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Membrane protein isolation and structure determination in cell-derived membrane vesicles
Integral membrane protein structure determination traditionally requires extraction from cell membranes using detergents or polymers. Here, we describe the isolation and structure determination of proteins in membrane vesicles derived directly from cells. Structures of the ion channel Slo1 from tota...
Autores principales: | Tao, Xiao, Zhao, Chen, MacKinnon, Roderick |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160969/ https://www.ncbi.nlm.nih.gov/pubmed/37098056 http://dx.doi.org/10.1073/pnas.2302325120 |
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