, leading to decreases in productivity and PNSs yield. Here, we examined exogenous foliar leucine to alleviate heat stress and explored the underlying mechanism using metabolomics. The results indicated that 3 and 5 mM exogenous foliar leucine significantly alleviated heat stress in one-year- and two-year-old P. notoginseng in pots and field trials. Exogenous foliar leucine enhanced the antioxidant capacity by increasing the activities of antioxidant enzymes (POD, SOD) and the contents of antioxidant metabolites (amino acids). Moreover, exogenous foliar leucine enhanced carbohydrate metabolism, including sugars (sucrose, maltose) and TCA cycle metabolites (citric acid, aconitic acid, succinic acid and fumaric acid), in P. notoginseng leaves, stems, and fibrous roots to improve the energy supply of plants and further alleviate heat stress. Field experiments further verified that exogenous foliar leucine increased the productivity and PNSs accumulation in P. notoginseng. These results suggest that leucine application is beneficial for improving the growth and quality of P. notoginseng under heat stress. It is therefore possible to develop plant growth regulators based on leucine to improve the heat resistance of P. notoginseng and other crops.
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spelling pubmed-101545632023-05-04 Exogenous leucine alleviates heat stress and improves saponin synthesis in Panax notoginseng by improving antioxidant capacity and maintaining metabolic homeostasis Liu, Haijiao Su, Yingwei Fan, Yunxia Zuo, Denghong Xu, Jie Liu, Yixiang Mei, Xinyue Huang, Huichuan Yang, Min Zhu, Shusheng Front Plant Sci Plant Science Panax notoginseng saponins (PNSs) are used as industrial raw materials to produce many drugs to treat cardio-cerebrovascular diseases. However, it is a heat-sensitive plant, and its large-scale artificial cultivation is impeded by high temperature stress, leading to decreases in productivity and PNSs yield. Here, we examined exogenous foliar leucine to alleviate heat stress and explored the underlying mechanism using metabolomics. The results indicated that 3 and 5 mM exogenous foliar leucine significantly alleviated heat stress in one-year- and two-year-old P. notoginseng in pots and field trials. Exogenous foliar leucine enhanced the antioxidant capacity by increasing the activities of antioxidant enzymes (POD, SOD) and the contents of antioxidant metabolites (amino acids). Moreover, exogenous foliar leucine enhanced carbohydrate metabolism, including sugars (sucrose, maltose) and TCA cycle metabolites (citric acid, aconitic acid, succinic acid and fumaric acid), in P. notoginseng leaves, stems, and fibrous roots to improve the energy supply of plants and further alleviate heat stress. Field experiments further verified that exogenous foliar leucine increased the productivity and PNSs accumulation in P. notoginseng. These results suggest that leucine application is beneficial for improving the growth and quality of P. notoginseng under heat stress. It is therefore possible to develop plant growth regulators based on leucine to improve the heat resistance of P. notoginseng and other crops. Frontiers Media S.A. 2023-04-19 /pmc/articles/PMC10154563/ /pubmed/37152124 http://dx.doi.org/10.3389/fpls.2023.1175878 Text en Copyright © 2023 Liu, Su, Fan, Zuo, Xu, Liu, Mei, Huang, Yang and Zhu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Liu, Haijiao
Su, Yingwei
Fan, Yunxia
Zuo, Denghong
Xu, Jie
Liu, Yixiang
Mei, Xinyue
Huang, Huichuan
Yang, Min
Zhu, Shusheng
Exogenous leucine alleviates heat stress and improves saponin synthesis in Panax notoginseng by improving antioxidant capacity and maintaining metabolic homeostasis
title Exogenous leucine alleviates heat stress and improves saponin synthesis in Panax notoginseng by improving antioxidant capacity and maintaining metabolic homeostasis
title_full Exogenous leucine alleviates heat stress and improves saponin synthesis in Panax notoginseng by improving antioxidant capacity and maintaining metabolic homeostasis
title_fullStr Exogenous leucine alleviates heat stress and improves saponin synthesis in Panax notoginseng by improving antioxidant capacity and maintaining metabolic homeostasis
title_full_unstemmed Exogenous leucine alleviates heat stress and improves saponin synthesis in Panax notoginseng by improving antioxidant capacity and maintaining metabolic homeostasis
title_short Exogenous leucine alleviates heat stress and improves saponin synthesis in Panax notoginseng by improving antioxidant capacity and maintaining metabolic homeostasis
title_sort exogenous leucine alleviates heat stress and improves saponin synthesis in panax notoginseng by improving antioxidant capacity and maintaining metabolic homeostasis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154563/
https://www.ncbi.nlm.nih.gov/pubmed/37152124
http://dx.doi.org/10.3389/fpls.2023.1175878
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