Protocol to develop a synthetic biology toolkit for the non-model bacterium R. palustris

Numerous biology tools are developed to work for model organisms, which, however, do not work effectively in non-model organisms. Here, we present a protocol for developing a synthetic biology toolkit for Rhodopseudomonas palustris CGA009, a non-model bacterium with unique metabolic properties. We d...

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Detalles Bibliográficos
Autores principales: Kathol, Mark, Immethun, Cheryl, Saha, Rajib
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154974/
https://www.ncbi.nlm.nih.gov/pubmed/37104094
http://dx.doi.org/10.1016/j.xpro.2023.102158
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author Kathol, Mark
Immethun, Cheryl
Saha, Rajib
author_facet Kathol, Mark
Immethun, Cheryl
Saha, Rajib
author_sort Kathol, Mark
collection PubMed
description Numerous biology tools are developed to work for model organisms, which, however, do not work effectively in non-model organisms. Here, we present a protocol for developing a synthetic biology toolkit for Rhodopseudomonas palustris CGA009, a non-model bacterium with unique metabolic properties. We describe steps for introducing and characterizing biological devices in non-model bacteria, such as the utilization of fluorescence markers and RT-qPCR. This protocol may also be applicable for other non-model organisms. For complete details on the use and execution of this protocol, please refer to Immethun et al..(1)
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spelling pubmed-101549742023-05-04 Protocol to develop a synthetic biology toolkit for the non-model bacterium R. palustris Kathol, Mark Immethun, Cheryl Saha, Rajib STAR Protoc Protocol Numerous biology tools are developed to work for model organisms, which, however, do not work effectively in non-model organisms. Here, we present a protocol for developing a synthetic biology toolkit for Rhodopseudomonas palustris CGA009, a non-model bacterium with unique metabolic properties. We describe steps for introducing and characterizing biological devices in non-model bacteria, such as the utilization of fluorescence markers and RT-qPCR. This protocol may also be applicable for other non-model organisms. For complete details on the use and execution of this protocol, please refer to Immethun et al..(1) Elsevier 2023-04-26 /pmc/articles/PMC10154974/ /pubmed/37104094 http://dx.doi.org/10.1016/j.xpro.2023.102158 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kathol, Mark
Immethun, Cheryl
Saha, Rajib
Protocol to develop a synthetic biology toolkit for the non-model bacterium R. palustris
title Protocol to develop a synthetic biology toolkit for the non-model bacterium R. palustris
title_full Protocol to develop a synthetic biology toolkit for the non-model bacterium R. palustris
title_fullStr Protocol to develop a synthetic biology toolkit for the non-model bacterium R. palustris
title_full_unstemmed Protocol to develop a synthetic biology toolkit for the non-model bacterium R. palustris
title_short Protocol to develop a synthetic biology toolkit for the non-model bacterium R. palustris
title_sort protocol to develop a synthetic biology toolkit for the non-model bacterium r. palustris
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154974/
https://www.ncbi.nlm.nih.gov/pubmed/37104094
http://dx.doi.org/10.1016/j.xpro.2023.102158
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