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Fine mapping and candidate gene analysis of gynoecy trait in chieh-qua (Benincasa hispida Cogn. var. chieh-qua How)

Gynoecy demonstrates an earlier production of hybrids and a higher yield and improves the efficiency of hybrid seed production. Therefore, the utilization of gynoecy is beneficial for the genetic breeding of chieh-qua. However, little knowledge of gynoecious-related genes in chieh-qua has been repor...

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Autores principales: Wang, Min, Yang, Songguang, Liu, Wei, Cao, Zhenqiang, Chen, Lin, Liu, Wenrui, Xie, Dasen, Yan, Jinqiang, Jiang, Biao, Peng, Qingwu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10157166/
https://www.ncbi.nlm.nih.gov/pubmed/37152167
http://dx.doi.org/10.3389/fpls.2023.1158735
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author Wang, Min
Yang, Songguang
Liu, Wei
Cao, Zhenqiang
Chen, Lin
Liu, Wenrui
Xie, Dasen
Yan, Jinqiang
Jiang, Biao
Peng, Qingwu
author_facet Wang, Min
Yang, Songguang
Liu, Wei
Cao, Zhenqiang
Chen, Lin
Liu, Wenrui
Xie, Dasen
Yan, Jinqiang
Jiang, Biao
Peng, Qingwu
author_sort Wang, Min
collection PubMed
description Gynoecy demonstrates an earlier production of hybrids and a higher yield and improves the efficiency of hybrid seed production. Therefore, the utilization of gynoecy is beneficial for the genetic breeding of chieh-qua. However, little knowledge of gynoecious-related genes in chieh-qua has been reported until now. Here, we used an F(2) population from the cross between the gynoecious line ‘A36’ and the monoecious line ‘SX’ for genetic mapping and revealed that chieh-qua gynoecy was regulated by a single recessive gene. We fine-mapped it into a 530-kb region flanked by the markers Indel-3 and KASP145 on Chr.8, which harbors eight candidate genes. One of the candidate genes, Bhi08G000345, encoding networked protein 4 (CqNET4), contained a non-synonymous SNP resulting in the amino acid substitution of isoleucine (ATA; I) to methionine (ATG; M). CqNET4 was prominently expressed in the female flower, and only three genes related to ethylene synthesis were significantly expressed between ‘A36’ and ‘SX.’ The results presented here provide support for the CqNET4 as the most likely candidate gene for chieh-qua gynoecy, which differed from the reported gynoecious genes.
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spelling pubmed-101571662023-05-05 Fine mapping and candidate gene analysis of gynoecy trait in chieh-qua (Benincasa hispida Cogn. var. chieh-qua How) Wang, Min Yang, Songguang Liu, Wei Cao, Zhenqiang Chen, Lin Liu, Wenrui Xie, Dasen Yan, Jinqiang Jiang, Biao Peng, Qingwu Front Plant Sci Plant Science Gynoecy demonstrates an earlier production of hybrids and a higher yield and improves the efficiency of hybrid seed production. Therefore, the utilization of gynoecy is beneficial for the genetic breeding of chieh-qua. However, little knowledge of gynoecious-related genes in chieh-qua has been reported until now. Here, we used an F(2) population from the cross between the gynoecious line ‘A36’ and the monoecious line ‘SX’ for genetic mapping and revealed that chieh-qua gynoecy was regulated by a single recessive gene. We fine-mapped it into a 530-kb region flanked by the markers Indel-3 and KASP145 on Chr.8, which harbors eight candidate genes. One of the candidate genes, Bhi08G000345, encoding networked protein 4 (CqNET4), contained a non-synonymous SNP resulting in the amino acid substitution of isoleucine (ATA; I) to methionine (ATG; M). CqNET4 was prominently expressed in the female flower, and only three genes related to ethylene synthesis were significantly expressed between ‘A36’ and ‘SX.’ The results presented here provide support for the CqNET4 as the most likely candidate gene for chieh-qua gynoecy, which differed from the reported gynoecious genes. Frontiers Media S.A. 2023-04-20 /pmc/articles/PMC10157166/ /pubmed/37152167 http://dx.doi.org/10.3389/fpls.2023.1158735 Text en Copyright © 2023 Wang, Yang, Liu, Cao, Chen, Liu, Xie, Yan, Jiang and Peng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wang, Min
Yang, Songguang
Liu, Wei
Cao, Zhenqiang
Chen, Lin
Liu, Wenrui
Xie, Dasen
Yan, Jinqiang
Jiang, Biao
Peng, Qingwu
Fine mapping and candidate gene analysis of gynoecy trait in chieh-qua (Benincasa hispida Cogn. var. chieh-qua How)
title Fine mapping and candidate gene analysis of gynoecy trait in chieh-qua (Benincasa hispida Cogn. var. chieh-qua How)
title_full Fine mapping and candidate gene analysis of gynoecy trait in chieh-qua (Benincasa hispida Cogn. var. chieh-qua How)
title_fullStr Fine mapping and candidate gene analysis of gynoecy trait in chieh-qua (Benincasa hispida Cogn. var. chieh-qua How)
title_full_unstemmed Fine mapping and candidate gene analysis of gynoecy trait in chieh-qua (Benincasa hispida Cogn. var. chieh-qua How)
title_short Fine mapping and candidate gene analysis of gynoecy trait in chieh-qua (Benincasa hispida Cogn. var. chieh-qua How)
title_sort fine mapping and candidate gene analysis of gynoecy trait in chieh-qua (benincasa hispida cogn. var. chieh-qua how)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10157166/
https://www.ncbi.nlm.nih.gov/pubmed/37152167
http://dx.doi.org/10.3389/fpls.2023.1158735
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