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High-throughput UV-photofragmentation studies of thymine and guanine

High-throughput photofragmentation studies of thymine and guanine were performed at 257 and 343 nm and for a wide range of ionisation laser intensities. Combining a continuous laser-based thermal desorption source with femtosecond multiphoton ionisation using a 50 kHz repetition rate laser allowed u...

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Autores principales: Wang, Siwen, Dauletyarov, Yerbolat, Krüger, Peter, Horke, Daniel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10155487/
https://www.ncbi.nlm.nih.gov/pubmed/37083208
http://dx.doi.org/10.1039/d3cp00328k
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author Wang, Siwen
Dauletyarov, Yerbolat
Krüger, Peter
Horke, Daniel A.
author_facet Wang, Siwen
Dauletyarov, Yerbolat
Krüger, Peter
Horke, Daniel A.
author_sort Wang, Siwen
collection PubMed
description High-throughput photofragmentation studies of thymine and guanine were performed at 257 and 343 nm and for a wide range of ionisation laser intensities. Combining a continuous laser-based thermal desorption source with femtosecond multiphoton ionisation using a 50 kHz repetition rate laser allowed us to produce detailed 2D maps of fragmentation as a function of incident laser intensity. The fragmentation was distinctly soft, the parent ions being at least an order of magnitude more abundant than fragment ions. For thymine there was a single dominant fragmentation channel, which involves consecutive HNCO and CO losses. In contrast, for guanine there were several competing ones, the most probable channel corresponding to CH(2)N(2) loss through opening of the pyrimidine ring. The dependence of parent ion abundance on the ionisation laser intensity showed that at 257 nm the ionisation of thymine is a 1 + 1 resonance enhanced process through its open-shell singlet state.
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spelling pubmed-101554872023-05-04 High-throughput UV-photofragmentation studies of thymine and guanine Wang, Siwen Dauletyarov, Yerbolat Krüger, Peter Horke, Daniel A. Phys Chem Chem Phys Chemistry High-throughput photofragmentation studies of thymine and guanine were performed at 257 and 343 nm and for a wide range of ionisation laser intensities. Combining a continuous laser-based thermal desorption source with femtosecond multiphoton ionisation using a 50 kHz repetition rate laser allowed us to produce detailed 2D maps of fragmentation as a function of incident laser intensity. The fragmentation was distinctly soft, the parent ions being at least an order of magnitude more abundant than fragment ions. For thymine there was a single dominant fragmentation channel, which involves consecutive HNCO and CO losses. In contrast, for guanine there were several competing ones, the most probable channel corresponding to CH(2)N(2) loss through opening of the pyrimidine ring. The dependence of parent ion abundance on the ionisation laser intensity showed that at 257 nm the ionisation of thymine is a 1 + 1 resonance enhanced process through its open-shell singlet state. The Royal Society of Chemistry 2023-04-17 /pmc/articles/PMC10155487/ /pubmed/37083208 http://dx.doi.org/10.1039/d3cp00328k Text en This journal is © the Owner Societies https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Wang, Siwen
Dauletyarov, Yerbolat
Krüger, Peter
Horke, Daniel A.
High-throughput UV-photofragmentation studies of thymine and guanine
title High-throughput UV-photofragmentation studies of thymine and guanine
title_full High-throughput UV-photofragmentation studies of thymine and guanine
title_fullStr High-throughput UV-photofragmentation studies of thymine and guanine
title_full_unstemmed High-throughput UV-photofragmentation studies of thymine and guanine
title_short High-throughput UV-photofragmentation studies of thymine and guanine
title_sort high-throughput uv-photofragmentation studies of thymine and guanine
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10155487/
https://www.ncbi.nlm.nih.gov/pubmed/37083208
http://dx.doi.org/10.1039/d3cp00328k
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