Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites

Here, we focus on Leishmania extracellular vesicles (EVs) and their DNA content, detailing a protocol for the isolation of these nanoparticles and their subsequent genomic characterization. We describe a robust and comprehensive approach for obtaining, storing, and analyzing EVs derived from culture...

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Detalles Bibliográficos
Autores principales: Ibarra-Meneses, Ana Victoria, Amin, Atia, Dong, George, Olivier, Martin, Langlais, David, Fernandez-Prada, Christopher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160803/
https://www.ncbi.nlm.nih.gov/pubmed/37087735
http://dx.doi.org/10.1016/j.xpro.2023.102248
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author Ibarra-Meneses, Ana Victoria
Amin, Atia
Dong, George
Olivier, Martin
Langlais, David
Fernandez-Prada, Christopher
author_facet Ibarra-Meneses, Ana Victoria
Amin, Atia
Dong, George
Olivier, Martin
Langlais, David
Fernandez-Prada, Christopher
author_sort Ibarra-Meneses, Ana Victoria
collection PubMed
description Here, we focus on Leishmania extracellular vesicles (EVs) and their DNA content, detailing a protocol for the isolation of these nanoparticles and their subsequent genomic characterization. We describe a robust and comprehensive approach for obtaining, storing, and analyzing EVs derived from cultured parasites. We detail a user-friendly bioinformatics pipeline for sequence analysis and visualization of CNV analysis and ploidy changes. For complete details on the use and execution of this protocol, please refer to Douanne et al. (2022).(1)
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spelling pubmed-101608032023-05-06 Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites Ibarra-Meneses, Ana Victoria Amin, Atia Dong, George Olivier, Martin Langlais, David Fernandez-Prada, Christopher STAR Protoc Protocol Here, we focus on Leishmania extracellular vesicles (EVs) and their DNA content, detailing a protocol for the isolation of these nanoparticles and their subsequent genomic characterization. We describe a robust and comprehensive approach for obtaining, storing, and analyzing EVs derived from cultured parasites. We detail a user-friendly bioinformatics pipeline for sequence analysis and visualization of CNV analysis and ploidy changes. For complete details on the use and execution of this protocol, please refer to Douanne et al. (2022).(1) Elsevier 2023-04-22 /pmc/articles/PMC10160803/ /pubmed/37087735 http://dx.doi.org/10.1016/j.xpro.2023.102248 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ibarra-Meneses, Ana Victoria
Amin, Atia
Dong, George
Olivier, Martin
Langlais, David
Fernandez-Prada, Christopher
Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites
title Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites
title_full Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites
title_fullStr Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites
title_full_unstemmed Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites
title_short Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites
title_sort identification and analysis of the dna content of small extracellular vesicles isolated from leishmania parasites
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160803/
https://www.ncbi.nlm.nih.gov/pubmed/37087735
http://dx.doi.org/10.1016/j.xpro.2023.102248
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