A hybrid technique for measurement of intra/extracellular proteins

ELISA or Western blot is known as a basic technique to be used for measurement of intracellular proteins, but in some cases, they cannot overcome problems such as normalization between samples or extraneous costs for required commercial kits. In order to address this problem, we developed a rapid an...

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Detalles Bibliográficos
Autores principales: Edabashi, Haiam, Elghadafi, Radwa, Rajkanth, Nischwethaa, Mody, Janvi Saurabh, Ma, Weiyuan, Dibart, Serge, Tang, Xiaoren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10159171/
https://www.ncbi.nlm.nih.gov/pubmed/37141290
http://dx.doi.org/10.1371/journal.pone.0282948
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author Edabashi, Haiam
Elghadafi, Radwa
Rajkanth, Nischwethaa
Mody, Janvi Saurabh
Ma, Weiyuan
Dibart, Serge
Tang, Xiaoren
author_facet Edabashi, Haiam
Elghadafi, Radwa
Rajkanth, Nischwethaa
Mody, Janvi Saurabh
Ma, Weiyuan
Dibart, Serge
Tang, Xiaoren
author_sort Edabashi, Haiam
collection PubMed
description ELISA or Western blot is known as a basic technique to be used for measurement of intracellular proteins, but in some cases, they cannot overcome problems such as normalization between samples or extraneous costs for required commercial kits. In order to address this problem, we developed a rapid and effective method (a hybrid of Western blot and ELISA). We use this new hybrid method to detect and normalize trace protein changes in gene expression intracellularly at a lower cost.
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spelling pubmed-101591712023-05-05 A hybrid technique for measurement of intra/extracellular proteins Edabashi, Haiam Elghadafi, Radwa Rajkanth, Nischwethaa Mody, Janvi Saurabh Ma, Weiyuan Dibart, Serge Tang, Xiaoren PLoS One Research Article ELISA or Western blot is known as a basic technique to be used for measurement of intracellular proteins, but in some cases, they cannot overcome problems such as normalization between samples or extraneous costs for required commercial kits. In order to address this problem, we developed a rapid and effective method (a hybrid of Western blot and ELISA). We use this new hybrid method to detect and normalize trace protein changes in gene expression intracellularly at a lower cost. Public Library of Science 2023-05-04 /pmc/articles/PMC10159171/ /pubmed/37141290 http://dx.doi.org/10.1371/journal.pone.0282948 Text en © 2023 Edabashi et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Edabashi, Haiam
Elghadafi, Radwa
Rajkanth, Nischwethaa
Mody, Janvi Saurabh
Ma, Weiyuan
Dibart, Serge
Tang, Xiaoren
A hybrid technique for measurement of intra/extracellular proteins
title A hybrid technique for measurement of intra/extracellular proteins
title_full A hybrid technique for measurement of intra/extracellular proteins
title_fullStr A hybrid technique for measurement of intra/extracellular proteins
title_full_unstemmed A hybrid technique for measurement of intra/extracellular proteins
title_short A hybrid technique for measurement of intra/extracellular proteins
title_sort hybrid technique for measurement of intra/extracellular proteins
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10159171/
https://www.ncbi.nlm.nih.gov/pubmed/37141290
http://dx.doi.org/10.1371/journal.pone.0282948
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