Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites

Here, we focus on Leishmania extracellular vesicles (EVs) and their DNA content, detailing a protocol for the isolation of these nanoparticles and their subsequent genomic characterization. We describe a robust and comprehensive approach for obtaining, storing, and analyzing EVs derived from culture...

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Detalles Bibliográficos
Autores principales: Ibarra-Meneses, Ana Victoria, Amin, Atia, Dong, George, Olivier, Martin, Langlais, David, Fernandez-Prada, Christopher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160803/
https://www.ncbi.nlm.nih.gov/pubmed/37087735
http://dx.doi.org/10.1016/j.xpro.2023.102248
Descripción
Sumario:Here, we focus on Leishmania extracellular vesicles (EVs) and their DNA content, detailing a protocol for the isolation of these nanoparticles and their subsequent genomic characterization. We describe a robust and comprehensive approach for obtaining, storing, and analyzing EVs derived from cultured parasites. We detail a user-friendly bioinformatics pipeline for sequence analysis and visualization of CNV analysis and ploidy changes. For complete details on the use and execution of this protocol, please refer to Douanne et al. (2022).(1)

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