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Protocol for labeling epileptic-status-related neuronal ensembles in mouse hippocampal kindling model
Epileptic networks are characterized by two states, seizures or more prolonged interictal periods. Here, we present the procedure for labeling seizure-activated and interictal-activated neuronal ensembles in mouse hippocampal kindling model using an enhanced-synaptic-activity-responsive element. We...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160588/ https://www.ncbi.nlm.nih.gov/pubmed/37099430 http://dx.doi.org/10.1016/j.xpro.2023.102255 |
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author | Lai, Nanxi Li, Zhisheng Chen, Zhong Wang, Yi |
author_facet | Lai, Nanxi Li, Zhisheng Chen, Zhong Wang, Yi |
author_sort | Lai, Nanxi |
collection | PubMed |
description | Epileptic networks are characterized by two states, seizures or more prolonged interictal periods. Here, we present the procedure for labeling seizure-activated and interictal-activated neuronal ensembles in mouse hippocampal kindling model using an enhanced-synaptic-activity-responsive element. We describe the seizure model establishment, tamoxifen induction, electrical stimulation, and calcium signal recording of labeled ensembles. This protocol has demonstrated dissociated calcium activities in the two ensembles during focal seizure dynamics and can be applied to other animal models of epilepsy. For complete details on the use and execution of this protocol, please refer to Lai et al. (2022).(1) |
format | Online Article Text |
id | pubmed-10160588 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-101605882023-05-06 Protocol for labeling epileptic-status-related neuronal ensembles in mouse hippocampal kindling model Lai, Nanxi Li, Zhisheng Chen, Zhong Wang, Yi STAR Protoc Protocol Epileptic networks are characterized by two states, seizures or more prolonged interictal periods. Here, we present the procedure for labeling seizure-activated and interictal-activated neuronal ensembles in mouse hippocampal kindling model using an enhanced-synaptic-activity-responsive element. We describe the seizure model establishment, tamoxifen induction, electrical stimulation, and calcium signal recording of labeled ensembles. This protocol has demonstrated dissociated calcium activities in the two ensembles during focal seizure dynamics and can be applied to other animal models of epilepsy. For complete details on the use and execution of this protocol, please refer to Lai et al. (2022).(1) Elsevier 2023-04-25 /pmc/articles/PMC10160588/ /pubmed/37099430 http://dx.doi.org/10.1016/j.xpro.2023.102255 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Lai, Nanxi Li, Zhisheng Chen, Zhong Wang, Yi Protocol for labeling epileptic-status-related neuronal ensembles in mouse hippocampal kindling model |
title | Protocol for labeling epileptic-status-related neuronal ensembles in mouse hippocampal kindling model |
title_full | Protocol for labeling epileptic-status-related neuronal ensembles in mouse hippocampal kindling model |
title_fullStr | Protocol for labeling epileptic-status-related neuronal ensembles in mouse hippocampal kindling model |
title_full_unstemmed | Protocol for labeling epileptic-status-related neuronal ensembles in mouse hippocampal kindling model |
title_short | Protocol for labeling epileptic-status-related neuronal ensembles in mouse hippocampal kindling model |
title_sort | protocol for labeling epileptic-status-related neuronal ensembles in mouse hippocampal kindling model |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160588/ https://www.ncbi.nlm.nih.gov/pubmed/37099430 http://dx.doi.org/10.1016/j.xpro.2023.102255 |
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