Cargando…

Simple optical nanomotion method for single-bacterium viability and antibiotic response testing

Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is challenging. Recent studies demonstrated that...

Descripción completa

Detalles Bibliográficos
Autores principales: Villalba, Maria I., Rossetti, Eugenia, Bonvallat, Allan, Yvanoff, Charlotte, Radonicic, Vjera, Willaert, Ronnie G., Kasas, Sandor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160964/
https://www.ncbi.nlm.nih.gov/pubmed/37094120
http://dx.doi.org/10.1073/pnas.2221284120
_version_ 1785037389951926272
author Villalba, Maria I.
Rossetti, Eugenia
Bonvallat, Allan
Yvanoff, Charlotte
Radonicic, Vjera
Willaert, Ronnie G.
Kasas, Sandor
author_facet Villalba, Maria I.
Rossetti, Eugenia
Bonvallat, Allan
Yvanoff, Charlotte
Radonicic, Vjera
Willaert, Ronnie G.
Kasas, Sandor
author_sort Villalba, Maria I.
collection PubMed
description Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is challenging. Recent studies demonstrated that monitoring bacterial nanomotion by using atomic force microscopy (AFM) upon exposure to antibiotics constitutes a rapid and highly efficient AST. Here, we present a nanomotion detection method based on optical microscopy for testing bacterial viability. This novel technique only requires a very basic microfluidic analysis chamber, and an optical microscope equipped with a camera or a mobile phone. No attachment of the microorganisms is needed, nor are specific bacterial stains or markers. This single-cell technique was successfully tested to obtain AST for motile, nonmotile, gram-positive, and gram-negative bacteria. The simplicity and efficiency of the method make it a game-changer in the field of rapid AST.
format Online
Article
Text
id pubmed-10160964
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher National Academy of Sciences
record_format MEDLINE/PubMed
spelling pubmed-101609642023-05-06 Simple optical nanomotion method for single-bacterium viability and antibiotic response testing Villalba, Maria I. Rossetti, Eugenia Bonvallat, Allan Yvanoff, Charlotte Radonicic, Vjera Willaert, Ronnie G. Kasas, Sandor Proc Natl Acad Sci U S A Biological Sciences Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is challenging. Recent studies demonstrated that monitoring bacterial nanomotion by using atomic force microscopy (AFM) upon exposure to antibiotics constitutes a rapid and highly efficient AST. Here, we present a nanomotion detection method based on optical microscopy for testing bacterial viability. This novel technique only requires a very basic microfluidic analysis chamber, and an optical microscope equipped with a camera or a mobile phone. No attachment of the microorganisms is needed, nor are specific bacterial stains or markers. This single-cell technique was successfully tested to obtain AST for motile, nonmotile, gram-positive, and gram-negative bacteria. The simplicity and efficiency of the method make it a game-changer in the field of rapid AST. National Academy of Sciences 2023-04-24 2023-05-02 /pmc/articles/PMC10160964/ /pubmed/37094120 http://dx.doi.org/10.1073/pnas.2221284120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Villalba, Maria I.
Rossetti, Eugenia
Bonvallat, Allan
Yvanoff, Charlotte
Radonicic, Vjera
Willaert, Ronnie G.
Kasas, Sandor
Simple optical nanomotion method for single-bacterium viability and antibiotic response testing
title Simple optical nanomotion method for single-bacterium viability and antibiotic response testing
title_full Simple optical nanomotion method for single-bacterium viability and antibiotic response testing
title_fullStr Simple optical nanomotion method for single-bacterium viability and antibiotic response testing
title_full_unstemmed Simple optical nanomotion method for single-bacterium viability and antibiotic response testing
title_short Simple optical nanomotion method for single-bacterium viability and antibiotic response testing
title_sort simple optical nanomotion method for single-bacterium viability and antibiotic response testing
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160964/
https://www.ncbi.nlm.nih.gov/pubmed/37094120
http://dx.doi.org/10.1073/pnas.2221284120
work_keys_str_mv AT villalbamariai simpleopticalnanomotionmethodforsinglebacteriumviabilityandantibioticresponsetesting
AT rossettieugenia simpleopticalnanomotionmethodforsinglebacteriumviabilityandantibioticresponsetesting
AT bonvallatallan simpleopticalnanomotionmethodforsinglebacteriumviabilityandantibioticresponsetesting
AT yvanoffcharlotte simpleopticalnanomotionmethodforsinglebacteriumviabilityandantibioticresponsetesting
AT radonicicvjera simpleopticalnanomotionmethodforsinglebacteriumviabilityandantibioticresponsetesting
AT willaertronnieg simpleopticalnanomotionmethodforsinglebacteriumviabilityandantibioticresponsetesting
AT kasassandor simpleopticalnanomotionmethodforsinglebacteriumviabilityandantibioticresponsetesting