Cargando…

The ratios of dietary non-fibrous carbohydrate (NFC) to neutral detergent fiber (NDF) influence intestinal immunity of rabbits by regulating gut microbiota composition and metabolites

Carbohydrate is the most common macronutrient consumed across all phases of the diet and acts as a potential regulator in modulating the gut microbiota in animals. However, the influences of dietary non-fibrous carbohydrate (NFC) to neutral detergent fiber (NDF) in different ratios on gut microbiota...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Shuo, Liu, Tingting, Wang, Kun, Li, Chong, Wu, Fengyang, Yang, Xinyu, Zhao, Man, Chen, Baojiang, Chen, Xiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10157162/
https://www.ncbi.nlm.nih.gov/pubmed/37152746
http://dx.doi.org/10.3389/fmicb.2023.1146787
_version_ 1785036691257425920
author Li, Shuo
Liu, Tingting
Wang, Kun
Li, Chong
Wu, Fengyang
Yang, Xinyu
Zhao, Man
Chen, Baojiang
Chen, Xiang
author_facet Li, Shuo
Liu, Tingting
Wang, Kun
Li, Chong
Wu, Fengyang
Yang, Xinyu
Zhao, Man
Chen, Baojiang
Chen, Xiang
author_sort Li, Shuo
collection PubMed
description Carbohydrate is the most common macronutrient consumed across all phases of the diet and acts as a potential regulator in modulating the gut microbiota in animals. However, the influences of dietary non-fibrous carbohydrate (NFC) to neutral detergent fiber (NDF) in different ratios on gut microbiota, metabolites, intestinal immunity, and growth performance have not been fully explored. A total of 135 healthy weaned rabbits (45.1 ± 0.7 d of age) with an average body weight of 1.08 ± 0.07 kg were randomly divided into five groups. Under the same other nutrient levels, rabbits were fed diets with NFC/NDF ratios of 0.7 (T1), 1.0 (T2), 1.3 (T3), 1.6 (T4), and 1.9 (T5). During the 28-day experiment, T3 rabbits showed the highest final body weight and the lowest feed-to-weight ratio than T5 rabbits (P < 0.05) but no significant difference with T1 or T2 rabbits. The expression of cecal pro-inflammatory factors IL-1β and TNF-α was increased in the T4 and T5 than in those of other groups (P < 0.05). Conversely, the tight junction proteins (ZO-1, Claudin-1, and Occludin) were decreased to varying degrees in the T4 and T5 groups. The pH value in the cecal digesta of T5 rabbits was lower than that of T1, T2, and T3 (P < 0.05), while the concentration of volatile fatty acids and propionate was higher than those of T1, T2, and T3 rabbits (P < 0.05). In terms of gut microbiota, at the phylum level, the relative burden of Firmicutes and Actinobacteria in T2 rabbits was the highest (P < 0.05), and the relative burden of Proteobacteria in T5 rabbits was higher than that of other groups (P < 0.05). At the genus level, the relative burden of Ruminococcus was higher in T2 and T3 rabbits than that of other groups, and T5 rabbits have the lowest relative burden of Ruminococcus. Combination analysis showed that cecal metabolites were positively associated with fermentation-related phenotypes and the burden of Firmicutes (P < 0.05). In conclusion, different dietary NFC/NDF ratios can affect the intestinal immune response and growth performance of rabbits, and there was a positive effect when dietary NFC/NDF = 1.0–1.3.
format Online
Article
Text
id pubmed-10157162
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-101571622023-05-05 The ratios of dietary non-fibrous carbohydrate (NFC) to neutral detergent fiber (NDF) influence intestinal immunity of rabbits by regulating gut microbiota composition and metabolites Li, Shuo Liu, Tingting Wang, Kun Li, Chong Wu, Fengyang Yang, Xinyu Zhao, Man Chen, Baojiang Chen, Xiang Front Microbiol Microbiology Carbohydrate is the most common macronutrient consumed across all phases of the diet and acts as a potential regulator in modulating the gut microbiota in animals. However, the influences of dietary non-fibrous carbohydrate (NFC) to neutral detergent fiber (NDF) in different ratios on gut microbiota, metabolites, intestinal immunity, and growth performance have not been fully explored. A total of 135 healthy weaned rabbits (45.1 ± 0.7 d of age) with an average body weight of 1.08 ± 0.07 kg were randomly divided into five groups. Under the same other nutrient levels, rabbits were fed diets with NFC/NDF ratios of 0.7 (T1), 1.0 (T2), 1.3 (T3), 1.6 (T4), and 1.9 (T5). During the 28-day experiment, T3 rabbits showed the highest final body weight and the lowest feed-to-weight ratio than T5 rabbits (P < 0.05) but no significant difference with T1 or T2 rabbits. The expression of cecal pro-inflammatory factors IL-1β and TNF-α was increased in the T4 and T5 than in those of other groups (P < 0.05). Conversely, the tight junction proteins (ZO-1, Claudin-1, and Occludin) were decreased to varying degrees in the T4 and T5 groups. The pH value in the cecal digesta of T5 rabbits was lower than that of T1, T2, and T3 (P < 0.05), while the concentration of volatile fatty acids and propionate was higher than those of T1, T2, and T3 rabbits (P < 0.05). In terms of gut microbiota, at the phylum level, the relative burden of Firmicutes and Actinobacteria in T2 rabbits was the highest (P < 0.05), and the relative burden of Proteobacteria in T5 rabbits was higher than that of other groups (P < 0.05). At the genus level, the relative burden of Ruminococcus was higher in T2 and T3 rabbits than that of other groups, and T5 rabbits have the lowest relative burden of Ruminococcus. Combination analysis showed that cecal metabolites were positively associated with fermentation-related phenotypes and the burden of Firmicutes (P < 0.05). In conclusion, different dietary NFC/NDF ratios can affect the intestinal immune response and growth performance of rabbits, and there was a positive effect when dietary NFC/NDF = 1.0–1.3. Frontiers Media S.A. 2023-04-20 /pmc/articles/PMC10157162/ /pubmed/37152746 http://dx.doi.org/10.3389/fmicb.2023.1146787 Text en Copyright © 2023 Li, Liu, Wang, Li, Wu, Yang, Zhao, Chen and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Shuo
Liu, Tingting
Wang, Kun
Li, Chong
Wu, Fengyang
Yang, Xinyu
Zhao, Man
Chen, Baojiang
Chen, Xiang
The ratios of dietary non-fibrous carbohydrate (NFC) to neutral detergent fiber (NDF) influence intestinal immunity of rabbits by regulating gut microbiota composition and metabolites
title The ratios of dietary non-fibrous carbohydrate (NFC) to neutral detergent fiber (NDF) influence intestinal immunity of rabbits by regulating gut microbiota composition and metabolites
title_full The ratios of dietary non-fibrous carbohydrate (NFC) to neutral detergent fiber (NDF) influence intestinal immunity of rabbits by regulating gut microbiota composition and metabolites
title_fullStr The ratios of dietary non-fibrous carbohydrate (NFC) to neutral detergent fiber (NDF) influence intestinal immunity of rabbits by regulating gut microbiota composition and metabolites
title_full_unstemmed The ratios of dietary non-fibrous carbohydrate (NFC) to neutral detergent fiber (NDF) influence intestinal immunity of rabbits by regulating gut microbiota composition and metabolites
title_short The ratios of dietary non-fibrous carbohydrate (NFC) to neutral detergent fiber (NDF) influence intestinal immunity of rabbits by regulating gut microbiota composition and metabolites
title_sort ratios of dietary non-fibrous carbohydrate (nfc) to neutral detergent fiber (ndf) influence intestinal immunity of rabbits by regulating gut microbiota composition and metabolites
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10157162/
https://www.ncbi.nlm.nih.gov/pubmed/37152746
http://dx.doi.org/10.3389/fmicb.2023.1146787
work_keys_str_mv AT lishuo theratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT liutingting theratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT wangkun theratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT lichong theratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT wufengyang theratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT yangxinyu theratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT zhaoman theratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT chenbaojiang theratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT chenxiang theratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT lishuo ratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT liutingting ratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT wangkun ratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT lichong ratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT wufengyang ratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT yangxinyu ratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT zhaoman ratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT chenbaojiang ratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites
AT chenxiang ratiosofdietarynonfibrouscarbohydratenfctoneutraldetergentfiberndfinfluenceintestinalimmunityofrabbitsbyregulatinggutmicrobiotacompositionandmetabolites