Cargando…

A high-quality chromosomal-level genome assembly of Greater Scaup (Aythya marila)

Aythya marila is one of the few species of Anatidae, and the only Aythya to live in the circumpolar. However, there is a relative lack of research on genetics of this species. In this study, we reported and assembled the first high-quality chromosome-level genome assembly of A. marila. This genome w...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Shengyang, Xia, Tian, Gao, Xiaodong, Lyu, Tianshu, Wang, Lidong, Wang, Xibao, Shi, Lupeng, Dong, Yuehuan, Zhang, Honghai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160052/
https://www.ncbi.nlm.nih.gov/pubmed/37142629
http://dx.doi.org/10.1038/s41597-023-02142-x
_version_ 1785037203117703168
author Zhou, Shengyang
Xia, Tian
Gao, Xiaodong
Lyu, Tianshu
Wang, Lidong
Wang, Xibao
Shi, Lupeng
Dong, Yuehuan
Zhang, Honghai
author_facet Zhou, Shengyang
Xia, Tian
Gao, Xiaodong
Lyu, Tianshu
Wang, Lidong
Wang, Xibao
Shi, Lupeng
Dong, Yuehuan
Zhang, Honghai
author_sort Zhou, Shengyang
collection PubMed
description Aythya marila is one of the few species of Anatidae, and the only Aythya to live in the circumpolar. However, there is a relative lack of research on genetics of this species. In this study, we reported and assembled the first high-quality chromosome-level genome assembly of A. marila. This genome was assembled using Nanopore long reads, and errors corrected using Illumina short reads, with a final genome size of 1.14 Gb, scaffold N50 of 85.44 Mb, and contig N50 of 32.46 Mb. 106 contigs were clustered and ordered onto 35 chromosomes based on Hi-C data, covering approximately 98.28% of the genome. BUSCO assessment showed that 97.0% of the highly conserved genes in aves_odb10 were present intact in the genome assembly. In addition, a total of 154.94 Mb of repetitive sequences were identified. 15,953 protein-coding genes were predicted in the genome, and 98.96% of genes were functionally annotated. This genome will be a valuable resource for future genetic diversity and genomics studies of A. marila.
format Online
Article
Text
id pubmed-10160052
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-101600522023-05-06 A high-quality chromosomal-level genome assembly of Greater Scaup (Aythya marila) Zhou, Shengyang Xia, Tian Gao, Xiaodong Lyu, Tianshu Wang, Lidong Wang, Xibao Shi, Lupeng Dong, Yuehuan Zhang, Honghai Sci Data Data Descriptor Aythya marila is one of the few species of Anatidae, and the only Aythya to live in the circumpolar. However, there is a relative lack of research on genetics of this species. In this study, we reported and assembled the first high-quality chromosome-level genome assembly of A. marila. This genome was assembled using Nanopore long reads, and errors corrected using Illumina short reads, with a final genome size of 1.14 Gb, scaffold N50 of 85.44 Mb, and contig N50 of 32.46 Mb. 106 contigs were clustered and ordered onto 35 chromosomes based on Hi-C data, covering approximately 98.28% of the genome. BUSCO assessment showed that 97.0% of the highly conserved genes in aves_odb10 were present intact in the genome assembly. In addition, a total of 154.94 Mb of repetitive sequences were identified. 15,953 protein-coding genes were predicted in the genome, and 98.96% of genes were functionally annotated. This genome will be a valuable resource for future genetic diversity and genomics studies of A. marila. Nature Publishing Group UK 2023-05-04 /pmc/articles/PMC10160052/ /pubmed/37142629 http://dx.doi.org/10.1038/s41597-023-02142-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Data Descriptor
Zhou, Shengyang
Xia, Tian
Gao, Xiaodong
Lyu, Tianshu
Wang, Lidong
Wang, Xibao
Shi, Lupeng
Dong, Yuehuan
Zhang, Honghai
A high-quality chromosomal-level genome assembly of Greater Scaup (Aythya marila)
title A high-quality chromosomal-level genome assembly of Greater Scaup (Aythya marila)
title_full A high-quality chromosomal-level genome assembly of Greater Scaup (Aythya marila)
title_fullStr A high-quality chromosomal-level genome assembly of Greater Scaup (Aythya marila)
title_full_unstemmed A high-quality chromosomal-level genome assembly of Greater Scaup (Aythya marila)
title_short A high-quality chromosomal-level genome assembly of Greater Scaup (Aythya marila)
title_sort high-quality chromosomal-level genome assembly of greater scaup (aythya marila)
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160052/
https://www.ncbi.nlm.nih.gov/pubmed/37142629
http://dx.doi.org/10.1038/s41597-023-02142-x
work_keys_str_mv AT zhoushengyang ahighqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT xiatian ahighqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT gaoxiaodong ahighqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT lyutianshu ahighqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT wanglidong ahighqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT wangxibao ahighqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT shilupeng ahighqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT dongyuehuan ahighqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT zhanghonghai ahighqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT zhoushengyang highqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT xiatian highqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT gaoxiaodong highqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT lyutianshu highqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT wanglidong highqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT wangxibao highqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT shilupeng highqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT dongyuehuan highqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila
AT zhanghonghai highqualitychromosomallevelgenomeassemblyofgreaterscaupaythyamarila