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Modification of the serotonergic systems and phenotypes by gestational micronutrients

Micronutrients consumed in excess or imbalanced amounts during pregnancy may increase the risk of metabolic diseases in offspring, but the mechanisms underlying these effects are unknown. Serotonin (5-hydroxytryptamine, 5-HT), a multifunctional indoleamine in the brain and the gut, may have key role...

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Autores principales: Chen, Vicki, Shelp, Gia V, Schwartz, Jacob L, Aardema, Niklas D J, Bunnell, Madison L, Cho, Clara E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bioscientifica Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160553/
https://www.ncbi.nlm.nih.gov/pubmed/36930294
http://dx.doi.org/10.1530/JOE-22-0305
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author Chen, Vicki
Shelp, Gia V
Schwartz, Jacob L
Aardema, Niklas D J
Bunnell, Madison L
Cho, Clara E
author_facet Chen, Vicki
Shelp, Gia V
Schwartz, Jacob L
Aardema, Niklas D J
Bunnell, Madison L
Cho, Clara E
author_sort Chen, Vicki
collection PubMed
description Micronutrients consumed in excess or imbalanced amounts during pregnancy may increase the risk of metabolic diseases in offspring, but the mechanisms underlying these effects are unknown. Serotonin (5-hydroxytryptamine, 5-HT), a multifunctional indoleamine in the brain and the gut, may have key roles in regulating metabolism. We investigated the effects of gestational micronutrient intakes on the central and peripheral serotonergic systems as modulators of the offspring's metabolic phenotypes. Pregnant Wistar rats were fed an AIN-93G diet with 1-fold recommended vitamins (RV), high 10-fold multivitamins (HV), high 10-fold folic acid with recommended choline (HFolRC), or high 10-fold folic acid with no choline (HFolNC). Male and female offspring were weaned to a high-fat RV diet for 12 weeks. We assessed the central function using the 5-HT(2C) receptor agonist, 1-(3-chlorophenyl)piperazine (mCPP), and found that male offspring from the HV- or HFolRC-fed dams were less responsive (P < 0.05) whereas female HFolRC offspring were more responsive to mCPP (P < 0.01) at 6 weeks post-weaning. Male and female offspring from the HV and HFolNC groups, and male HFolRC offspring had greater food intake (males P < 0.001; females P < 0.001) and weight gain (males P < 0.0001; females P < 0.0001), elevated colon 5-HT (males P < 0.01; females P < 0.001) and fasting glucose concentrations (males P < 0.01; females P < 0.01), as well as body composition toward obesity (males P < 0.01; females P < 0.01) at 12 weeks post-weaning. Colon 5-HT was correlated with fasting glucose concentrations (males R(2)=0.78, P < 0.0001; females R(2)=0.71, P < 0.0001). Overall, the serotonergic systems are sensitive to the composition of gestational micronutrients, with alterations consistent with metabolic disturbances in offspring.
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spelling pubmed-101605532023-05-06 Modification of the serotonergic systems and phenotypes by gestational micronutrients Chen, Vicki Shelp, Gia V Schwartz, Jacob L Aardema, Niklas D J Bunnell, Madison L Cho, Clara E J Endocrinol Research Micronutrients consumed in excess or imbalanced amounts during pregnancy may increase the risk of metabolic diseases in offspring, but the mechanisms underlying these effects are unknown. Serotonin (5-hydroxytryptamine, 5-HT), a multifunctional indoleamine in the brain and the gut, may have key roles in regulating metabolism. We investigated the effects of gestational micronutrient intakes on the central and peripheral serotonergic systems as modulators of the offspring's metabolic phenotypes. Pregnant Wistar rats were fed an AIN-93G diet with 1-fold recommended vitamins (RV), high 10-fold multivitamins (HV), high 10-fold folic acid with recommended choline (HFolRC), or high 10-fold folic acid with no choline (HFolNC). Male and female offspring were weaned to a high-fat RV diet for 12 weeks. We assessed the central function using the 5-HT(2C) receptor agonist, 1-(3-chlorophenyl)piperazine (mCPP), and found that male offspring from the HV- or HFolRC-fed dams were less responsive (P < 0.05) whereas female HFolRC offspring were more responsive to mCPP (P < 0.01) at 6 weeks post-weaning. Male and female offspring from the HV and HFolNC groups, and male HFolRC offspring had greater food intake (males P < 0.001; females P < 0.001) and weight gain (males P < 0.0001; females P < 0.0001), elevated colon 5-HT (males P < 0.01; females P < 0.001) and fasting glucose concentrations (males P < 0.01; females P < 0.01), as well as body composition toward obesity (males P < 0.01; females P < 0.01) at 12 weeks post-weaning. Colon 5-HT was correlated with fasting glucose concentrations (males R(2)=0.78, P < 0.0001; females R(2)=0.71, P < 0.0001). Overall, the serotonergic systems are sensitive to the composition of gestational micronutrients, with alterations consistent with metabolic disturbances in offspring. Bioscientifica Ltd 2023-03-16 /pmc/articles/PMC10160553/ /pubmed/36930294 http://dx.doi.org/10.1530/JOE-22-0305 Text en © the author(s) https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License. (https://creativecommons.org/licenses/by/4.0/)
spellingShingle Research
Chen, Vicki
Shelp, Gia V
Schwartz, Jacob L
Aardema, Niklas D J
Bunnell, Madison L
Cho, Clara E
Modification of the serotonergic systems and phenotypes by gestational micronutrients
title Modification of the serotonergic systems and phenotypes by gestational micronutrients
title_full Modification of the serotonergic systems and phenotypes by gestational micronutrients
title_fullStr Modification of the serotonergic systems and phenotypes by gestational micronutrients
title_full_unstemmed Modification of the serotonergic systems and phenotypes by gestational micronutrients
title_short Modification of the serotonergic systems and phenotypes by gestational micronutrients
title_sort modification of the serotonergic systems and phenotypes by gestational micronutrients
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160553/
https://www.ncbi.nlm.nih.gov/pubmed/36930294
http://dx.doi.org/10.1530/JOE-22-0305
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