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pPe Op inhibits HGC-27 cell proliferation, migration and invasion by upregulating miR-30b-5p and down-regulating the Rac1/Cdc42 pathway : pPe Op inhibits gastric cancer via upregulating miR-30b-5p

Gastric cancer is the fifth most frequently occurring and the fourth most lethal malignant cancer worldwide. A bioactive protein (pPe Op) from Omphalia lapidescens exhibits significant inhibitory effects on gastric cancer cells. miRNA deep sequencing analysis shows that miR-30b-5p is significantly u...

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Autores principales: Xu, Wenjun, Fu, Zhenjie, Xu, Yuqin, Cheung, Man Hei, Chen, Yan, Lin, Meiai, Wen, Hang, Lv, Hang, Liang, Chun, Lou, Jianshu, Chen, Yitao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10157518/
https://www.ncbi.nlm.nih.gov/pubmed/36789688
http://dx.doi.org/10.3724/abbs.2022193
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author Xu, Wenjun
Fu, Zhenjie
Xu, Yuqin
Cheung, Man Hei
Chen, Yan
Lin, Meiai
Wen, Hang
Lv, Hang
Liang, Chun
Lou, Jianshu
Chen, Yitao
author_facet Xu, Wenjun
Fu, Zhenjie
Xu, Yuqin
Cheung, Man Hei
Chen, Yan
Lin, Meiai
Wen, Hang
Lv, Hang
Liang, Chun
Lou, Jianshu
Chen, Yitao
author_sort Xu, Wenjun
collection PubMed
description Gastric cancer is the fifth most frequently occurring and the fourth most lethal malignant cancer worldwide. A bioactive protein (pPe Op) from Omphalia lapidescens exhibits significant inhibitory effects on gastric cancer cells. miRNA deep sequencing analysis shows that miR-30b-5p is significantly upregulated in HGC-27 cells treated with pPe Op. Verification results show that the expression level of miR-30b-5p is significantly increased in HGC-27 cells after pPe Op treatment. Additionally, miR-30b-5p is significantly downregulated in clinical gastric cancer tissues compared to that in adjacent normal tissues. Following pPe Op treatment and/or transfection with miR-30b-5p mimic, the proliferation, migration, and invasion of HGC-27 cells are significantly impaired. Immunofluorescence microscopy shows that pPe Op and/or miR-30b-5p destroy(s) microfilaments and microstructures and inhibit(s) the formation of pseudopodia. Bioinformatics analysis, dual-luciferase reporter assay, and western blot analysis confirm that miR-30b-5p downregulates Rac1/Cdc42 expression and activation by targeting RAB22A. Available data indicate that miR-30b-5p plays an anti-gastric cancer role in mediating pPe Op. pPe Op upregulates miR-30b-5p expression, which in turn inhibits RAB22A expression, resulting in a reduction in the expression and activation of Rac1 and Cdc42 and their downstream targets, thus destroying the cytoskeletal structure and inhibiting the proliferation, migration, and invasion of cancer cells.
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spelling pubmed-101575182023-05-05 pPe Op inhibits HGC-27 cell proliferation, migration and invasion by upregulating miR-30b-5p and down-regulating the Rac1/Cdc42 pathway : pPe Op inhibits gastric cancer via upregulating miR-30b-5p Xu, Wenjun Fu, Zhenjie Xu, Yuqin Cheung, Man Hei Chen, Yan Lin, Meiai Wen, Hang Lv, Hang Liang, Chun Lou, Jianshu Chen, Yitao Acta Biochim Biophys Sin (Shanghai) Research Article Gastric cancer is the fifth most frequently occurring and the fourth most lethal malignant cancer worldwide. A bioactive protein (pPe Op) from Omphalia lapidescens exhibits significant inhibitory effects on gastric cancer cells. miRNA deep sequencing analysis shows that miR-30b-5p is significantly upregulated in HGC-27 cells treated with pPe Op. Verification results show that the expression level of miR-30b-5p is significantly increased in HGC-27 cells after pPe Op treatment. Additionally, miR-30b-5p is significantly downregulated in clinical gastric cancer tissues compared to that in adjacent normal tissues. Following pPe Op treatment and/or transfection with miR-30b-5p mimic, the proliferation, migration, and invasion of HGC-27 cells are significantly impaired. Immunofluorescence microscopy shows that pPe Op and/or miR-30b-5p destroy(s) microfilaments and microstructures and inhibit(s) the formation of pseudopodia. Bioinformatics analysis, dual-luciferase reporter assay, and western blot analysis confirm that miR-30b-5p downregulates Rac1/Cdc42 expression and activation by targeting RAB22A. Available data indicate that miR-30b-5p plays an anti-gastric cancer role in mediating pPe Op. pPe Op upregulates miR-30b-5p expression, which in turn inhibits RAB22A expression, resulting in a reduction in the expression and activation of Rac1 and Cdc42 and their downstream targets, thus destroying the cytoskeletal structure and inhibiting the proliferation, migration, and invasion of cancer cells. Oxford University Press 2022-12-20 /pmc/articles/PMC10157518/ /pubmed/36789688 http://dx.doi.org/10.3724/abbs.2022193 Text en © The Author(s) 2021. 0 https://creativecommons.org/licenses/by-nc/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/).
spellingShingle Research Article
Xu, Wenjun
Fu, Zhenjie
Xu, Yuqin
Cheung, Man Hei
Chen, Yan
Lin, Meiai
Wen, Hang
Lv, Hang
Liang, Chun
Lou, Jianshu
Chen, Yitao
pPe Op inhibits HGC-27 cell proliferation, migration and invasion by upregulating miR-30b-5p and down-regulating the Rac1/Cdc42 pathway : pPe Op inhibits gastric cancer via upregulating miR-30b-5p
title pPe Op inhibits HGC-27 cell proliferation, migration and invasion by upregulating miR-30b-5p and down-regulating the Rac1/Cdc42 pathway : pPe Op inhibits gastric cancer via upregulating miR-30b-5p
title_full pPe Op inhibits HGC-27 cell proliferation, migration and invasion by upregulating miR-30b-5p and down-regulating the Rac1/Cdc42 pathway : pPe Op inhibits gastric cancer via upregulating miR-30b-5p
title_fullStr pPe Op inhibits HGC-27 cell proliferation, migration and invasion by upregulating miR-30b-5p and down-regulating the Rac1/Cdc42 pathway : pPe Op inhibits gastric cancer via upregulating miR-30b-5p
title_full_unstemmed pPe Op inhibits HGC-27 cell proliferation, migration and invasion by upregulating miR-30b-5p and down-regulating the Rac1/Cdc42 pathway : pPe Op inhibits gastric cancer via upregulating miR-30b-5p
title_short pPe Op inhibits HGC-27 cell proliferation, migration and invasion by upregulating miR-30b-5p and down-regulating the Rac1/Cdc42 pathway : pPe Op inhibits gastric cancer via upregulating miR-30b-5p
title_sort ppe op inhibits hgc-27 cell proliferation, migration and invasion by upregulating mir-30b-5p and down-regulating the rac1/cdc42 pathway : ppe op inhibits gastric cancer via upregulating mir-30b-5p
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10157518/
https://www.ncbi.nlm.nih.gov/pubmed/36789688
http://dx.doi.org/10.3724/abbs.2022193
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