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Ocimum tenuiflorum extract (HOLIXER(TM)): Possible effects on hypothalamic–pituitary–adrenal (HPA) axis in modulating stress

Ocimum tenuiflorum is a sacred medicinal plant bestowed with multiple health benefits. This plant is traditionally considered an adaptogen. Many scientific studies have indicated the anti-stress potential of Ocimum tenuiflorum but with higher doses. The present study investigated the effects of Holi...

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Autores principales: C. M., Mohan Gowda, Murugan, Sasi Kumar, Bethapudi, Bharathi, Purusothaman, Divya, Mundkinajeddu, Deepak, D’Souza, Prashanth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10159140/
https://www.ncbi.nlm.nih.gov/pubmed/37141281
http://dx.doi.org/10.1371/journal.pone.0285012
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author C. M., Mohan Gowda
Murugan, Sasi Kumar
Bethapudi, Bharathi
Purusothaman, Divya
Mundkinajeddu, Deepak
D’Souza, Prashanth
author_facet C. M., Mohan Gowda
Murugan, Sasi Kumar
Bethapudi, Bharathi
Purusothaman, Divya
Mundkinajeddu, Deepak
D’Souza, Prashanth
author_sort C. M., Mohan Gowda
collection PubMed
description Ocimum tenuiflorum is a sacred medicinal plant bestowed with multiple health benefits. This plant is traditionally considered an adaptogen. Many scientific studies have indicated the anti-stress potential of Ocimum tenuiflorum but with higher doses. The present study investigated the effects of Holixer(TM) (a clinically studied standardized Ocimum tenuiflorum extract) on modulating stress using two in vivo models, namely the swim endurance study in mice and forced swim test in rats. In addition, we explored the mechanism of action of Holixer(TM) on the HPA axis using two in vitro cell-based assays to check for its inhibitory effect on cortisol release and CRF1 receptor antagonistic activity. Ocimum tenuiflorum extract enhanced the swimming time in mice, reduced the stress-induced increase in immobility time, and prevented the increase in corticosterone in rats subjected to the forced swim test. Further, Ocimum tenuiflorum extract inhibited cortisol release and exhibited a significant CRF1 receptor antagonist activity. Thus, Ocimum tenuiflorum extract was found effective in managing stress, and the effect could be due to the inhibition of cortisol release and the antagonistic effect on the CRF1 receptors.
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spelling pubmed-101591402023-05-05 Ocimum tenuiflorum extract (HOLIXER(TM)): Possible effects on hypothalamic–pituitary–adrenal (HPA) axis in modulating stress C. M., Mohan Gowda Murugan, Sasi Kumar Bethapudi, Bharathi Purusothaman, Divya Mundkinajeddu, Deepak D’Souza, Prashanth PLoS One Research Article Ocimum tenuiflorum is a sacred medicinal plant bestowed with multiple health benefits. This plant is traditionally considered an adaptogen. Many scientific studies have indicated the anti-stress potential of Ocimum tenuiflorum but with higher doses. The present study investigated the effects of Holixer(TM) (a clinically studied standardized Ocimum tenuiflorum extract) on modulating stress using two in vivo models, namely the swim endurance study in mice and forced swim test in rats. In addition, we explored the mechanism of action of Holixer(TM) on the HPA axis using two in vitro cell-based assays to check for its inhibitory effect on cortisol release and CRF1 receptor antagonistic activity. Ocimum tenuiflorum extract enhanced the swimming time in mice, reduced the stress-induced increase in immobility time, and prevented the increase in corticosterone in rats subjected to the forced swim test. Further, Ocimum tenuiflorum extract inhibited cortisol release and exhibited a significant CRF1 receptor antagonist activity. Thus, Ocimum tenuiflorum extract was found effective in managing stress, and the effect could be due to the inhibition of cortisol release and the antagonistic effect on the CRF1 receptors. Public Library of Science 2023-05-04 /pmc/articles/PMC10159140/ /pubmed/37141281 http://dx.doi.org/10.1371/journal.pone.0285012 Text en © 2023 C. M. et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
C. M., Mohan Gowda
Murugan, Sasi Kumar
Bethapudi, Bharathi
Purusothaman, Divya
Mundkinajeddu, Deepak
D’Souza, Prashanth
Ocimum tenuiflorum extract (HOLIXER(TM)): Possible effects on hypothalamic–pituitary–adrenal (HPA) axis in modulating stress
title Ocimum tenuiflorum extract (HOLIXER(TM)): Possible effects on hypothalamic–pituitary–adrenal (HPA) axis in modulating stress
title_full Ocimum tenuiflorum extract (HOLIXER(TM)): Possible effects on hypothalamic–pituitary–adrenal (HPA) axis in modulating stress
title_fullStr Ocimum tenuiflorum extract (HOLIXER(TM)): Possible effects on hypothalamic–pituitary–adrenal (HPA) axis in modulating stress
title_full_unstemmed Ocimum tenuiflorum extract (HOLIXER(TM)): Possible effects on hypothalamic–pituitary–adrenal (HPA) axis in modulating stress
title_short Ocimum tenuiflorum extract (HOLIXER(TM)): Possible effects on hypothalamic–pituitary–adrenal (HPA) axis in modulating stress
title_sort ocimum tenuiflorum extract (holixer(tm)): possible effects on hypothalamic–pituitary–adrenal (hpa) axis in modulating stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10159140/
https://www.ncbi.nlm.nih.gov/pubmed/37141281
http://dx.doi.org/10.1371/journal.pone.0285012
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