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Highly efficient and rapid generation of genetic variants
A new mutagenesis platform enables the fast, cost-efficient and automatable production of defined multi-site sequence variants for a wide range of applications. Demonstrations of this method included the generation of SARS-CoV-2 spike gene variants, DNA fragments for large-scale genome engineering,...
| Formato: | Online Artículo Texto |
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| Lenguaje: | English |
| Publicado: |
Nature Publishing Group US
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10158673/ https://www.ncbi.nlm.nih.gov/pubmed/37142768 http://dx.doi.org/10.1038/s41592-023-01869-0 |
| _version_ | 1785036977922375680 |
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| collection | PubMed |
| description | A new mutagenesis platform enables the fast, cost-efficient and automatable production of defined multi-site sequence variants for a wide range of applications. Demonstrations of this method included the generation of SARS-CoV-2 spike gene variants, DNA fragments for large-scale genome engineering, and adeno-associated virus 2 (AAV2) cap genes with improved packaging capacity. |
| format | Online Article Text |
| id | pubmed-10158673 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | Nature Publishing Group US |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-101586732023-05-09 Highly efficient and rapid generation of genetic variants Nat Methods Research Briefing A new mutagenesis platform enables the fast, cost-efficient and automatable production of defined multi-site sequence variants for a wide range of applications. Demonstrations of this method included the generation of SARS-CoV-2 spike gene variants, DNA fragments for large-scale genome engineering, and adeno-associated virus 2 (AAV2) cap genes with improved packaging capacity. Nature Publishing Group US 2023-05-04 2023 /pmc/articles/PMC10158673/ /pubmed/37142768 http://dx.doi.org/10.1038/s41592-023-01869-0 Text en © Springer Nature America, Inc. 2023 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
| spellingShingle | Research Briefing Highly efficient and rapid generation of genetic variants |
| title | Highly efficient and rapid generation of genetic variants |
| title_full | Highly efficient and rapid generation of genetic variants |
| title_fullStr | Highly efficient and rapid generation of genetic variants |
| title_full_unstemmed | Highly efficient and rapid generation of genetic variants |
| title_short | Highly efficient and rapid generation of genetic variants |
| title_sort | highly efficient and rapid generation of genetic variants |
| topic | Research Briefing |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10158673/ https://www.ncbi.nlm.nih.gov/pubmed/37142768 http://dx.doi.org/10.1038/s41592-023-01869-0 |