Cargando…
Full characterization of the three pathways of the complement system in patients with systemic lupus erythematosus
BACKGROUND: To date a complete characterization of the components of the complement (C) pathways (CLassical, LEctin and ALternative) in patients with systemic lupus erythematosus (SLE) has not been performed. We aimed to assess the function of these three C cascades through functional assays and the...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160460/ https://www.ncbi.nlm.nih.gov/pubmed/37153614 http://dx.doi.org/10.3389/fimmu.2023.1167055 |
_version_ | 1785037283458547712 |
---|---|
author | García-González, María Gómez-Bernal, Fuensanta Quevedo-Abeledo, Juan C. Fernández-Cladera, Yolanda González-Rivero, Agustín F. de Vera-González, Antonia de la Rua-Figueroa, Iñigo López-Mejias, Raquel Díaz-González, Federico González-Gay, Miguel Á. Ferraz-Amaro, Iván |
author_facet | García-González, María Gómez-Bernal, Fuensanta Quevedo-Abeledo, Juan C. Fernández-Cladera, Yolanda González-Rivero, Agustín F. de Vera-González, Antonia de la Rua-Figueroa, Iñigo López-Mejias, Raquel Díaz-González, Federico González-Gay, Miguel Á. Ferraz-Amaro, Iván |
author_sort | García-González, María |
collection | PubMed |
description | BACKGROUND: To date a complete characterization of the components of the complement (C) pathways (CLassical, LEctin and ALternative) in patients with systemic lupus erythematosus (SLE) has not been performed. We aimed to assess the function of these three C cascades through functional assays and the measurement of individual C proteins. We then studied how they relate to clinical characteristics. METHODS: New generation functional assays of the three pathways of the C system were assessed in 284 patients with SLE. Linear regression analysis was performed to study the relationship between the activity, severity, and damage of the disease and C system. RESULTS: Lower values of the functional tests AL and LE were more frequent than those of the CL pathway. Clinical activity was not related to inferior values of C routes functional assays. The presence of increased DNA binding was negatively linked to all three C pathways and products, except for C1-inh and C3a which were positively related. Disease damage revealed a consistent positive, rather than a negative, relationship with pathways and C elements. Anti-ribosomes and anti-nucleosomes were the autoantibodies that showed a greater relationship with C activation, mainly due to the LE and CL pathways. Regarding antiphospholipid antibodies, the most related with C activation were IgG anti-β2GP, predominantly involving the AL pathway. CONCLUSION: Not only the CL route, but also the AL and LE are related to SLE features. C expression patterns are linked to disease profiles. While accrual damage was associated with higher functional tests of C pathways, anti-DNA, anti-ribosomes and anti-nucleosomes antibodies, were the ones that showed a higher relationship with C activation, mainly due to the LE and CL pathways. |
format | Online Article Text |
id | pubmed-10160460 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101604602023-05-06 Full characterization of the three pathways of the complement system in patients with systemic lupus erythematosus García-González, María Gómez-Bernal, Fuensanta Quevedo-Abeledo, Juan C. Fernández-Cladera, Yolanda González-Rivero, Agustín F. de Vera-González, Antonia de la Rua-Figueroa, Iñigo López-Mejias, Raquel Díaz-González, Federico González-Gay, Miguel Á. Ferraz-Amaro, Iván Front Immunol Immunology BACKGROUND: To date a complete characterization of the components of the complement (C) pathways (CLassical, LEctin and ALternative) in patients with systemic lupus erythematosus (SLE) has not been performed. We aimed to assess the function of these three C cascades through functional assays and the measurement of individual C proteins. We then studied how they relate to clinical characteristics. METHODS: New generation functional assays of the three pathways of the C system were assessed in 284 patients with SLE. Linear regression analysis was performed to study the relationship between the activity, severity, and damage of the disease and C system. RESULTS: Lower values of the functional tests AL and LE were more frequent than those of the CL pathway. Clinical activity was not related to inferior values of C routes functional assays. The presence of increased DNA binding was negatively linked to all three C pathways and products, except for C1-inh and C3a which were positively related. Disease damage revealed a consistent positive, rather than a negative, relationship with pathways and C elements. Anti-ribosomes and anti-nucleosomes were the autoantibodies that showed a greater relationship with C activation, mainly due to the LE and CL pathways. Regarding antiphospholipid antibodies, the most related with C activation were IgG anti-β2GP, predominantly involving the AL pathway. CONCLUSION: Not only the CL route, but also the AL and LE are related to SLE features. C expression patterns are linked to disease profiles. While accrual damage was associated with higher functional tests of C pathways, anti-DNA, anti-ribosomes and anti-nucleosomes antibodies, were the ones that showed a higher relationship with C activation, mainly due to the LE and CL pathways. Frontiers Media S.A. 2023-04-21 /pmc/articles/PMC10160460/ /pubmed/37153614 http://dx.doi.org/10.3389/fimmu.2023.1167055 Text en Copyright © 2023 García-González, Gómez-Bernal, Quevedo-Abeledo, Fernández-Cladera, González-Rivero, de Vera-González, de la Rua-Figueroa, López-Mejias, Díaz-González, González-Gay and Ferraz-Amaro https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology García-González, María Gómez-Bernal, Fuensanta Quevedo-Abeledo, Juan C. Fernández-Cladera, Yolanda González-Rivero, Agustín F. de Vera-González, Antonia de la Rua-Figueroa, Iñigo López-Mejias, Raquel Díaz-González, Federico González-Gay, Miguel Á. Ferraz-Amaro, Iván Full characterization of the three pathways of the complement system in patients with systemic lupus erythematosus |
title | Full characterization of the three pathways of the complement system in patients with systemic lupus erythematosus |
title_full | Full characterization of the three pathways of the complement system in patients with systemic lupus erythematosus |
title_fullStr | Full characterization of the three pathways of the complement system in patients with systemic lupus erythematosus |
title_full_unstemmed | Full characterization of the three pathways of the complement system in patients with systemic lupus erythematosus |
title_short | Full characterization of the three pathways of the complement system in patients with systemic lupus erythematosus |
title_sort | full characterization of the three pathways of the complement system in patients with systemic lupus erythematosus |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160460/ https://www.ncbi.nlm.nih.gov/pubmed/37153614 http://dx.doi.org/10.3389/fimmu.2023.1167055 |
work_keys_str_mv | AT garciagonzalezmaria fullcharacterizationofthethreepathwaysofthecomplementsysteminpatientswithsystemiclupuserythematosus AT gomezbernalfuensanta fullcharacterizationofthethreepathwaysofthecomplementsysteminpatientswithsystemiclupuserythematosus AT quevedoabeledojuanc fullcharacterizationofthethreepathwaysofthecomplementsysteminpatientswithsystemiclupuserythematosus AT fernandezcladerayolanda fullcharacterizationofthethreepathwaysofthecomplementsysteminpatientswithsystemiclupuserythematosus AT gonzalezriveroagustinf fullcharacterizationofthethreepathwaysofthecomplementsysteminpatientswithsystemiclupuserythematosus AT deveragonzalezantonia fullcharacterizationofthethreepathwaysofthecomplementsysteminpatientswithsystemiclupuserythematosus AT delaruafigueroainigo fullcharacterizationofthethreepathwaysofthecomplementsysteminpatientswithsystemiclupuserythematosus AT lopezmejiasraquel fullcharacterizationofthethreepathwaysofthecomplementsysteminpatientswithsystemiclupuserythematosus AT diazgonzalezfederico fullcharacterizationofthethreepathwaysofthecomplementsysteminpatientswithsystemiclupuserythematosus AT gonzalezgaymiguela fullcharacterizationofthethreepathwaysofthecomplementsysteminpatientswithsystemiclupuserythematosus AT ferrazamaroivan fullcharacterizationofthethreepathwaysofthecomplementsysteminpatientswithsystemiclupuserythematosus |