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Circ_0068631 sponges miR‐139‐5p to promote the growth and metastasis of cutaneous squamous cell carcinoma by upregulating HOXB7
BACKGROUND: Circular RNAs (circRNAs) are often dysregulated in cancers and closely related to cancer progression, including cutaneous squamous cell carcinoma (CSCC). However, the role and mechanism of circ_0068631 in CSCC progression have not been reported. METHODS: The expression of circ_0068631, m...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10155854/ https://www.ncbi.nlm.nih.gov/pubmed/36823512 http://dx.doi.org/10.1111/srt.13248 |
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author | Ji, Jun Xiong, Chengcheng Peng, Jing Zhang, Niannian Zhang, Yan Yang, Honghong Zhu, Wenwen |
author_facet | Ji, Jun Xiong, Chengcheng Peng, Jing Zhang, Niannian Zhang, Yan Yang, Honghong Zhu, Wenwen |
author_sort | Ji, Jun |
collection | PubMed |
description | BACKGROUND: Circular RNAs (circRNAs) are often dysregulated in cancers and closely related to cancer progression, including cutaneous squamous cell carcinoma (CSCC). However, the role and mechanism of circ_0068631 in CSCC progression have not been reported. METHODS: The expression of circ_0068631, microRNA‐139‐5p (miR‐139‐5p), and homeobox B7 (HOXB7) was measured by real‐time quantitative polymerase chain reaction (RT‐qPCR). Cell counting kit‐8 (CCK‐8) assay, 5‐ethynyl‐2′‐deoxyuridine (EdU) assay, and colony formation assay were used to measure cell proliferation. Cell apoptosis was assessed by flow cytometry. Cell migration was detected by transwell assay. The interaction between miR‐139‐5p and circ_0068631 or HOXB7 was confirmed by dual‐luciferase reporter assay. A xenograft tumor model was established to confirm the function of circ_0068631 in vivo. RESULTS: Circ_0068631 was upregulated in CSCC tissues and cells, and its silencing could inhibit CSCC cell proliferation and metastasis while promoting apoptosis in vitro, as well as restrain CSCC tumor growth in vivo. Circ_0068631 acted as a sponge of miR‐139‐5p, and miR‐139‐5p inhibition reversed the repressive effect of circ_0068631 knockdown on CSCC cell progression. Furthermore, HOXB7 was a target of miR‐139‐5p, and miR‐139‐5p inhibited the malignant behaviors by downregulating HOXB7 expression in CSCC cells. Further, circ_0068631 sponged miR‐139‐5p to regulate HOXB7 expression. CONCLUSION: Circ_0068631 functioned as a novel oncogene in CSCC progression by regulating miR‐139‐5p/HOXB7 axis, suggesting that circ_0068631 may be a potential target for CSCC treatment. HIGHLIGHTS: Circ_0068631 was overexpressed in CSCC tissues and cells. Circ_0068631 downregulation suppressed CSCC progression via miR‐139‐5p. Circ_0068631 regulated HOXB7 via sponging miR‐139‐5p. |
format | Online Article Text |
id | pubmed-10155854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101558542023-08-11 Circ_0068631 sponges miR‐139‐5p to promote the growth and metastasis of cutaneous squamous cell carcinoma by upregulating HOXB7 Ji, Jun Xiong, Chengcheng Peng, Jing Zhang, Niannian Zhang, Yan Yang, Honghong Zhu, Wenwen Skin Res Technol Original Articles BACKGROUND: Circular RNAs (circRNAs) are often dysregulated in cancers and closely related to cancer progression, including cutaneous squamous cell carcinoma (CSCC). However, the role and mechanism of circ_0068631 in CSCC progression have not been reported. METHODS: The expression of circ_0068631, microRNA‐139‐5p (miR‐139‐5p), and homeobox B7 (HOXB7) was measured by real‐time quantitative polymerase chain reaction (RT‐qPCR). Cell counting kit‐8 (CCK‐8) assay, 5‐ethynyl‐2′‐deoxyuridine (EdU) assay, and colony formation assay were used to measure cell proliferation. Cell apoptosis was assessed by flow cytometry. Cell migration was detected by transwell assay. The interaction between miR‐139‐5p and circ_0068631 or HOXB7 was confirmed by dual‐luciferase reporter assay. A xenograft tumor model was established to confirm the function of circ_0068631 in vivo. RESULTS: Circ_0068631 was upregulated in CSCC tissues and cells, and its silencing could inhibit CSCC cell proliferation and metastasis while promoting apoptosis in vitro, as well as restrain CSCC tumor growth in vivo. Circ_0068631 acted as a sponge of miR‐139‐5p, and miR‐139‐5p inhibition reversed the repressive effect of circ_0068631 knockdown on CSCC cell progression. Furthermore, HOXB7 was a target of miR‐139‐5p, and miR‐139‐5p inhibited the malignant behaviors by downregulating HOXB7 expression in CSCC cells. Further, circ_0068631 sponged miR‐139‐5p to regulate HOXB7 expression. CONCLUSION: Circ_0068631 functioned as a novel oncogene in CSCC progression by regulating miR‐139‐5p/HOXB7 axis, suggesting that circ_0068631 may be a potential target for CSCC treatment. HIGHLIGHTS: Circ_0068631 was overexpressed in CSCC tissues and cells. Circ_0068631 downregulation suppressed CSCC progression via miR‐139‐5p. Circ_0068631 regulated HOXB7 via sponging miR‐139‐5p. John Wiley and Sons Inc. 2023-02-12 /pmc/articles/PMC10155854/ /pubmed/36823512 http://dx.doi.org/10.1111/srt.13248 Text en © 2022 The Authors. Skin Research and Technology published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Original Articles Ji, Jun Xiong, Chengcheng Peng, Jing Zhang, Niannian Zhang, Yan Yang, Honghong Zhu, Wenwen Circ_0068631 sponges miR‐139‐5p to promote the growth and metastasis of cutaneous squamous cell carcinoma by upregulating HOXB7 |
title | Circ_0068631 sponges miR‐139‐5p to promote the growth and metastasis of cutaneous squamous cell carcinoma by upregulating HOXB7 |
title_full | Circ_0068631 sponges miR‐139‐5p to promote the growth and metastasis of cutaneous squamous cell carcinoma by upregulating HOXB7 |
title_fullStr | Circ_0068631 sponges miR‐139‐5p to promote the growth and metastasis of cutaneous squamous cell carcinoma by upregulating HOXB7 |
title_full_unstemmed | Circ_0068631 sponges miR‐139‐5p to promote the growth and metastasis of cutaneous squamous cell carcinoma by upregulating HOXB7 |
title_short | Circ_0068631 sponges miR‐139‐5p to promote the growth and metastasis of cutaneous squamous cell carcinoma by upregulating HOXB7 |
title_sort | circ_0068631 sponges mir‐139‐5p to promote the growth and metastasis of cutaneous squamous cell carcinoma by upregulating hoxb7 |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10155854/ https://www.ncbi.nlm.nih.gov/pubmed/36823512 http://dx.doi.org/10.1111/srt.13248 |
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