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Enhanced PD-L1 expression on tumor cells in primary CD30-positive cutaneous large T-cell lymphoma: a report of lymph node lesions of four cases
Scarce data are available regarding neoplastic PD-L1 (nPD-L1, clone SP142) expression in cutaneous T-cell lymphoma. We recently documented a possible association of increased nPD-L1 expression with tumor progression to secondary nodal involvement in two cases of CD30-positive primary cutaneous large...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
JSLRT
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10158725/ https://www.ncbi.nlm.nih.gov/pubmed/36990776 http://dx.doi.org/10.3960/jslrt.22042 |
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author | Takahashi, Emiko Imai, Hiroshi Tsuyuki, Yuta Taniguchi, Natsuki Kogure, Yasunori Kataoka, Keisuke Tsuchida, Takashi Baba, Satoshi Tsuzuki, Toyonori Shimauchi, Takatoshi Nakamura, Shigeo |
author_facet | Takahashi, Emiko Imai, Hiroshi Tsuyuki, Yuta Taniguchi, Natsuki Kogure, Yasunori Kataoka, Keisuke Tsuchida, Takashi Baba, Satoshi Tsuzuki, Toyonori Shimauchi, Takatoshi Nakamura, Shigeo |
author_sort | Takahashi, Emiko |
collection | PubMed |
description | Scarce data are available regarding neoplastic PD-L1 (nPD-L1, clone SP142) expression in cutaneous T-cell lymphoma. We recently documented a possible association of increased nPD-L1 expression with tumor progression to secondary nodal involvement in two cases of CD30-positive primary cutaneous large T-cell lymphoma (PC-LTCL) (Pathol Int 2020;70:804). Notably, the nodal sites exhibited classic Hodgkin lymphoma (CHL) mimicry related to both morphology and tumor microenvironment (TME), i.e., abundant PD-L1-positive tumor-associated macrophages and low-level PD-1 expression on T-cells. Immunohistochemistry highlighted distinctly different nPD-L1 positivity between the cutaneous and nodal lesions. In the present study, we aimed to validate this unique phenomenon in a larger series of four cases with FISH and targeted-capture sequencing (targeted-seq) analysis. We retrospectively identified two more cases of CD30-positive PC-LTCL with secondary nodal involvement among all patients consecutively diagnosed between 2001–2021. All cases immunohistochemically exhibited elevated nPD-L1 expression on ≥50% of lymphoma cells in nodal tumors, clearly contrasting with the scarce nPD-L1 positivity (≤1%) in cutaneous tumors. Moreover, all nodal lesions exhibited CHL-like TME, with abundant PD-L1-positive tumor-associated macrophages and low-level PD-1 expression on T cells, although the CHL-like morphology was limited in the two original cases. None showed CD274/PD-L1 copy number alteration by FISH analysis, or structural variations of PD-L1 3’-UTR by targeted-seq analysis. These findings indicated that nPD-L1 expression is linked with tumor progression and CHL-like TME in nodal involvement of PC-LTCL. Interestingly, one autopsied case exhibited heterogeneity of nPD-L1 expression at different disease sites. |
format | Online Article Text |
id | pubmed-10158725 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | JSLRT |
record_format | MEDLINE/PubMed |
spelling | pubmed-101587252023-05-05 Enhanced PD-L1 expression on tumor cells in primary CD30-positive cutaneous large T-cell lymphoma: a report of lymph node lesions of four cases Takahashi, Emiko Imai, Hiroshi Tsuyuki, Yuta Taniguchi, Natsuki Kogure, Yasunori Kataoka, Keisuke Tsuchida, Takashi Baba, Satoshi Tsuzuki, Toyonori Shimauchi, Takatoshi Nakamura, Shigeo J Clin Exp Hematop Case Report Scarce data are available regarding neoplastic PD-L1 (nPD-L1, clone SP142) expression in cutaneous T-cell lymphoma. We recently documented a possible association of increased nPD-L1 expression with tumor progression to secondary nodal involvement in two cases of CD30-positive primary cutaneous large T-cell lymphoma (PC-LTCL) (Pathol Int 2020;70:804). Notably, the nodal sites exhibited classic Hodgkin lymphoma (CHL) mimicry related to both morphology and tumor microenvironment (TME), i.e., abundant PD-L1-positive tumor-associated macrophages and low-level PD-1 expression on T-cells. Immunohistochemistry highlighted distinctly different nPD-L1 positivity between the cutaneous and nodal lesions. In the present study, we aimed to validate this unique phenomenon in a larger series of four cases with FISH and targeted-capture sequencing (targeted-seq) analysis. We retrospectively identified two more cases of CD30-positive PC-LTCL with secondary nodal involvement among all patients consecutively diagnosed between 2001–2021. All cases immunohistochemically exhibited elevated nPD-L1 expression on ≥50% of lymphoma cells in nodal tumors, clearly contrasting with the scarce nPD-L1 positivity (≤1%) in cutaneous tumors. Moreover, all nodal lesions exhibited CHL-like TME, with abundant PD-L1-positive tumor-associated macrophages and low-level PD-1 expression on T cells, although the CHL-like morphology was limited in the two original cases. None showed CD274/PD-L1 copy number alteration by FISH analysis, or structural variations of PD-L1 3’-UTR by targeted-seq analysis. These findings indicated that nPD-L1 expression is linked with tumor progression and CHL-like TME in nodal involvement of PC-LTCL. Interestingly, one autopsied case exhibited heterogeneity of nPD-L1 expression at different disease sites. JSLRT 2023-03-28 /pmc/articles/PMC10158725/ /pubmed/36990776 http://dx.doi.org/10.3960/jslrt.22042 Text en © 2023 by The Japanese Society for Lymphoreticular Tissue Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution ShareAlike (CC BY-NC-SA) 4.0 License. |
spellingShingle | Case Report Takahashi, Emiko Imai, Hiroshi Tsuyuki, Yuta Taniguchi, Natsuki Kogure, Yasunori Kataoka, Keisuke Tsuchida, Takashi Baba, Satoshi Tsuzuki, Toyonori Shimauchi, Takatoshi Nakamura, Shigeo Enhanced PD-L1 expression on tumor cells in primary CD30-positive cutaneous large T-cell lymphoma: a report of lymph node lesions of four cases |
title | Enhanced PD-L1 expression on tumor cells in primary CD30-positive cutaneous large T-cell lymphoma: a report of lymph node lesions of four cases |
title_full | Enhanced PD-L1 expression on tumor cells in primary CD30-positive cutaneous large T-cell lymphoma: a report of lymph node lesions of four cases |
title_fullStr | Enhanced PD-L1 expression on tumor cells in primary CD30-positive cutaneous large T-cell lymphoma: a report of lymph node lesions of four cases |
title_full_unstemmed | Enhanced PD-L1 expression on tumor cells in primary CD30-positive cutaneous large T-cell lymphoma: a report of lymph node lesions of four cases |
title_short | Enhanced PD-L1 expression on tumor cells in primary CD30-positive cutaneous large T-cell lymphoma: a report of lymph node lesions of four cases |
title_sort | enhanced pd-l1 expression on tumor cells in primary cd30-positive cutaneous large t-cell lymphoma: a report of lymph node lesions of four cases |
topic | Case Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10158725/ https://www.ncbi.nlm.nih.gov/pubmed/36990776 http://dx.doi.org/10.3960/jslrt.22042 |
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