Comparative antibacterial effects of ginger and marjoram extract versus conventional irrigants on mature Enterococcus faecalis biofilms: An in vitro study

BACKGROUND: This study evaluated antibacterial effects of Ginger and Marjoram extract compared with Routine Intracanal Irrigants on Mature Enterococcus faecalis Biofilms. MATERIAL AND METHODS: Sixty-six extracted human teeth, were randomly assigned to four groups 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), chloroform extract of marjoram (Origanum majorana), and oil extract of ginger (Zingiber officinale), and two positive and negative control groups (n=11). Samples were contaminated with E. faecalis, except the negative control group. Then the root canals were irrigated with solutions above, after which dental debris was collected from each tooth separately, followed by culturing on plates containing BHI agar. The bacterial counts were finally determined with a colony counting machine. RESULTS: No bacterial growth was detected in the NaOCl, CHX, and negative control groups. However, some bacterial growth was observed in the ginger and marjoram groups. All four solutions successfully eliminated E. faecalis biofilms compared to the positive control group. Significant difference in the median bacterial growth between the ginger and marjoram groups and the positive control group (P<0.001) has been shown. There was no significant difference in median bacterial growth between the ginger and marjoram groups (P=0.94). CONCLUSIONS: Chloroform extract of marjoram and oil extract of ginger were effective in eliminating 6-week-old biofilms of E. faecalis in vitro; however, their effect was not as favorable as CHX and NaOCl. These herbal extracts yielded promising results in the present study; therefore, they can be used as intracanal irrigants instead of chemical agents. Key words:Biofilm, Chlorhexidine, E. faecalis, Ginger, Marjoram, Sodium hypochlorite.