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Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate

Athletic conditioning can increase the capacity for insulin-stimulated skeletal muscle glucose uptake through increased sarcolemmal expression of GLUT4 and potentially additional novel glucose transporters. We used a canine model that has previously demonstrated conditioning-induced increases in bas...

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Autores principales: Barrett, Montana Renae, Scott Davis, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10155965/
https://www.ncbi.nlm.nih.gov/pubmed/37134107
http://dx.doi.org/10.1371/journal.pone.0285424
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author Barrett, Montana Renae
Scott Davis, Michael
author_facet Barrett, Montana Renae
Scott Davis, Michael
author_sort Barrett, Montana Renae
collection PubMed
description Athletic conditioning can increase the capacity for insulin-stimulated skeletal muscle glucose uptake through increased sarcolemmal expression of GLUT4 and potentially additional novel glucose transporters. We used a canine model that has previously demonstrated conditioning-induced increases in basal, insulin- and contraction-stimulated glucose uptake to identify whether expression of glucose transporters other than GLUT4 was upregulated by athletic conditioning. Skeletal muscle biopsies were obtained from 12 adult Alaskan Husky racing sled dogs before and after a full season of conditioning and racing, and homogenates from those biopsies were assayed for expression of GLUT1, GLUT3, GLUT4, GLUT6, GLUT8, and GLUT12 using western blots. Athletic conditioning resulted in a 1.31 ± 0.70 fold increase in GLUT1 (p <0.0001), 1.80 ± 1.99 fold increase in GLUT4 (p = 0.005), and 2.46 ± 2.39 fold increase in GLUT12 (p = 0.002). The increased expression of GLUT1 helps explain the previous findings of conditioning-induced increases in basal glucose clearance in this model, and the increase in GLUT12 provides an alternative mechanism for insulin- and contraction-mediated glucose uptake and likely contributes to the substantial conditioning-induced increases in insulin sensitivity in highly trained athletic dogs. Furthermore, these results suggest that athletic dogs can serve as a valuable resource for the study of alternative glucose transport mechanisms in higher mammals.
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spelling pubmed-101559652023-05-04 Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate Barrett, Montana Renae Scott Davis, Michael PLoS One Research Article Athletic conditioning can increase the capacity for insulin-stimulated skeletal muscle glucose uptake through increased sarcolemmal expression of GLUT4 and potentially additional novel glucose transporters. We used a canine model that has previously demonstrated conditioning-induced increases in basal, insulin- and contraction-stimulated glucose uptake to identify whether expression of glucose transporters other than GLUT4 was upregulated by athletic conditioning. Skeletal muscle biopsies were obtained from 12 adult Alaskan Husky racing sled dogs before and after a full season of conditioning and racing, and homogenates from those biopsies were assayed for expression of GLUT1, GLUT3, GLUT4, GLUT6, GLUT8, and GLUT12 using western blots. Athletic conditioning resulted in a 1.31 ± 0.70 fold increase in GLUT1 (p <0.0001), 1.80 ± 1.99 fold increase in GLUT4 (p = 0.005), and 2.46 ± 2.39 fold increase in GLUT12 (p = 0.002). The increased expression of GLUT1 helps explain the previous findings of conditioning-induced increases in basal glucose clearance in this model, and the increase in GLUT12 provides an alternative mechanism for insulin- and contraction-mediated glucose uptake and likely contributes to the substantial conditioning-induced increases in insulin sensitivity in highly trained athletic dogs. Furthermore, these results suggest that athletic dogs can serve as a valuable resource for the study of alternative glucose transport mechanisms in higher mammals. Public Library of Science 2023-05-03 /pmc/articles/PMC10155965/ /pubmed/37134107 http://dx.doi.org/10.1371/journal.pone.0285424 Text en © 2023 Barrett, Scott Davis https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Barrett, Montana Renae
Scott Davis, Michael
Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate
title Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate
title_full Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate
title_fullStr Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate
title_full_unstemmed Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate
title_short Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate
title_sort conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10155965/
https://www.ncbi.nlm.nih.gov/pubmed/37134107
http://dx.doi.org/10.1371/journal.pone.0285424
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