Cargando…

DNA extraction protocol impacts ocular surface microbiome profile

PURPOSE: The aim of this study is to provide a reference frame to allow the comparison and interpretation of currently published studies on 16S ribosomal ribonucleic acid amplicon sequencing of ocular microbiome samples using different DNA extraction protocols. Alongside, the quantitative and qualit...

Descripción completa

Detalles Bibliográficos
Autores principales:	Delbeke, Heleen, Casteels, Ingele, Joossens, Marie
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2023
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10157640/ https://www.ncbi.nlm.nih.gov/pubmed/37152736 http://dx.doi.org/10.3389/fmicb.2023.1128917

Ejemplares similares

The Effect of Topical Anesthetics on 16S Ribosomal Ribonucleic Acid Amplicon Sequencing Results in Ocular Surface Microbiome Research
por: Delbeke, Heleen, et al.
Publicado: (2022)

Corrigendum to “In search of viable SARS-CoV-2 in the tear film: a prospective clinical study in hospitalized symptomatic patients” [Clin Microbiol Infect 28(8) (2022 Aug) 1172–1173]
por: Leysen, Laura, et al.
Publicado: (2023)

In search of viable SARS-CoV-2 in the tear film: a prospective clinical study in hospitalized symptomatic patients
por: Leysen, Laura, et al.
Publicado: (2022)

Metagenomic Profiling of Ocular Surface Microbiome Changes in Meibomian Gland Dysfunction
por: Zhao, Fuxin, et al.
Publicado: (2020)

Metagenomic profiling of ocular surface microbiome changes in Demodex blepharitis patients
por: Fu, Yana, et al.
Publicado: (2022)

The Microbiome of the Meibum and Ocular Surface in Healthy Subjects
por: Suzuki, Tomo, et al.
Publicado: (2020)

Alterations in the Ocular Surface Microbiome in Traumatic Corneal Ulcer Patients
por: Kang, Yutong, et al.
Publicado: (2020)

Understanding the Interactions Between the Ocular Surface Microbiome and the Tear Proteome
por: Zysset-Burri, Denise C., et al.
Publicado: (2021)

Topical Glaucoma Therapy Is Associated With Alterations of the Ocular Surface Microbiome
por: Chang, Chih-Chiun J., et al.
Publicado: (2022)

Exudative Type 3 Retinal Arteriovenous Malformation in a Pediatric Patient
por: Dens, Helena, et al.
Publicado: (2018)

Disturbances in the ocular surface microbiome by perioperative antimicrobial eye drops
por: Hotta, Fumika, et al.
Publicado: (2023)

Challenges and insights in the exploration of the low abundance human ocular surface microbiome
por: Herzog, Elio L., et al.
Publicado: (2023)

Comparison of Meconium DNA Extraction Methods for Use in Microbiome Studies
por: Stinson, Lisa F., et al.
Publicado: (2018)

Veillonella parvula periorbital cellulitis: an unusual pathogen causing a common clinical sign
por: Wellens, Liesbeth, et al.
Publicado: (2019)

Bilateral simultaneous anterior ischemic optic neuropathy, an extrahepatic manifestation of hepatitis C cured with direct acting antivirals
por: Prud’homme, Sylvie, et al.
Publicado: (2016)

Ocular surface microbiome in diabetes mellitus
por: Suwajanakorn, Orathai, et al.
Publicado: (2022)

Analyses of the Bacterial Contamination on Belgian Broiler Carcasses at Retail Level
por: Yu, Zhongjia, et al.
Publicado: (2020)

The saliva microbiome profiles are minimally affected by collection method or DNA extraction protocols
por: Lim, Yenkai, et al.
Publicado: (2017)

Ocular surface microbiota: Ophthalmic infectious disease and probiotics
por: Chiang, Ming-Cheng, et al.
Publicado: (2022)

Ophthalmological and electrophysiological findings in monozygotic twin sisters with phosphomannomutase 2 deficiency (PMM2-CDG) over a period of 37 years
por: Van Hees, Ines, et al.
Publicado: (2019)

Subconjunctival Hemorrhage in a Child with the Blue Rubber Bleb Nevus Syndrome on Treatment with Oral Propranolol
por: De Loecker, Karen, et al.
Publicado: (2021)

Genetic Manipulation of Corynebacterium mastitidis to Better Understand the Ocular Microbiome
por: Rigas, Yannis, et al.
Publicado: (2023)

Conjunctival sac microbiome in anophthalmic patients: Flora diversity and the impact of ocular prosthesis materials
por: Zhao, Hejia, et al.
Publicado: (2023)

Categorization of the Ocular Microbiome in Japanese Stevens–Johnson Syndrome Patients With Severe Ocular Complications
por: Ueta, Mayumi, et al.
Publicado: (2021)

Methodologic Considerations for Studying the Ocular Surface Microbiome
por: Chowdhary, Apoorva, et al.
Publicado: (2023)

Role of ocular surface microbiome in health and disease
por: Doularamani, Minal, et al.
Publicado: (2023)

Anatomic Characterization of the Ocular Surface Microbiome in Children
por: Cavuoto, Kara M., et al.
Publicado: (2019)

Temporal Stability and Composition of the Ocular Surface Microbiome
por: Ozkan, Jerome, et al.
Publicado: (2017)

Characterization and Comparison of Ocular Surface Microbiome in Newborns
por: Petrillo, Francesco, et al.
Publicado: (2022)

Bacterial DNA on the skin surface overrepresents the viable skin microbiome
por: Acosta, Ellen M, et al.
Publicado: (2023)

Optical Coherence Tomography Angiography of Retinal Microvascular Changes Overlying Choroidal Nodules in Neurofibromatosis Type 1
por: Cassiman, Catherine, et al.
Publicado: (2017)

Clinical Practice: A Proposed Standardized Ophthalmological Assessment for Patients with Cystinosis
por: Pinxten, Anne-Marie, et al.
Publicado: (2017)

Impact of Host DNA and Sequencing Depth on the Taxonomic Resolution of Whole Metagenome Sequencing for Microbiome Analysis
por: Pereira-Marques, Joana, et al.
Publicado: (2019)

Impact of DNA Extraction Method on Variation in Human and Built Environment Microbial Community and Functional Profiles Assessed by Shotgun Metagenomics Sequencing
por: Sui, Hui-yu, et al.
Publicado: (2020)

Gut and lung microbiome profiles in pregnant mice
por: Wiscovitch-Russo, Rosana, et al.
Publicado: (2022)

Antimicrobial Peptide Expression at the Ocular Surface and Their Therapeutic Use in the Treatment of Microbial Keratitis
por: Shannon, Allison H., et al.
Publicado: (2022)

Strain heterogeneity, cooccurrence network, taxonomic composition and functional profile of the healthy ocular surface microbiome
por: Kang, Yutong, et al.
Publicado: (2021)

Landscape characteristics shape surface soil microbiomes in the Chihuahuan Desert
por: Hansen, Frederick A., et al.
Publicado: (2023)

Diversity and Selection of Surface Marine Microbiomes in the Atlantic-Influenced Arctic
por: Aalto, Nerea J., et al.
Publicado: (2022)

Cyprus Sausages’ Bacterial Community Identification Through Metataxonomic Sequencing: Evaluation of the Impact of Different DNA Extraction Protocols on the Sausages’ Microbial Diversity Representation
por: Kamilari, Eleni, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_chiv82b3dukb18s8t1ai2gt7bh