Protocol for analyzing transforming growth factor β signaling in dextran-sulfate-sodium-induced colitic mice using flow cytometry and western blotting

Transforming growth factor β (TGF-β) is critical to the maintenance of intestinal immune homeostasis. Here, we present techniques for analyzing Smad molecules downstream of TGF-β receptor signaling in dextran-sulfate-sodium-induced colitic mice. We describe colitis induction, cell isolation, and flo...

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Detalles Bibliográficos
Autores principales: Fujiwara, Mai, Garo, Lucien, Ajay, Amrendra K., Cannon, Alkeiver S., Kolypetri, Panagiota, Dhuppar, Shivnarayan, Murugaiyan, Gopal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160580/
https://www.ncbi.nlm.nih.gov/pubmed/37099428
http://dx.doi.org/10.1016/j.xpro.2023.102249

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