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Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate
Athletic conditioning can increase the capacity for insulin-stimulated skeletal muscle glucose uptake through increased sarcolemmal expression of GLUT4 and potentially additional novel glucose transporters. We used a canine model that has previously demonstrated conditioning-induced increases in bas...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10155965/ https://www.ncbi.nlm.nih.gov/pubmed/37134107 http://dx.doi.org/10.1371/journal.pone.0285424 |
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author | Barrett, Montana Renae Scott Davis, Michael |
author_facet | Barrett, Montana Renae Scott Davis, Michael |
author_sort | Barrett, Montana Renae |
collection | PubMed |
description | Athletic conditioning can increase the capacity for insulin-stimulated skeletal muscle glucose uptake through increased sarcolemmal expression of GLUT4 and potentially additional novel glucose transporters. We used a canine model that has previously demonstrated conditioning-induced increases in basal, insulin- and contraction-stimulated glucose uptake to identify whether expression of glucose transporters other than GLUT4 was upregulated by athletic conditioning. Skeletal muscle biopsies were obtained from 12 adult Alaskan Husky racing sled dogs before and after a full season of conditioning and racing, and homogenates from those biopsies were assayed for expression of GLUT1, GLUT3, GLUT4, GLUT6, GLUT8, and GLUT12 using western blots. Athletic conditioning resulted in a 1.31 ± 0.70 fold increase in GLUT1 (p <0.0001), 1.80 ± 1.99 fold increase in GLUT4 (p = 0.005), and 2.46 ± 2.39 fold increase in GLUT12 (p = 0.002). The increased expression of GLUT1 helps explain the previous findings of conditioning-induced increases in basal glucose clearance in this model, and the increase in GLUT12 provides an alternative mechanism for insulin- and contraction-mediated glucose uptake and likely contributes to the substantial conditioning-induced increases in insulin sensitivity in highly trained athletic dogs. Furthermore, these results suggest that athletic dogs can serve as a valuable resource for the study of alternative glucose transport mechanisms in higher mammals. |
format | Online Article Text |
id | pubmed-10155965 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-101559652023-05-04 Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate Barrett, Montana Renae Scott Davis, Michael PLoS One Research Article Athletic conditioning can increase the capacity for insulin-stimulated skeletal muscle glucose uptake through increased sarcolemmal expression of GLUT4 and potentially additional novel glucose transporters. We used a canine model that has previously demonstrated conditioning-induced increases in basal, insulin- and contraction-stimulated glucose uptake to identify whether expression of glucose transporters other than GLUT4 was upregulated by athletic conditioning. Skeletal muscle biopsies were obtained from 12 adult Alaskan Husky racing sled dogs before and after a full season of conditioning and racing, and homogenates from those biopsies were assayed for expression of GLUT1, GLUT3, GLUT4, GLUT6, GLUT8, and GLUT12 using western blots. Athletic conditioning resulted in a 1.31 ± 0.70 fold increase in GLUT1 (p <0.0001), 1.80 ± 1.99 fold increase in GLUT4 (p = 0.005), and 2.46 ± 2.39 fold increase in GLUT12 (p = 0.002). The increased expression of GLUT1 helps explain the previous findings of conditioning-induced increases in basal glucose clearance in this model, and the increase in GLUT12 provides an alternative mechanism for insulin- and contraction-mediated glucose uptake and likely contributes to the substantial conditioning-induced increases in insulin sensitivity in highly trained athletic dogs. Furthermore, these results suggest that athletic dogs can serve as a valuable resource for the study of alternative glucose transport mechanisms in higher mammals. Public Library of Science 2023-05-03 /pmc/articles/PMC10155965/ /pubmed/37134107 http://dx.doi.org/10.1371/journal.pone.0285424 Text en © 2023 Barrett, Scott Davis https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Barrett, Montana Renae Scott Davis, Michael Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate |
title | Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate |
title_full | Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate |
title_fullStr | Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate |
title_full_unstemmed | Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate |
title_short | Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate |
title_sort | conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10155965/ https://www.ncbi.nlm.nih.gov/pubmed/37134107 http://dx.doi.org/10.1371/journal.pone.0285424 |
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