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Simple optical nanomotion method for single-bacterium viability and antibiotic response testing
Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is challenging. Recent studies demonstrated that...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160964/ https://www.ncbi.nlm.nih.gov/pubmed/37094120 http://dx.doi.org/10.1073/pnas.2221284120 |
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author | Villalba, Maria I. Rossetti, Eugenia Bonvallat, Allan Yvanoff, Charlotte Radonicic, Vjera Willaert, Ronnie G. Kasas, Sandor |
author_facet | Villalba, Maria I. Rossetti, Eugenia Bonvallat, Allan Yvanoff, Charlotte Radonicic, Vjera Willaert, Ronnie G. Kasas, Sandor |
author_sort | Villalba, Maria I. |
collection | PubMed |
description | Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is challenging. Recent studies demonstrated that monitoring bacterial nanomotion by using atomic force microscopy (AFM) upon exposure to antibiotics constitutes a rapid and highly efficient AST. Here, we present a nanomotion detection method based on optical microscopy for testing bacterial viability. This novel technique only requires a very basic microfluidic analysis chamber, and an optical microscope equipped with a camera or a mobile phone. No attachment of the microorganisms is needed, nor are specific bacterial stains or markers. This single-cell technique was successfully tested to obtain AST for motile, nonmotile, gram-positive, and gram-negative bacteria. The simplicity and efficiency of the method make it a game-changer in the field of rapid AST. |
format | Online Article Text |
id | pubmed-10160964 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-101609642023-05-06 Simple optical nanomotion method for single-bacterium viability and antibiotic response testing Villalba, Maria I. Rossetti, Eugenia Bonvallat, Allan Yvanoff, Charlotte Radonicic, Vjera Willaert, Ronnie G. Kasas, Sandor Proc Natl Acad Sci U S A Biological Sciences Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is challenging. Recent studies demonstrated that monitoring bacterial nanomotion by using atomic force microscopy (AFM) upon exposure to antibiotics constitutes a rapid and highly efficient AST. Here, we present a nanomotion detection method based on optical microscopy for testing bacterial viability. This novel technique only requires a very basic microfluidic analysis chamber, and an optical microscope equipped with a camera or a mobile phone. No attachment of the microorganisms is needed, nor are specific bacterial stains or markers. This single-cell technique was successfully tested to obtain AST for motile, nonmotile, gram-positive, and gram-negative bacteria. The simplicity and efficiency of the method make it a game-changer in the field of rapid AST. National Academy of Sciences 2023-04-24 2023-05-02 /pmc/articles/PMC10160964/ /pubmed/37094120 http://dx.doi.org/10.1073/pnas.2221284120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Villalba, Maria I. Rossetti, Eugenia Bonvallat, Allan Yvanoff, Charlotte Radonicic, Vjera Willaert, Ronnie G. Kasas, Sandor Simple optical nanomotion method for single-bacterium viability and antibiotic response testing |
title | Simple optical nanomotion method for single-bacterium viability and antibiotic response testing |
title_full | Simple optical nanomotion method for single-bacterium viability and antibiotic response testing |
title_fullStr | Simple optical nanomotion method for single-bacterium viability and antibiotic response testing |
title_full_unstemmed | Simple optical nanomotion method for single-bacterium viability and antibiotic response testing |
title_short | Simple optical nanomotion method for single-bacterium viability and antibiotic response testing |
title_sort | simple optical nanomotion method for single-bacterium viability and antibiotic response testing |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160964/ https://www.ncbi.nlm.nih.gov/pubmed/37094120 http://dx.doi.org/10.1073/pnas.2221284120 |
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