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Hybridization alters the gut microbial and metabolic profile concurrent with modifying intestinal functions in Tunchang pigs

INTRODUCTION: Hybridization has been widely used among Chinese wild boars to improve their growth performance and maintain meat quality. Most studies have focused on the genetic basis for such variation. However, the differences in the gut environment between hybrid and purebred boars, which can hav...

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Autores principales: He, Jiayi, Zhang, Yunchao, Li, Hui, Xie, Yanshe, Huang, Guiqing, Peng, Chen, Zhao, Pengju, Wang, Zhengguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10157192/
https://www.ncbi.nlm.nih.gov/pubmed/37152756
http://dx.doi.org/10.3389/fmicb.2023.1159653
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author He, Jiayi
Zhang, Yunchao
Li, Hui
Xie, Yanshe
Huang, Guiqing
Peng, Chen
Zhao, Pengju
Wang, Zhengguang
author_facet He, Jiayi
Zhang, Yunchao
Li, Hui
Xie, Yanshe
Huang, Guiqing
Peng, Chen
Zhao, Pengju
Wang, Zhengguang
author_sort He, Jiayi
collection PubMed
description INTRODUCTION: Hybridization has been widely used among Chinese wild boars to improve their growth performance and maintain meat quality. Most studies have focused on the genetic basis for such variation. However, the differences in the gut environment between hybrid and purebred boars, which can have significant impacts on their health and productivity, have been poorly understood. METHODS: In the current study, metagenomics was used to detect the gut microbial diversity and composition in hybrid Batun (BT, Berkshire × Tunchang) pigs and purebred Tunchang (TC) pigs. Additionally, untargeted metabolomic analysis was used to detect differences in gut metabolic pathways. Furthermore, multiple molecular experiments were conducted to demonstrate differences in intestinal functions. RESULTS: As a result of hybridization in TC pigs, a microbial change was observed, especially in Prevotella and Lactobacillus. Significant differences were found in gut metabolites, including fatty acyls, steroids, and steroid derivatives. Furthermore, the function of the intestinal barrier was decreased by hybridization, while the function of nutrient metabolism was increased. DISCUSSION: Evidences were shown that hybridization changed the gut microbiome, gut metabolome, and intestinal functions of TC pigs. These findings supported our hypothesis that hybridization altered the gut microbial composition, thereby modifying the intestinal functions, even the host phenotypes. Overall, our study highlights the importance of considering the gut microbiome as a key factor in the evaluation of animal health and productivity, particularly in the context of genetic selection and breeding programs.
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spelling pubmed-101571922023-05-05 Hybridization alters the gut microbial and metabolic profile concurrent with modifying intestinal functions in Tunchang pigs He, Jiayi Zhang, Yunchao Li, Hui Xie, Yanshe Huang, Guiqing Peng, Chen Zhao, Pengju Wang, Zhengguang Front Microbiol Microbiology INTRODUCTION: Hybridization has been widely used among Chinese wild boars to improve their growth performance and maintain meat quality. Most studies have focused on the genetic basis for such variation. However, the differences in the gut environment between hybrid and purebred boars, which can have significant impacts on their health and productivity, have been poorly understood. METHODS: In the current study, metagenomics was used to detect the gut microbial diversity and composition in hybrid Batun (BT, Berkshire × Tunchang) pigs and purebred Tunchang (TC) pigs. Additionally, untargeted metabolomic analysis was used to detect differences in gut metabolic pathways. Furthermore, multiple molecular experiments were conducted to demonstrate differences in intestinal functions. RESULTS: As a result of hybridization in TC pigs, a microbial change was observed, especially in Prevotella and Lactobacillus. Significant differences were found in gut metabolites, including fatty acyls, steroids, and steroid derivatives. Furthermore, the function of the intestinal barrier was decreased by hybridization, while the function of nutrient metabolism was increased. DISCUSSION: Evidences were shown that hybridization changed the gut microbiome, gut metabolome, and intestinal functions of TC pigs. These findings supported our hypothesis that hybridization altered the gut microbial composition, thereby modifying the intestinal functions, even the host phenotypes. Overall, our study highlights the importance of considering the gut microbiome as a key factor in the evaluation of animal health and productivity, particularly in the context of genetic selection and breeding programs. Frontiers Media S.A. 2023-04-20 /pmc/articles/PMC10157192/ /pubmed/37152756 http://dx.doi.org/10.3389/fmicb.2023.1159653 Text en Copyright © 2023 He, Zhang, Li, Xie, Huang, Peng, Zhao and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
He, Jiayi
Zhang, Yunchao
Li, Hui
Xie, Yanshe
Huang, Guiqing
Peng, Chen
Zhao, Pengju
Wang, Zhengguang
Hybridization alters the gut microbial and metabolic profile concurrent with modifying intestinal functions in Tunchang pigs
title Hybridization alters the gut microbial and metabolic profile concurrent with modifying intestinal functions in Tunchang pigs
title_full Hybridization alters the gut microbial and metabolic profile concurrent with modifying intestinal functions in Tunchang pigs
title_fullStr Hybridization alters the gut microbial and metabolic profile concurrent with modifying intestinal functions in Tunchang pigs
title_full_unstemmed Hybridization alters the gut microbial and metabolic profile concurrent with modifying intestinal functions in Tunchang pigs
title_short Hybridization alters the gut microbial and metabolic profile concurrent with modifying intestinal functions in Tunchang pigs
title_sort hybridization alters the gut microbial and metabolic profile concurrent with modifying intestinal functions in tunchang pigs
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10157192/
https://www.ncbi.nlm.nih.gov/pubmed/37152756
http://dx.doi.org/10.3389/fmicb.2023.1159653
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