Cargando…

Characterization and Application of a Synthesized Novel Poly(chlorobis(1,10-phenanthroline)resorcinolcobalt(II) chloride)-Modified Glassy Carbon Electrode for Selective Voltammetric Determination of Cefadroxil in Pharmaceutical Formulations, Human Urine, and Blood Serum Samples

[Image: see text] Cefadroxil belongs to the β-lactam antibiotics, mainly used for the treatment of various bacterial infections, caused by Gram-positive and Gram-negative bacteria. However, it is also encountering serious bacterial resistance, necessitating continuous monitoring of its level in phar...

Descripción completa

Detalles Bibliográficos
Autores principales: Kassa, Adane, Benor, Amare, Tigineh, Getinet Tamiru, Abebe, Atakilt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10157687/
https://www.ncbi.nlm.nih.gov/pubmed/37151555
http://dx.doi.org/10.1021/acsomega.3c00170
_version_ 1785036807596933120
author Kassa, Adane
Benor, Amare
Tigineh, Getinet Tamiru
Abebe, Atakilt
author_facet Kassa, Adane
Benor, Amare
Tigineh, Getinet Tamiru
Abebe, Atakilt
author_sort Kassa, Adane
collection PubMed
description [Image: see text] Cefadroxil belongs to the β-lactam antibiotics, mainly used for the treatment of various bacterial infections, caused by Gram-positive and Gram-negative bacteria. However, it is also encountering serious bacterial resistance, necessitating continuous monitoring of its level in pharmaceutical and biological samples. This study presents a selective, accurate, and precise square-wave voltammetric method based on a novel poly(chlorobis(1,10-phenanthroline)resorcinolcobalt(II)chloride)-modified glassy carbon electrode (poly(CP(2)RCoC)/GCE) for determination of cefadroxil (CDL). UV–vis spectroscopy, FT-IR spectroscopy, metal and halide estimation, CHN elemental analysis, and electrolytic conductivity measurement results confirmed the synthesis of the title complex modifier. Electrode characterization results revealed modification of the surface of the electrode by an electroactive and a conductive polymer film (poly(CP(2)RCoC)/GCE), leading to an improved effective electrode surface area. In contrast to the bare electrode, the appearance of an irreversible oxidative peak at a much reduced potential with a 7-fold current enhancement at poly(CP(2)RCoC)/GCE showed the catalytic effect of the modifier toward oxidation of CDL. The square-wave voltammetric current response of poly(CP(2)RCoC)/GCE showed a linear dependence on the concentration of CDL in the range of 1 × 10(–7)–3.0 × 10(–4) M with a detection limit of 4.3 × 10(–9). The CDL level in the selected two tablet brands was in the range of 97.25–100.00% of their labeled values. The spike recovery results in tablet, human blood serum, and urine samples were 98.85–101.30, 99.20–100.39, and 98.10–99.99%, respectively. Interference recovery results with a less than 4.74% error, lower LoD, and wider dynamic range than the previously reported methods validated the potential applicability of the present method with excellent accuracy and sensitivity based on the novel mixed-ligand complex-modified GCE (poly(CP(2)RCoC)/GCE) for determination of CDL in various real samples with a complex matrix.
format Online
Article
Text
id pubmed-10157687
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-101576872023-05-05 Characterization and Application of a Synthesized Novel Poly(chlorobis(1,10-phenanthroline)resorcinolcobalt(II) chloride)-Modified Glassy Carbon Electrode for Selective Voltammetric Determination of Cefadroxil in Pharmaceutical Formulations, Human Urine, and Blood Serum Samples Kassa, Adane Benor, Amare Tigineh, Getinet Tamiru Abebe, Atakilt ACS Omega [Image: see text] Cefadroxil belongs to the β-lactam antibiotics, mainly used for the treatment of various bacterial infections, caused by Gram-positive and Gram-negative bacteria. However, it is also encountering serious bacterial resistance, necessitating continuous monitoring of its level in pharmaceutical and biological samples. This study presents a selective, accurate, and precise square-wave voltammetric method based on a novel poly(chlorobis(1,10-phenanthroline)resorcinolcobalt(II)chloride)-modified glassy carbon electrode (poly(CP(2)RCoC)/GCE) for determination of cefadroxil (CDL). UV–vis spectroscopy, FT-IR spectroscopy, metal and halide estimation, CHN elemental analysis, and electrolytic conductivity measurement results confirmed the synthesis of the title complex modifier. Electrode characterization results revealed modification of the surface of the electrode by an electroactive and a conductive polymer film (poly(CP(2)RCoC)/GCE), leading to an improved effective electrode surface area. In contrast to the bare electrode, the appearance of an irreversible oxidative peak at a much reduced potential with a 7-fold current enhancement at poly(CP(2)RCoC)/GCE showed the catalytic effect of the modifier toward oxidation of CDL. The square-wave voltammetric current response of poly(CP(2)RCoC)/GCE showed a linear dependence on the concentration of CDL in the range of 1 × 10(–7)–3.0 × 10(–4) M with a detection limit of 4.3 × 10(–9). The CDL level in the selected two tablet brands was in the range of 97.25–100.00% of their labeled values. The spike recovery results in tablet, human blood serum, and urine samples were 98.85–101.30, 99.20–100.39, and 98.10–99.99%, respectively. Interference recovery results with a less than 4.74% error, lower LoD, and wider dynamic range than the previously reported methods validated the potential applicability of the present method with excellent accuracy and sensitivity based on the novel mixed-ligand complex-modified GCE (poly(CP(2)RCoC)/GCE) for determination of CDL in various real samples with a complex matrix. American Chemical Society 2023-04-18 /pmc/articles/PMC10157687/ /pubmed/37151555 http://dx.doi.org/10.1021/acsomega.3c00170 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Kassa, Adane
Benor, Amare
Tigineh, Getinet Tamiru
Abebe, Atakilt
Characterization and Application of a Synthesized Novel Poly(chlorobis(1,10-phenanthroline)resorcinolcobalt(II) chloride)-Modified Glassy Carbon Electrode for Selective Voltammetric Determination of Cefadroxil in Pharmaceutical Formulations, Human Urine, and Blood Serum Samples
title Characterization and Application of a Synthesized Novel Poly(chlorobis(1,10-phenanthroline)resorcinolcobalt(II) chloride)-Modified Glassy Carbon Electrode for Selective Voltammetric Determination of Cefadroxil in Pharmaceutical Formulations, Human Urine, and Blood Serum Samples
title_full Characterization and Application of a Synthesized Novel Poly(chlorobis(1,10-phenanthroline)resorcinolcobalt(II) chloride)-Modified Glassy Carbon Electrode for Selective Voltammetric Determination of Cefadroxil in Pharmaceutical Formulations, Human Urine, and Blood Serum Samples
title_fullStr Characterization and Application of a Synthesized Novel Poly(chlorobis(1,10-phenanthroline)resorcinolcobalt(II) chloride)-Modified Glassy Carbon Electrode for Selective Voltammetric Determination of Cefadroxil in Pharmaceutical Formulations, Human Urine, and Blood Serum Samples
title_full_unstemmed Characterization and Application of a Synthesized Novel Poly(chlorobis(1,10-phenanthroline)resorcinolcobalt(II) chloride)-Modified Glassy Carbon Electrode for Selective Voltammetric Determination of Cefadroxil in Pharmaceutical Formulations, Human Urine, and Blood Serum Samples
title_short Characterization and Application of a Synthesized Novel Poly(chlorobis(1,10-phenanthroline)resorcinolcobalt(II) chloride)-Modified Glassy Carbon Electrode for Selective Voltammetric Determination of Cefadroxil in Pharmaceutical Formulations, Human Urine, and Blood Serum Samples
title_sort characterization and application of a synthesized novel poly(chlorobis(1,10-phenanthroline)resorcinolcobalt(ii) chloride)-modified glassy carbon electrode for selective voltammetric determination of cefadroxil in pharmaceutical formulations, human urine, and blood serum samples
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10157687/
https://www.ncbi.nlm.nih.gov/pubmed/37151555
http://dx.doi.org/10.1021/acsomega.3c00170
work_keys_str_mv AT kassaadane characterizationandapplicationofasynthesizednovelpolychlorobis110phenanthrolineresorcinolcobaltiichloridemodifiedglassycarbonelectrodeforselectivevoltammetricdeterminationofcefadroxilinpharmaceuticalformulationshumanurineandbloodserumsamples
AT benoramare characterizationandapplicationofasynthesizednovelpolychlorobis110phenanthrolineresorcinolcobaltiichloridemodifiedglassycarbonelectrodeforselectivevoltammetricdeterminationofcefadroxilinpharmaceuticalformulationshumanurineandbloodserumsamples
AT tiginehgetinettamiru characterizationandapplicationofasynthesizednovelpolychlorobis110phenanthrolineresorcinolcobaltiichloridemodifiedglassycarbonelectrodeforselectivevoltammetricdeterminationofcefadroxilinpharmaceuticalformulationshumanurineandbloodserumsamples
AT abebeatakilt characterizationandapplicationofasynthesizednovelpolychlorobis110phenanthrolineresorcinolcobaltiichloridemodifiedglassycarbonelectrodeforselectivevoltammetricdeterminationofcefadroxilinpharmaceuticalformulationshumanurineandbloodserumsamples