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Chronic larval exposure to thiacloprid impairs honeybee antennal selectivity, learning and memory performances

The use of agricultural neonicotinoid insecticides has sub-lethal chronic effects on bees that are more prevalent than acute toxicity. Among these insecticides, thiacloprid, a commonly used compound with low toxicity, has attracted significant attention due to its potential impact on the olfactory a...

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Autores principales: Ke, Li, Chen, Xiasang, Dai, Pingli, Liu, Yong-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10157261/
https://www.ncbi.nlm.nih.gov/pubmed/37153228
http://dx.doi.org/10.3389/fphys.2023.1114488
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author Ke, Li
Chen, Xiasang
Dai, Pingli
Liu, Yong-Jun
author_facet Ke, Li
Chen, Xiasang
Dai, Pingli
Liu, Yong-Jun
author_sort Ke, Li
collection PubMed
description The use of agricultural neonicotinoid insecticides has sub-lethal chronic effects on bees that are more prevalent than acute toxicity. Among these insecticides, thiacloprid, a commonly used compound with low toxicity, has attracted significant attention due to its potential impact on the olfactory and learning abilities of honeybees. The effect of sub-lethal larval exposure to thiacloprid on the antennal activity of adult honeybees (Apis mellifera L.) is not yet fully understood. To address this knowledge gap, laboratory-based experiments were conducted in which honeybee larvae were administered thiacloprid (0.5 mg/L and 1.0 mg/L). Using electroantennography (EAG), the impacts of thiacloprid exposure on the antennal selectivity to common floral volatiles were evaluated. Additionally, the effects of sub-lethal exposure on odor-related learning and memory were also assessed. The results of this study reveal, for the first time, that sub-lethal larval exposure to thiacloprid decreased honeybee antenna EAG responses to floral scents, leading to increased olfactory selectivity in the high-dose (1.0 mg/L) group compared to the control group (0 mg/L vs. 1.0 mg/L: p = 0.042). The results also suggest that thiacloprid negatively affected odor-associated paired learning acquisition, as well as medium-term (1 h) (0 mg/L vs. 1.0 mg/L: p = 0.019) and long-term memory (24 h) (0 mg/L vs. 1.0 mg/L: p = 0.037) in adult honeybees. EAG amplitudes were dramatically reduced following R-linalool paired olfactory training (0 mg/L vs. 1.0 mg/L: p = 0.001; 0 mg/L vs. 0.5 mg/L: p = 0.027), while antennal activities only differed significantly in the control between paired and unpaired groups. Our results indicated that exposure to sub-lethal concentrations of thiacloprid may affect olfactory perception and learning and memory behaviors in honeybees. These findings have important implications for the safe use of agrochemicals in the environment.
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spelling pubmed-101572612023-05-05 Chronic larval exposure to thiacloprid impairs honeybee antennal selectivity, learning and memory performances Ke, Li Chen, Xiasang Dai, Pingli Liu, Yong-Jun Front Physiol Physiology The use of agricultural neonicotinoid insecticides has sub-lethal chronic effects on bees that are more prevalent than acute toxicity. Among these insecticides, thiacloprid, a commonly used compound with low toxicity, has attracted significant attention due to its potential impact on the olfactory and learning abilities of honeybees. The effect of sub-lethal larval exposure to thiacloprid on the antennal activity of adult honeybees (Apis mellifera L.) is not yet fully understood. To address this knowledge gap, laboratory-based experiments were conducted in which honeybee larvae were administered thiacloprid (0.5 mg/L and 1.0 mg/L). Using electroantennography (EAG), the impacts of thiacloprid exposure on the antennal selectivity to common floral volatiles were evaluated. Additionally, the effects of sub-lethal exposure on odor-related learning and memory were also assessed. The results of this study reveal, for the first time, that sub-lethal larval exposure to thiacloprid decreased honeybee antenna EAG responses to floral scents, leading to increased olfactory selectivity in the high-dose (1.0 mg/L) group compared to the control group (0 mg/L vs. 1.0 mg/L: p = 0.042). The results also suggest that thiacloprid negatively affected odor-associated paired learning acquisition, as well as medium-term (1 h) (0 mg/L vs. 1.0 mg/L: p = 0.019) and long-term memory (24 h) (0 mg/L vs. 1.0 mg/L: p = 0.037) in adult honeybees. EAG amplitudes were dramatically reduced following R-linalool paired olfactory training (0 mg/L vs. 1.0 mg/L: p = 0.001; 0 mg/L vs. 0.5 mg/L: p = 0.027), while antennal activities only differed significantly in the control between paired and unpaired groups. Our results indicated that exposure to sub-lethal concentrations of thiacloprid may affect olfactory perception and learning and memory behaviors in honeybees. These findings have important implications for the safe use of agrochemicals in the environment. Frontiers Media S.A. 2023-04-20 /pmc/articles/PMC10157261/ /pubmed/37153228 http://dx.doi.org/10.3389/fphys.2023.1114488 Text en Copyright © 2023 Ke, Chen, Dai and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Ke, Li
Chen, Xiasang
Dai, Pingli
Liu, Yong-Jun
Chronic larval exposure to thiacloprid impairs honeybee antennal selectivity, learning and memory performances
title Chronic larval exposure to thiacloprid impairs honeybee antennal selectivity, learning and memory performances
title_full Chronic larval exposure to thiacloprid impairs honeybee antennal selectivity, learning and memory performances
title_fullStr Chronic larval exposure to thiacloprid impairs honeybee antennal selectivity, learning and memory performances
title_full_unstemmed Chronic larval exposure to thiacloprid impairs honeybee antennal selectivity, learning and memory performances
title_short Chronic larval exposure to thiacloprid impairs honeybee antennal selectivity, learning and memory performances
title_sort chronic larval exposure to thiacloprid impairs honeybee antennal selectivity, learning and memory performances
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10157261/
https://www.ncbi.nlm.nih.gov/pubmed/37153228
http://dx.doi.org/10.3389/fphys.2023.1114488
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