The Synthesis and Transfection Activity of Disulfide Polycationic Amphiphiles

Some new polycationic amphiphiles containing a disulfide group were synthesized. Cationic liposomes formed from the compounds synthesized and a helper lipid 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine were not toxic for HEK293 and HeLa cells and were highly effective when delivering a fluores...

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Detalles Bibliográficos
Autores principales: Petukhov, I. A., Puchkov, P. A., Morozova, N. G., Zenkova, M. A., Maslov, M. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10156423/
https://www.ncbi.nlm.nih.gov/pubmed/37192981
http://dx.doi.org/10.1134/S1068162023010235
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author Petukhov, I. A.
Puchkov, P. A.
Morozova, N. G.
Zenkova, M. A.
Maslov, M. A.
author_facet Petukhov, I. A.
Puchkov, P. A.
Morozova, N. G.
Zenkova, M. A.
Maslov, M. A.
author_sort Petukhov, I. A.
collection PubMed
description Some new polycationic amphiphiles containing a disulfide group were synthesized. Cationic liposomes formed from the compounds synthesized and a helper lipid 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine were not toxic for HEK293 and HeLa cells and were highly effective when delivering a fluorescently labeled oligodeoxyribonucleotide. The efficacy of plasmid DNA delivery depended on the cell line and the amphiphile structure, liposomes based on tetracationic amphiphiles being the most effective transfectants. These liposomes can be used for in vitro transfection of eukaryotic cells as well as for further in vivo biological studies.
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spelling pubmed-101564232023-05-09 The Synthesis and Transfection Activity of Disulfide Polycationic Amphiphiles Petukhov, I. A. Puchkov, P. A. Morozova, N. G. Zenkova, M. A. Maslov, M. A. Russ J Bioorg Chem Article Some new polycationic amphiphiles containing a disulfide group were synthesized. Cationic liposomes formed from the compounds synthesized and a helper lipid 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine were not toxic for HEK293 and HeLa cells and were highly effective when delivering a fluorescently labeled oligodeoxyribonucleotide. The efficacy of plasmid DNA delivery depended on the cell line and the amphiphile structure, liposomes based on tetracationic amphiphiles being the most effective transfectants. These liposomes can be used for in vitro transfection of eukaryotic cells as well as for further in vivo biological studies. Pleiades Publishing 2023-05-03 2023 /pmc/articles/PMC10156423/ /pubmed/37192981 http://dx.doi.org/10.1134/S1068162023010235 Text en © Pleiades Publishing, Ltd. 2023, ISSN 1068-1620, Russian Journal of Bioorganic Chemistry, 2023, Vol. 49, No. 1, pp. 41–51. © Pleiades Publishing, Ltd., 2023.Russian Text © The Author(s), 2023, published in Bioorganicheskaya Khimiya, 2023, Vol. 49, No. 2, pp. 165–177. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Petukhov, I. A.
Puchkov, P. A.
Morozova, N. G.
Zenkova, M. A.
Maslov, M. A.
The Synthesis and Transfection Activity of Disulfide Polycationic Amphiphiles
title The Synthesis and Transfection Activity of Disulfide Polycationic Amphiphiles
title_full The Synthesis and Transfection Activity of Disulfide Polycationic Amphiphiles
title_fullStr The Synthesis and Transfection Activity of Disulfide Polycationic Amphiphiles
title_full_unstemmed The Synthesis and Transfection Activity of Disulfide Polycationic Amphiphiles
title_short The Synthesis and Transfection Activity of Disulfide Polycationic Amphiphiles
title_sort synthesis and transfection activity of disulfide polycationic amphiphiles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10156423/
https://www.ncbi.nlm.nih.gov/pubmed/37192981
http://dx.doi.org/10.1134/S1068162023010235
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